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Medium of producing pleuromutilin by fermenting Pleurotus sp and its fermentation method

A technology of pleuromutilin and fermentation medium, which is applied in the medium and fermentation field of producing pleuromutilin by Pleuromutilus, can solve the problems of high production cost and low fermentation unit, and achieve reduced fermentation cost, high nutritional value, The effect of high yield of fungus residue

Active Publication Date: 2012-09-12
宁夏泰瑞制药股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The purpose of the present invention is to overcome the above-mentioned technical defects such as low fermentation unit of pleuromutilin and high production cost, and provide a substitute for hot-pressed soybean cake powder and soybean oil, so as to increase the fermentation unit and reduce the source of raw and auxiliary materials from being affected by the environment to the greatest extent. Factors, to ensure its sufficient supply, to achieve stable and efficient production of pleuromutilin Pleuromutilus fermentation medium for producing pleuromutilin

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Primary seed medium preparation process: within 1m 3 Add 5kg of glucose and 20kg of cold-pressed soybean cake powder to the primary seed tank 、 Dipotassium hydrogen phosphate 1kg, magnesium sulfate 100g, calcium nitrate 100g, sodium chloride 100g, silicone defoamer 300g. Sterilization is carried out after the batching is completed. Sterilization conditions: pressure 0.10-0.14MPa; temperature 120-122°C; sterilization time 31min, cooling and maintaining pressure with sterile air, the pressure is controlled at 0.01MPa. Sampling and testing showed that the pH was 5.4, the content of amino nitrogen was 18.2%, and the content of reducing sugar was 1.3%, which met the sterilization requirements of the medium and was waiting for inoculation.

[0042] Secondary seed medium preparation process: at 10m 3 Add 180kg of glucose, 100kg of cold-pressed soybean cake powder, 10kg of dipotassium hydrogen phosphate, 2kg of magnesium sulfate, 2kg of calcium nitrate, 1kg of sodium chloride...

Embodiment 2

[0050] Primary seed medium preparation process: within 1m 3 Add 12kg of glucose and 35kg of cold-pressed soybean cake powder to the primary seed tank 、 Dipotassium hydrogen phosphate 5kgL, magnesium sulfate 250g, calcium nitrate 250g, sodium chloride 150g, silicone defoamer 600g. Sterilization is carried out after the batching is completed. Sterilization conditions: pressure 0.10-0.14MPa; temperature 120-122°C; sterilization time 30min, cooling and maintaining pressure with sterile air, the pressure is controlled at 0.015MPa. Sampling and testing showed that the pH was 6.3, the content of amino nitrogen was 28.4%, and the content of reducing sugar was 3.4%, which met the sterilization requirements of the medium and was waiting for inoculation.

[0051] Secondary seed medium preparation process: at 10m 3 Add 200kg of glucose, 130kg of cold-pressed soybean cake powder, 20kg of dipotassium hydrogen phosphate, 3kg of magnesium sulfate, 3kg of calcium nitrate, 1.5kg of sodium chl...

Embodiment 3

[0059] Primary seed medium preparation process: within 1m 3 Add 20kg of glucose and 50g of cold-pressed soybean cake powder to the primary seed tank 、 Dipotassium hydrogen phosphate 10kgL, magnesium sulfate 400g, calcium nitrate 400g, sodium chloride 200g, silicone defoamer 900g. Sterilization is carried out after the batching is completed. Sterilization conditions: pressure 0.10-0.14MPa; temperature 120-122°C; sterilization time 30min, cooling and maintaining pressure with sterile air, the pressure is controlled at 0.02MPa. Sampling and testing showed that the pH was 6.5, the content of amino nitrogen was 41.4%, and the content of reducing sugar was 4.6%, which met the sterilization requirements of the medium and was waiting for inoculation.

[0060] Secondary seed medium preparation process: at 10m 3Add 220kg of glucose, 150kg of cold-pressed soybean cake powder, 40kg of dipotassium hydrogen phosphate, 4kg of magnesium sulfate, 4kg of calcium nitrate, 2kg of sodium chlorid...

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Abstract

The invention relates to a medium of producing pleuromutilin by fermenting Pleurotus sp and its fermentation method. The medium comprises a primary seed medium, a secondary seed medium and a fermentation medium, and is characterized in that the primary seed medium and the secondary seed medium contains cold pressing yellow bean cake powder and an organosilicon antifoaming agent; the fermentation seed medium contains cold pressing yellow bean cake powder, the organosilicon antifoaming agent and corn oil. According to the invention, corn oil and cold pressing bean cake powder can replace soya-bean oil and heat pressing bean cake powder, a medium formula can be optimized, the technical defects of low fermentation unit, less quantity of single pot mycelium and the like. The invention provides a fermentation unit, the thalline fermentation quality can be improved, the original accessory source can be maximally reduced without environmental influence, the sufficient supply can be guaranteed, and the production with stabilization and high efficiency of pleuromutilin can be realized.

Description

technical field [0001] The invention belongs to the technical field of fermentation, and in particular relates to a culture medium and a fermentation method for producing pleuromutilin by Pleuromutilus. Background technique [0002] Pleuromutilin is produced by Pleuromutilin ( Pleurotus mutilus ) is a class of broad-spectrum diterpene antibiotics produced by fermentation, and is the precursor of semi-synthetic derivatives of pleuromutilins. Pleuromutilins are a large family of antibiotics with good antibacterial activity, which can effectively inhibit most Gram-positive bacteria and some Gram-negative bacteria. [0003] At present, the basic raw materials of the seed medium of most domestic manufacturers of Pleurotus spp. are yeast powder, glucose, dextrin, potassium dihydrogen phosphate, and ammonium sulfate; the basic raw materials of the fermentation medium are soybean oil, glucose, hot-pressed soybean cake powder, and yeast powder. , potassium dihydrogen phosphate, mag...

Claims

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Application Information

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IPC IPC(8): C12P15/00C12R1/645
Inventor 王义任勇王文超纳卓斌奇乃
Owner 宁夏泰瑞制药股份有限公司
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