Anti-cancer application of composition containing garlic oil, garlic total polysaccharide and garlic total saponin

A technology for total garlic saponins and garlic oil, which is applied in the field of medicine, can solve the problems of combining and not preparing the fat-soluble components of garlic, and achieves the effects of simple operation, controllable product quality and significant anti-tumor activity.

Inactive Publication Date: 2012-09-05
INST OF BASIC THEORY OF TCM CHINA ACADEMY OF CHINESE MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the current garlic medicines and health products, there is no precedent for the joint preparation of garlic fat-soluble components and water-soluble components, and there is no product that combines the above-mentioned components to fully exert the medicinal or health value of garlic.

Method used

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  • Anti-cancer application of composition containing garlic oil, garlic total polysaccharide and garlic total saponin
  • Anti-cancer application of composition containing garlic oil, garlic total polysaccharide and garlic total saponin
  • Anti-cancer application of composition containing garlic oil, garlic total polysaccharide and garlic total saponin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Example 1: Preparation of a composition comprising garlic oil, total garlic polysaccharides and total garlic saponins

[0057] 15kg of garlic with skin is crushed into particles with a diameter of about 3-5mm, put in a multifunctional extraction tank, add 7 times the amount of water to extract for 2 hours, and obtain volatile oil, medicinal liquid and medicinal residue. Use Na to collect the volatile oil in the volatile oiler 2 SO 4 Dehydration gave 30.9 mL of garlic volatile oil (oil yield 0.206%, the sum of the contents of diallyl disulfide and diallyl trisulfide in garlic oil was 52.60%). The liquid medicine is reserved.

[0058] Add 5 times the amount of water to the garlic residue, extract for 1 hour, combine the obtained medicinal solution with the medicinal solution obtained from the above-mentioned oil extraction, recover water under reduced pressure (temperature < 80°C), concentrate 1:1 (relative density at 70°C is 1.05), Add ethanol to the concentrated so...

Embodiment 2

[0067] Embodiment 2: the quality control of garlic oil (with reference to patent ZL200510083951.0)

[0068] The present embodiment adopts liquid chromatography to measure the content of diallyl trisulfide and diallyl disulfide in garlic oil, and assay method is:

[0069] 1. Instruments and materials

[0070] High-performance liquid chromatography: HP1100 liquid chromatography (including quaternary pump, diode array detector, automatic sampler, HP chemical workstation).

[0071] Chemical reagents: Methanol and formic acid are chromatographically pure, water is double distilled water, and other reagents are analytically pure. Reference substances diallyl disulfide and diallyl trisulfide are self-made, and the purity is above 98%.

[0072] 2. Chromatographic conditions

[0073] The chromatographic column is Zorbax extend C18 column (4.6×250mm 5μm); the mobile phase is methanol-0.17% (volume ratio) formic acid solution (80:20); flow rate: 1mL min-1; detection wavelength: 240n...

Embodiment 3

[0082] Embodiment 3: the quality control of total garlic saponins

[0083] In this embodiment, a UV-visible spectrophotometer is used to measure the content of total garlic saponins. Because there is no reference substance for garlic saponins at home and abroad, the inventors isolated a saponin monomer proto-iso-eruboside- B, taking this as the reference substance, adopting ultraviolet-visible spectrophotometry, using sulfuric acid-methanol as the chromogen, establishing a method for determining the content of total garlic saponins, so that the product quality is controlled. Through methodological verification, the method is accurate and reliable, and the stability (RSD%=1.1%), precision (RSD%=1.0%), repeatability (RSD%=1.52%) are all good, and the sample recovery rate is 102.59% ( RSD / =1.4%, n=6.Detect by this method, in total garlic saponin of the present invention, saponin content is not less than 40% in terms of proto-iso-eruboside-B, and concrete assay method is as foll...

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Abstract

The invention provides a composition consists based on garlic oil, garlic total polysaccharide and garlic total saponin as raw materials. The composition is integrated with water-soluble and fat-soluble effective components of garlic, so that the anti-cancer activity of the garlic is sufficiently played. The composition has excellent anti-tumor activity and has the effects of strengthening body resistance to eliminate pathogenic factors and reducing toxicity, has wide application prospect, and can be prepared into a tumor prevention medicament or health-care product with remarkable curative effect and small toxic or side effect. The invention also provides a method for preparing the composition. The method comprises the following steps: preparing the garlic oil from the garlic through double extraction; preparing garlic total polysaccharide from decoction dreg through water extraction and alcohol precipitation; and carrying out alcohol extraction and macroporous resin purification on the decoction dreg to obtain the garlic total saponin. The preparation method is simple to operate, low in cost and controllable in quality, realizes sufficient utilization of each part of garlic raw materials, and can be industrialized.

Description

technical field [0001] The invention belongs to the field of medicine, and relates to a composition comprising garlic fat-soluble components and garlic water-soluble components, in particular to a composition comprising garlic oil, garlic total polysaccharides and garlic total saponins used in the preparation of antitumor drugs and health care The application in the product, and the preparation method of this composition. Background technique [0002] Tumor is one of the major diseases that endanger human health in today's society, and has become the second leading cause of death in the world after cardiovascular and cerebrovascular diseases. The treatment plan for malignant tumors is generally combined with radiotherapy and chemotherapy after surgery. However, chemotherapy drugs have certain toxic and side effects. Traditional Chinese medicine antineoplastic drugs have unique advantages. Drugs have always been a hot research topic. [0003] Garlic has a long history of an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/8962A61P35/00A23L1/29A61K31/715A23L33/00
Inventor 刘丽梅鞠大宏柏冬刘梅洁王瑞海李玉梅
Owner INST OF BASIC THEORY OF TCM CHINA ACADEMY OF CHINESE MEDICAL SCI
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