Method for preparing reagent-grade phycocyanin on large scale
A phycocyanin, reagent-grade technology, applied in the field of large-scale preparation of reagent-grade phycocyanin, can solve the problems of drinking water crisis, high price, low purity, etc., and achieve the effect of industrialization, low price and abundant sources
Active Publication Date: 2014-09-24
JIANGSU ACAD OF AGRI SCI
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Problems solved by technology
In 2007, thousands of meters of blue-green algae along the coast of Taihu Lake reached a thickness of about 10 cm, which made it impossible for Wuxi Gonghu Water Plant to supply water normally, triggering a drinking water crisis
The disadvantages of this method are low yield, low purity, high cost, and steps such as microfiltration, ultrafiltration, and column chromatography that are difficult to apply to large-scale production, making it difficult to realize industrialization of this method.
[0008] The supply of reagent grade phycocyanin is basically monopolized by foreign suppliers, and the price is extremely expensive
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preparation example Construction
[0019] The method for the large-scale preparation of reagent grade phycocyanin of specific implementation comprises the following steps:
[0020] The first step, freezing and thawing to break the wall: dehydrate the cyanobacteria to obtain algae mud with a dry matter content of 2-15% by mass; first freeze the algae mud at -35°C to -18°C, and then take it out and melt it , freeze and thaw at least twice;
[0021] The second step, flocculation extraction: dilute the frozen-thawed algae slurry with water, and mix to obtain the algae slurry, wherein the mass ratio of the algae slurry to water is 0.5-1.5:100; Aluminum chloride solution and mixed to obtain a mixed solution, wherein the mass ratio of polyaluminum chloride solution to algae pulp is 0.5-1.0:100; the mixed solution is allowed to stand for at least 12 hours to separate layers, and the blue supernatant is obtained to obtain algal blue Crude protein extract (can be taken out by slow pouring, siphoning, etc.);
[0022] Th...
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The invention relates to a method for preparing reagent-grade phycocyanin on large scale. The method comprises the following steps of: 1, freezing and thawing to break walls; 2, flocculating to extract; 3, salting out stepwise; 4, performing dialysis; and 5, purifying. According to the method, blue-green algae salvaged from lake are used as raw materials, and are abundant in source and low in price; the used reagents are commercially available products which are low in price; and the whole method is low in cost, the yield is high, and high-purity phycocyanin can be obtained. All the steps can be directly applied to large-scale production, and the industrialization can be realized conveniently.
Description
technical field [0001] The invention relates to a large-scale preparation method of reagent-grade phycocyanin, which belongs to the technical field of environmental protection and resource utilization. Background technique [0002] In recent years, the scale of cyanobacteria outbreaks in Taihu Lake has become larger and larger, the outbreak period has become longer and longer, and the damage caused has become higher and higher. In 2007, thousands of meters of blue-green algae along the coast of Taihu Lake reached a thickness of about 10 cm, which made it impossible for Wuxi Gonghu Water Plant to supply water normally, triggering a drinking water crisis. Mechanical and manual salvage has become an important emergency measure to prevent the deterioration of water quality caused by the outbreak of cyanobacteria in eutrophic lakes, but the salvage of cyanobacteria may cause secondary pollution and lead to new environmental problems. Taihu Lake salvages hundreds of thousands of ...
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IPC IPC(8): C07K14/795C07K1/36C07K1/34C07K1/30
Inventor 韩士群
Owner JIANGSU ACAD OF AGRI SCI