Mold inhibitor for fermentation bed and preparation method for mold inhibitor

A fermentation bed and inhibitor technology, applied in botany equipment and methods, fungicides, biocides, etc., can solve the problems of poor treatment effect, weak impact resistance, single component, etc., and achieve the problem of changing the balance of flora , harm reduction effect

Inactive Publication Date: 2014-11-05
燕淑海
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the mold inhibitor products for fermentation beds are mostly organic or inorganic in the market, with single ingredients, weak impact resistance and poor treatment effect

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A fermentation bed mold inhibitor, which is composed of a compound chemical agent and a compound microbial agent;

[0031] The ratio of the mass g of the compound chemical preparation and the compound microbial bacterial agent to the volume ml is 3:1;

[0032] The compound chemical preparation is composed of diatomaceous earth, sodium diacetate and potassium iodide; the mass ratio of diatomite, sodium diacetate and potassium iodide is 6:2:1;

[0033] The compound microbial bacterial agent is composed of Saccharomyces cerevisiae ( Sac-charomyces cerevisiae ) bacteria solution, Bacillus subtilis ( Bacillus subtilis ) bacteria solution, Lactobacillus delbrueckii ( L. Delbrueckii ) bacterial liquid composition; the volume ratio of the beer yeast liquid, Bacillus subtilis bacterial liquid, and Lactobacillus delbrueckii bacterial liquid is 1-2:2-4:3-6.

[0034] The compound microbial bacterial agent is obtained by pure fermentation of a single strain and compounding aft...

Embodiment 2

[0036] A preparation method for the fermentation bed mold inhibitor described in above-mentioned embodiment 1, comprises the following steps:

[0037] (1) Mix diatomaceous earth, sodium diacetate and potassium iodide in a mass ratio of 6:2:1 to obtain a compound chemical preparation;

[0038] (2) Prepare brewer's yeast liquid, Bacillus subtilis liquid and Lactobacillus delbrueckii liquid respectively;

[0039] The preparation method of the brewer's yeast liquid is as follows: the required culture medium is 10.0g of yeast extract, 20.0g of peptone, 20.0g of glucose, and 1000ml of distilled water; the culture temperature is 28-38°C; the aerobic fermentation is 24-48h to obtain beer Yeast liquid;

[0040] The preparation method of the Bacillus subtilis bacterial liquid is as follows: the required culture medium is 15.0g of peptone, 0.50g of beef extract, 20.0g of glucose, 5.0g of sodium chloride, and 1000ml of distilled water; the culture temperature is 30-36°C; aerobic fermenta...

Embodiment 3

[0045] Mold clearance experiment of mold inhibitor in fermentation bed

[0046] 1. Instruments and equipment

[0047] Air bath shaker, steam sterilizer, electric heating constant temperature incubator, watch glass, absorbent cotton ball, Erlenmeyer flask, inoculation loop, electronic balance.

[0048] 2. Experimental materials

[0049] Experimental bacteria: Saccharomyces cerevisiae ( Sac-charomyces cerevisiae ) (Provided by Institute of Microbiology, Shandong University), Bacillus subtilis ( Bacillus subtilis ) (Provided by Institute of Microbiology, Shandong University), Lactobacillus delbrueckii ( L. Delbrueckii ) (provided by Institute of Microbiology, Chinese Academy of Sciences);

[0050] Experimental chemical preparations: diatomaceous earth, sodium diacetate, potassium iodide;

[0051] Culture medium: high-salt Chapei medium in the determination of the total number of molds in feed (GB\T13092-2006);

[0052] Experimental mold material: mildew...

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Abstract

The invention discloses a mold inhibitor for a fermentation bed and a preparation method for the mold inhibitor. The mold inhibitor consists of a composite chemical preparation and a composite microbial inoculum, wherein the composite chemical preparation is prepared by mixing an organic matter and an inorganic matter, and the composite microbial inoculum is prepared by performing single-strain pure fermentation on microbes and mixing. The mold inhibitor has the advantages that by the mold inhibitor, the removal rate of molds in the fermentation bed is over 90 percent, the adsorption rate of mycotoxins is 70 percent, the harm of the molds and the mycotoxins to poultry are reduced, the problem that livestock and poultry are poisoned by the molds or the mycotoxins due to environmental factors or feed factors in the feeding process is solved, and an effect of medicinal treatment is achieved, so that the problem that intestinal florae of the livestock and the poultry are unbalanced and the problem that antibiotics are abused in the conventional breeding process of the livestock and the poultry are solved.

Description

technical field [0001] The invention relates to a composite mold inhibitor, in particular to a mold inhibitor for poultry manure fermentation beds and a preparation method thereof, belonging to the field of mold inhibitors. Background technique [0002] The development of ecological breeding has promoted the development of poultry fermentation beds, but the poultry manure contains a lot of water and the fermentation bed is very low, so mold pollution is easy to occur. Mold and mycotoxins are extremely harmful to livestock and poultry, so the research and development of mold inhibitors and mycotoxin removal agents for fermentation beds is imminent. [0003] Domestic fermented bed poultry breeding technology has just started in recent years, and no specific reports have been seen on the study of antifungal agents in fermented beds. However, a lot of work has been done on the anti-mildew work of feed at home and abroad. In order to prevent the loss of nutrients during feed sto...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/02A01N59/12A01N59/00A01N37/02A01P3/00
Inventor 燕淑海
Owner 燕淑海
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