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Separating method for denitrifying bacteria

A denitrifying bacteria and separation method technology, applied in the field of denitrifying bacteria separation, can solve the problems of not effectively obtaining denitrifying bacteria, incapable of denitrifying bacteria for nitrification, denitrification, etc., to increase denitrification effect and increase impact resistance Effect

Inactive Publication Date: 2014-11-26
SOUNDGRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The embodiment of the present invention provides a method for separating denitrifying bacteria, which can solve the problem that there is no effective method for obtaining denitrifying bacteria at present, and the obtained denitrifying bacteria cannot be used for sewage treatment for nitrification and denitrification in the denitrification process.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] The present embodiment provides a kind of separation method of denitrifying bacteria, and this method is specifically as follows:

[0014] Raw material collection: collect the granular sludge solution in the upflow anaerobic sludge blanket (UASB) or the anoxic tank of the domestic sewage treatment plant as raw material, and use the vortex oscillator to crush the granular sludge in the granular sludge solution After making bacterial suspension;

[0015] Separation and screening culture: Separation and screening culture is carried out on the prepared bacterial suspension, and the culture is obtained after cultivation; specifically, the bacterial suspension is transferred into the separation and screening medium, and the pyrogallic acid method is used to obtain a culture medium at 25- The anaerobic culture is carried out at 35° C. for 5 to 7 days, and the culture is obtained after the culture.

[0016] The pyrogallic acid method adopted above is specifically: laying a lay...

Embodiment 2

[0033] The present embodiment provides a kind of separation method of denitrifying bacteria, and this method is specifically as follows:

[0034] Step 1, collect granular sludge solution as raw material: select the granular sludge solution in the anoxic tank of domestic sewage plant as the separation source;

[0035] Step 2, processing the raw material of the collected granular sludge solution, vibrating and crushing the granular sludge in the granular sludge solution with a vortex oscillator to make a bacterial suspension;

[0036] Step 3, separation and screening culture: transfer the bacterial suspension prepared in step 2 into the separation and screening medium, which can be coated on the separation and screening medium by means of coating; adopt the pyrogallic acid method, at a temperature of 25 to 35 ° C Under anaerobic culture for 10 to 15 days, the isolated and screened cultures were obtained after cultivation;

[0037] The separation and screening medium in the abov...

Embodiment 3

[0049] The present embodiment provides a kind of separation method of denitrifying bacteria, and this method is specifically as follows:

[0050] Step 1, collect granular sludge solution as raw material: select the sludge solution in the upflow anaerobic sludge blanket (UASB) as the separation source;

[0051] Step 2, processing the collected raw materials, vibrating and crushing the granular sludge in the sludge solution with a vortex oscillator to make a bacterial suspension;

[0052] Step 3, separation and screening culture: transfer the bacterial suspension prepared in step 2 into the separation and screening medium, use the pyrogallic acid method, and perform anaerobic culture at a temperature of 25 to 35°C for 10 to 15 days, and obtain Cultures;

[0053] The separation and screening medium in the above step 3 is composed of the following raw materials: distilled water 1000ml, sodium citrate 4g-6g, potassium nitrate 1.7g-2.3g, dipotassium hydrogen phosphate 0.8g-1.2g, po...

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Abstract

The invention discloses a separating method for denitrifying bacteria, and belongs to the field of bacteria separation. The method comprises the following steps of: collecting a raw material: collecting a granular sludge solution serving as the raw material, and preparing bacterium suspension from the granular sludge solution; separating, screening and culturing: separating, screening and culturing the prepared bacterium suspension to obtain a cultured substance; and detecting the cultured substance: detecting the cultured substance obtained by separating, screening and culturing, wherein bacteria in the cultured substance passing the detection are the denitrifying bacteria. The separating method has the advantages of simplicity and quick separation of the denitrifying bacteria, can provide the pure cultured substance of the denitrifying bacteria, and provide assistance for microbiological theoretical research and industrialized application.

Description

technical field [0001] The invention relates to the field of bacteria separation, in particular to a method for separating denitrifying bacteria. Background technique [0002] Denitrifiers are facultative anaerobic microorganisms that convert nitrate nitrogen into nitrogen gas. Such microorganisms can generally induce nitrate reductase A and nitrite reductase required for denitrification only under anaerobic conditions, and use nitrate or nitrite as the electron acceptor for denitrification. Due to the different environments required by microorganisms, in the whole denitrification process of sewage treatment, that is, the nitrification process and denitrification process, the impact resistance of the system is relatively poor. High concentrations of ammonia nitrogen and nitrate nitrogen will inhibit the growth of nitrifying bacteria. The concentration of dissolved oxygen and insufficient carbon source will inhibit the growth of denitrifying bacteria, thus affecting the over...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/02
Inventor 屈佳玉
Owner SOUNDGRP CO LTD