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Application of LECT2 protein in preparation of antiviral drugs

An antiviral drug, LECT2 technology, applied in antiviral agents, peptide/protein components, pharmaceutical formulations, etc., to achieve the effects of improving macrophage activity, inhibiting spleen enlargement, and inhibiting replication

Active Publication Date: 2014-03-12
NINGBO UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, LECT2 has not been found to have preventive and therapeutic effects on viruses

Method used

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  • Application of LECT2 protein in preparation of antiviral drugs
  • Application of LECT2 protein in preparation of antiviral drugs
  • Application of LECT2 protein in preparation of antiviral drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] 1.1 Materials: Influenza A virus strain (H1N1, A / Puerto Rico / 8 / 1934) was purchased from Zhejiang Academy of Medical Sciences, 9-day-old SPF (specific pathogen-free) chicken embryos were inoculated after virus recovery, and infected chicken embryos were harvested after 72 hours of culture The allantoic fluid was used to detect the hemagglutination titer of the virus, and the SPF chicken embryos were transferred to the 2nd to 3rd generations and stored at -70°C. The LECT2 protein is a recombinant LECT2 protein, and the preparation of the recombinant LECT2 protein can be carried out according to the methods published by Ovejero and others, which will not be described here.

[0018] 1.2 Recombinant LECT2 protein promotes IFN-γ secretion of macrophages

[0019] 1.2.1 Culture of lung macrophages: mice were anesthetized and fixed, the neck was shaved and disinfected, the neck was cut in the middle, and the trachea was exposed and stripped. Thread a slipknot at the proximal en...

Embodiment 2

[0024] Example 2 is basically the same as Example 1, except that the recombinant LECT2 protein is replaced by the LECT2 protein extracted directly from animals. Effect. The H1N1 virus can also be replaced by other Orthomyxoviridae members to obtain the above effects.

Embodiment 3

[0026] 2.1 LECT2 protein treatment inhibits mouse spleen enlargement caused by murine leukemia virus (MuLV, where Friend type is abbreviated as F-MLV) infection: F-MLV is quoted from the American Classical Culture Collection, and passed in vivo in mice to produce 10% spleen suspension, stored at -70°C. And determine the virus titer PFU. The mice were randomly divided into normal group (without injection of F-MLV+normal saline), control group (injection of F-MLV+normal saline), and experimental group (injection of F-MLV+LECT2), with 12 mice in each group Except for the normal group, the injection volume of F-MLV was 0.5ml of virus liquid (virus titer 100PFU) by intraperitoneal injection for each mouse. The experimental group was intraperitoneally injected with pure LECT2 protein (0.2 μg / g) 1 hour after F-MLV injection, and the normal group and control group were replaced with the same dose of normal saline for LECT2 protein. 30 days after the injection of F-MLV, the mice were...

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Abstract

The invention discloses an application of LECT2 protein in preparation of antiviral drugs, wherein the virus is orthomyxoviridae virus or retroviridae virus, the LECT2 protein can induce expression of antiviral cell factors, such as interferon Gamma and granulocyte colony stimulating factor, by activating the macrophage, so that macrophage activity is increased, animal immunity ability is enhanced, the reproduction of influenza virus and retroviridae virus is inhibited, splenauxe of F-MLV infected mice is inhibited, and the obvious effect of the livability of mice infected by influenza virus is improved and the side effects do not exist.

Description

technical field [0001] The present invention relates to the application of LECT2 protein, in particular to the application of LECT2 protein in the preparation of antiviral drugs. Background technique [0002] Leukocyte cell-derived chemotaxin 2 (LECT2 protein) is a multifunctional protein with a molecular weight of about 16kDa and three intramolecular disulfide bonds. The molecule has a simple structure and no glycosyl modification. LECT2 was initially considered as a neutrophil chemoattractant. With the deepening of research, it was found that it was also associated with bone and blood vessel growth, liver transplantation injury repair, plant toxins, non-pathogenic antigens, etc. Related to the process, for example, the publication number is CN102159238, titled Composition for Inhibiting Pathological Angiogenesis, which discloses a composition comprising an effective amount of LECT2 protein prepared to inhibit pathological angiogenesis and / or cell proliferation diseases. H...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/19A61P31/14A61P31/16
Inventor 陈炯陆新江史雨红虞朝辉
Owner NINGBO UNIV
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