Solid synchronous enzymolysis fermentation method for lignocellulose airborne ethanol particles packed bed

A lignocellulose, particle packed bed technology, applied in fermentation, biofuel and other directions, can solve the problems of low ethanol yield and strong product inhibition, achieve high fermentation substrate concentration and product yield, operation and extraction Simple and controllable process, low investment and operating costs

Inactive Publication Date: 2012-10-03
CHONGQING UNIV
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  • Abstract
  • Description
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Problems solved by technology

At present, the second and third steps are carried out in different reactors, that is, the saccharification process is carried out first, and the monosaccharides produced are

Method used

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  • Solid synchronous enzymolysis fermentation method for lignocellulose airborne ethanol particles packed bed

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Embodiment 1

[0022] A solid-state synchronous enzymatic hydrolysis fermentation method for lignocellulose airborne ethanol particles packed bed, the method comprises the following steps:

[0023] A. Making embedding granules: Cellulase, cellobiase, xylosidase, and straw with a particle size of 40-80 meshes are made of polyvinyl alcohol and sodium alginate to form embedding granules. The method of making embedding granules is as follows: Weigh a certain amount of sodium alginate and polyvinyl alcohol into a beaker with an appropriate amount of water, heat and dissolve, then cool it to room temperature, add cellulase, cellobiase and xylase, mix well, and then mold It is made into embedding particles, and the particle size of the embedding particles is 2-6mm; then the embedding particles are put into 2 Cross-link in a saturated boric acid solution at 4-5°C for 12-24 hours; then wash off the boric acid and calcium chloride (CaCl) on the surface of the embedded particles 2 ) solution, as a spa...

Embodiment 2

[0029] The present invention will be described in further detail below in conjunction with the accompanying drawings and specific embodiments.

[0030] A solid-state synchronous enzymatic hydrolysis fermentation method for lignocellulose airborne ethanol particles packed bed, the method comprises the following steps:

[0031] A. Making embedding granules: Cellulase, cellobiase, xylosidase, and straw with a particle size of 40-80 meshes are made of polyvinyl alcohol and sodium alginate to form embedding granules. The method of making embedding granules is as follows: Weigh a certain amount of sodium alginate and polyvinyl alcohol into a beaker with an appropriate amount of water, heat and dissolve, then cool it to room temperature, add cellulase, cellobiase and xylose, mix well, and then mold It is made into embedding particles, and the particle size of the embedding particles is 2-6mm; then the embedding particles are put into 2 Cross-link in a saturated boric acid solution a...

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Abstract

The invention discloses a solid synchronous enzymolysis fermentation method for a lignocellulose airborne ethanol particles packed bed, which comprises the following steps: A) manufacturing embedded particles; B) installing a system of a packed bed reactor and filling the embedded particles; C) uniformly dripping yeast liquid onto the surfaces of the embedded particles so as to form a biomembrane, and utilizing glucose generated from the internal reaction of the embedded particles to generate ethanol and carbon dioxide by the yeast biomembrane under an anaerobic condition; and D) introducing carrier gas from the bottom of the reactor, causing the carrier gas to uniformly and upward flow by the embedded particles packed bed, timely taking the generated ethanol and carbon dioxide out of the reactor, entering into a gas bottle and also taking away the metabolic heat generated by the reaction. A solid synchronous diastatic fermentation mode is adopted by the invention, so that the glucose generated during a saccharifying process is quickly converted into the ethanol by yeast under the anaerobic condition; the carrier gas is introduced into the reactor during the fermentation process, so that the product inhibition is eliminated; the ethanol is taken away while local metabolic heat is also taken away; and the yield of the ethanol is increased.

Description

technical field [0001] The invention relates to a method for producing ethanol, in particular to a solid-state synchronous enzymolysis fermentation method for a packed bed of lignocellulose airborne ethanol particles. Background technique [0002] As a green renewable resource, cellulosic ethanol can replace non-renewable petroleum resources. It has a wide range of applications. Under the conditions of shortage of petroleum resources in the world, rising crude oil prices, and increasingly severe environmental pressure, cellulosic ethanol has broad market prospects and social benefit. However, due to the high cost of cellulosic ethanol production, low ethanol yield, and the production technology is still immature, it is of great significance to study efficient cellulosic ethanol production technology in order to increase ethanol yield and reduce costs. [0003] At present, there are two main fermentation methods for cellulosic ethanol. One is liquid fermentation. Liquid ferm...

Claims

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Application Information

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IPC IPC(8): C12P7/10
CPCY02E50/16Y02E50/10
Inventor 王永忠王艳梅朱恂廖强陈蓉李俊叶丁丁周劲
Owner CHONGQING UNIV
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