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Polar-region and deep-sea habitat actinomycete having strong killing effect for various pests and metabolites thereof

A technology of metabolites and actinomycetes, applied in microorganisms, biochemical equipment and methods, bacteria, etc., to achieve the effect of good pesticide development potential, strong insecticidal activity and high content

Inactive Publication Date: 2012-09-19
田黎 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The indolecarbazole compound (Staurosporine) produced by marine organisms has been reported to be used against animal tumors, and no one has ever tried to kill insects

Method used

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  • Polar-region and deep-sea habitat actinomycete having strong killing effect for various pests and metabolites thereof
  • Polar-region and deep-sea habitat actinomycete having strong killing effect for various pests and metabolites thereof
  • Polar-region and deep-sea habitat actinomycete having strong killing effect for various pests and metabolites thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1: Streptomyces nitrosporus CQT14-24 bacterial strain is obtained, biological characteristic and identification

[0017] T14-24 Streptomyces nitrosporeus was isolated from the deep-sea sediments of the Chukchi Sea in the Arctic in August 2002. After being isolated, it was screened out by a conventional insecticidal model (Xiaocai's stomach toxicity model), and its biological and molecular biological characteristics Studies were conducted and strains were identified based on the above characteristics

[0018] Morphological characteristics: cultured in the modified Gaoshi No. 1 medium at 19°C for 7 days, and observed the morphology of the bacteria with an optical microscope.

[0019] Culture characteristics: After culturing in the above medium for 7 days, observe the colony formation and color.

[0020] Physiological and biochemical characteristics: refer to the "Handbook of Actinomycetes Identification" and use conventional methods.

[0021] Experimental res...

Embodiment 2

[0027] Embodiment 2: artificial cultivation and fermentation of Streptomyces nitrosporus CQT14-24 bacterial strain

[0028] After optimizing the growth conditions of the Streptomyces nitrosporus CQT14-24 bacterial strain by conventional methods, the most suitable medium was selected to be composed of the following: 12 g of soluble starch, 6 ml of corn infusion juice, 300 ml of sea mud infusion juice, KNO 3 1g, K 2 HPO 4 0.5g, MgSO 4 0.5g, NaCl 10g, KCl 0.03g, MgCl 2 0.23g, 700ml of water; the culture conditions of Streptomyces nitrosporusCQT14-24 strain are as follows: in a shaker flask or fermenter, the temperature is controlled between 19-23°C; the speed is controlled at 140-150rpm; , gas-liquid ratio and tank pressure can be controlled at 1:1 and 0.05MPa, pH 7.0-7.5, respectively.

[0029] During the cultivation process, defoamers such as soybean oil or foam enemy can be used for defoaming. The culture time is controlled between 14 and 15 days. At this time, the C...

Embodiment 3

[0030] Example 3: Fermentation Extraction and Metabolite Separation and Purification after Artificial Culture of Streptomyces nitrosporeus CQT14-24 Strain

[0031] The above fermented product is separated into supernatant and mycelium by conventional separation means such as centrifugation or filtration.

[0032] (1) Add acetone (volume 1:2) to extract the mycelium after breaking the wall, evaporate the extract to dryness, and obtain the crude acetone extract of the mycelium (abbreviation: acetone extract)

[0033] (2) The mycelium extract bacterial liquid is concentrated 5 times by conventional vacuum evaporation method, and it is a fermentation concentrated liquid (abbreviation: concentrated liquid)

[0034] (3) Add ethyl acetate (volume 1:1) to the fermentation concentrate for extraction, evaporate the extract to dryness, and obtain the ethyl acetate extract of the bacterial liquid (abbreviation: bacterial liquid extract)

[0035] The above crude extract was dissolved in 0.5...

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Abstract

The invention provides a polar-region and deep-sea habitat actinomycete having a strong killing effect for various pests and metabolites thereof. The actinomycete is characterized in that the actinomycete belongs to streptomyces nitrosporeus, CQT14-24, and the actinomycete is obtained by separating from sediments of the Chukchi Sea in the Arctic, with a preservation number of CGMCC No. 4607. Morphological characteristics of the actinomycete are that the front side of a bacterial colony is off-white, the back side of the bacterial colony is grey, the bacterial colony is not transparent, the bacterial colony is tightly combined with culture mediums, and spore mycelia are straight and soft, in clusters or clumps. Spores are in shapes from ellipse to oval, with smooth surface. Aerial mycelia are black gray, and substrate mycelia are light black gray. The application effects of the actinomycete lie in that fermentation broth bacteria liquid and mycelia of the CQT14-24 bacterial strain have strong insecticidal activity, obtained compound is only one of mycelia extracts, the mycelia extracts can be separated to gain other insecticidal compounds, and the bacterial strain has very good development potential for pesticides. Indolocarbazole compounds obtained from the bacterial strain has a high content in the fermentation broth and strong insecticidal activity, so that subsequent research works are easier to develop, and a novel pesticide is expected to be made in a short term.

Description

technical field [0001] The invention belongs to the technical field of marine microorganisms, and in particular relates to polar and deep-sea environment actinomycetes and metabolites that have strong killing effects on various pests. The strain is preserved in the General Microbiology Center of the China Committee for the Collection of Microbial Strains, the address is Datun Road, Chaoyang District, Beijing, the Institute of Microbiology, Chinese Academy of Sciences, the preservation date is February 25, 2011, the preservation number is CGMCC No.4607, and the classification name is Nitrogen Streptomyces nitrosporeus. Background technique [0002] Human agricultural production is always accompanied by the control of harmful organisms, and the long-term use of chemical pesticides has brought about side effects of environmental pollution. Therefore, solving the problem of chemical pesticide pollution in cultivated plants has always been a focus of worldwide attention. my coun...

Claims

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Application Information

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IPC IPC(8): C12N1/20C07D498/22A01P7/04C12R1/465
CPCY02A50/30
Inventor 田黎林文瀚史振平张久明
Owner 田黎
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