Preparation method of cell-loaded biological microcapsules
A microcapsule and biological technology, applied in the fields of fixed on/in organic carriers, medical science, prosthesis, etc., can solve problems such as size discomfort, high leakage, high risk, etc.
Inactive Publication Date: 2014-10-08
SOUTHEAST UNIV
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Problems solved by technology
The materials used in the present invention are cheap and easy to obtain, and the new technology involved has a simple route and high technical content, and the product contains a large amount of cells, has good isolation and self-degradation performance, and is non-toxic to cells and organisms, and is expected to replace the currently used The traditional materials and preparation methods solve the problems of different sizes, difficulty in hollowing, improper size, high leakage and high risk in the use of similar materials in the preparation process of capsules
Method used
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Embodiment 1
[0018] 1. Degradation treatment of cationic polyelectrolyte polysaccharides such as gelatin, polyglucosamine, hydroxymethyl chitosan, chitosan and chitooligosaccharide, taking hydroxymethyl chitosan, chitooligosaccharide and gelatin as examples:
[0019] Preparation of low molecular weight hydroxymethyl chitosan with hydroxymethyl chitosan:
[0020] The reaction is carried out in a heterogeneous system. Hydroxymethyl chitosan is first dispersed in deionized water, then hydrogen peroxide is added at a mass ratio of 1:1, and the degradation reaction is carried out in a constant temperature water bath. The reaction time is 8-10 hours to control The molecular weight of the product, the steps of the specific degradation reaction are as follows: After installing the experimental equipment according to the experimental requirements, add the weighed (3.000g) hydroxymethyl chitosan, and then add deionized water at a mass ratio of 25:1 (W:W) 75g, add 78g of hydrogen peroxide, fully disp...
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Abstract
The preparation method of the cell-loaded biological microcapsules, the preparation steps are: a. mixing an anionic surfactant with an oil phase to prepare a water-in-oil type inverse emulsion system, and mixing the oil phase with an anionic surfactant to obtain a water-in-oil emulsion system type inverse emulsion system, so that the mixed solution is milky white, and it is not delaminated at room temperature for 30 minutes; b. degrade the cationic polyelectrolyte polysaccharide to obtain a cationic polyelectrolyte polysaccharide with a molecular weight of two thirds before degradation; c. Adding the low-molecular-weight cationic polyelectrolyte polysaccharide after the degradation treatment in step b to the water-in-oil inverse emulsion system prepared in step a, polymerizing into capsules through self-assembly reaction; d. separating the solid-liquid separation of the capsule-containing emulsion system obtained in step c , to obtain solid microcapsules.
Description
Technical field [0001] The present invention is an auxiliary material and preparation technology field, and specially involves a method for preparing cell biochemicals. Background technique [0002] For tissue engineering, it has been found that stem cells or related cells can effectively treat bone necrosis through the surface where the vectors are implanted into the wicked bone. For biological engineeringLocal medication; for clinical applications, granular loading cells are more likely to perform surgery to ensure the activity of cells during treatment. [0003] Making non -immune -inhibiting natural products into microcapsules can solve many problems facing tissue engineering, biological engineering and clinical applications, and have certain positive significance for the biomedical field.As a natural product of non -immune suppression, due to its superior performance, natural polysaccharides have always been the object of academic research. In the past ten years, a large num...
Claims
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IPC IPC(8): C12N11/10C12N11/04A61L27/50A61L27/54
Inventor 王婷冯章启何农跃
Owner SOUTHEAST UNIV