Method for producing bioprotein feed using tomato pomace
A technology of biological protein feed and tomato pomace, applied in animal feed, animal feed, application, etc., can solve the problems of immaturity and incomplete fermentation process, and achieve the effect of improving nutritional value
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example 1
[0021] (1) Strain and source
[0022] Candida utilis (Candida utilis), Geotrichum candidum, Saccharomyces cerevisiae, Aspergillus oryzae, Aspergillus fumigatus, strains were purchased from the China Industrial Microorganism Collection .
[0023] (2) Preparation of composite microorganisms
[0024] Original seed test tube activation-triangular flask expansion-seed tank culture-fermenter culture.
[0025] The described enzyme mother bacteria (Candida utilis, Geotrichum candidum, Saccharomyces cerevisiae) medium is 5°Bé wort medium: 100g of barley malt is crushed, put into a 1000ml beaker, 400-500ml of water is added, and cultured at 60°C at a constant temperature 4 to 5 hours, 8 layers of gauze filter. Add 20ml of water to an egg white, stir to a large amount of foam, pour it into the filtrate, boil, keep boiling for 5 minutes, filter with 8 layers of gauze to obtain clarified wort, measure the sugar content with a sugar meter, and dilute to 5°, 112.6°C Sterilize for 30 minutes and s...
example 2
[0036] (1) Strain and source: the same as in Example 1.
[0037] (2) Preparation of composite microorganisms: the suspensions of Candida utilis, Geotrichum candidum, Saccharomyces cerevisiae, Aspergillus oryzae and Aspergillus fumigatus were mixed at a ratio of 1:1:0.5:2.0:2.0 to obtain a composite microorganism suspension. Others are the same as in Example 1.
[0038] (3) Preparation of fermentation raw materials: fresh tomato residue accounted for 85%, bran in auxiliary materials accounted for 12%, urea 1.5% and ammonium nitrate 1.5%.
[0039] (4) Strain inoculation and preparation process
[0040] The composite microorganisms are converted into a solid mixed microbial agent by using sterilized wheat bran at an inoculum amount of 3% by weight of the raw material through conventional carrier technology.
[0041] After mixing the solid mixture with fresh tomato residue, ammonium nitrate and urea, solid fermentation is carried out. The solid fermentation stack height is 80cm, the fermen...
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