Preparation method for manufacturing silkworm pupa protein of protein filament

A technology of silkworm chrysalis protein and silkworm chrysalis protein powder, which is applied to the preparation method of peptides, chemical instruments and methods, peptides, etc. It can solve the problems of broken silkworm chrysalis protein fibers, recycling of silkworm chrysalis protein peptides, and affecting the quality of spinning, and achieves the goal of product The effect of high quality, low production cost, and convenience for industrial scale production

Inactive Publication Date: 2012-10-10
成都起点投资有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main disadvantages of this method are: microwave treatment cannot break the chemical connection between globulin and chitin, and the extracted silkworm chrysalis protein is a compound of silkworm chrysalis protein and chitin, which may cause allergic reactions when used as food additives; silkworm chrysalis prepared with it The protein alkali solution is peptone at room temperature, which blocks the spinning hole, resulting in the

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A method for preparing silkworm chrysalis protein for making protein silk, the specific steps of the method are as follows:

[0026] (1) Preparation of defatted silkworm chrysalis powder alkaline solution

[0027] Using commercially available degreased silkworm chrysalis as raw material, the degreased silkworm chrysalis is first sent into a pulverizer for pulverization, then the powder is collected and separated through a 20-mesh sieve, and the sieved powder (that is, the prepared degreased silkworm chrysalis powder) is collected; The crude product is returned to the pulverizer for further pulverization. The defatted silkworm chrysalis powder that collects is sent in the batching kettle again, according to the quality (kg) of defatted silkworm chrysalis powder: the massfraction of sodium hydroxide is that the ratio of the volume (L) of the aqueous solution of 0.1% sodium hydroxide is 1: 8 Ratio, pump the sodium hydroxide aqueous solution into the batching kettle, stir ...

Embodiment 2

[0037] A preparation method of silkworm chrysalis protein for making protein silk, same as embodiment 1, wherein:

[0038] In the (1) step, collect powder and cross 40 mesh sieves to separate, according to the quality (kg) of defatted silkworm chrysalis powder: the massfraction of sodium hydroxide is the ratio of the volume (L) of the aqueous solution of 1% sodium hydroxide is The ratio of 1:12, pump the sodium hydroxide aqueous solution into the batching kettle.

[0039] In step (2), water vapor at a pressure of 0.25 MPa is introduced into the interlayer of the glass-lined reactor to raise the temperature of the defatted silkworm chrysalis powder alkaline solution in the glass-lined reactor to 75° C. and keep it warm for 0.75 h. Continue to feed water vapor under stirring and continue to raise the temperature to 107 ° C, and keep warm for 1 hour before turning off the water vapor. Then, under stirring, the temperature of the mixed solution of the broken silkworm chrysalis gl...

Embodiment 3

[0044] A preparation method of silkworm chrysalis protein for making protein silk, same as embodiment 1, wherein:

[0045] In the (1) step, collect powder and cross 60 mesh sieves to separate, according to the quality (kg) of defatted silkworm chrysalis powder: the massfraction of sodium hydroxide is the ratio of the volume (L) of the aqueous solution of 1% sodium hydroxide is The ratio of 1:16, pump the sodium hydroxide aqueous solution into the batching tank.

[0046] In step (2), water vapor with a pressure of 0.4 MPa is introduced into the interlayer of the glass-lined reactor to raise the temperature of the defatted silkworm chrysalis powder alkaline solution in the glass-lined reactor to 80° C. and keep it warm for 1 hour. Continue to feed water vapor under stirring and continue to raise the temperature to 115°C, and keep the temperature for 1.5h again, then turn off the water vapor. Then, under stirring, the temperature of the mixed solution of the broken silkworm chry...

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Abstract

The invention relates to a preparation method for manufacturing silkworm pupa protein of protein filaments, relating to a method for extraction of silkworm pupa protein from silkworm pupa. The defatted silkworm pupa available commercially is used as a raw material, and the product is obtained via preparation of alkaline solution of defatted silkworm pupa powder, chitin-free silkworm pupa protein solution and precipitation solution thereof and silkworm pupa protein powder for filaments and recycling of silkworm pupa protein peptide. The method is simple, the production equipment is conventional general equipment, the energy consumption is low, the cost is low, and the promotion and use can be facilitated; and high-quality silkworm pupa protein, silkworm pupa protein peptide and other products can be produced according to the method disclosed by the invention, the silkworm pupa can be comprehensively and fully utilized, no ''three-waste'' emissions can be produced, the environmental protection can be facilitated, and the method belongs to a preparation method of green bio-chemical products. The method can be widely applied in preparation of silkworm pupa protein and silkworm pupa protein peptide. The silkworm pupa protein prepared in the method can be applied in manufacturing of adhesive protein filaments, food additives, bio-chemical culture mediums for fermentation, feed additives and the like; and the prepared silkworm pupa protein peptide can also be used as a high-quality nutritional agent for animal cubs.

Description

1. Technical field [0001] The invention belongs to the technical field of animal protein preparation. It specifically relates to a method for extracting silkworm chrysalis protein from silkworm chrysalis. 2. Background technology [0002] Silkworm chrysalis is the main by-product of the silk reeling industry, and nearly 1 ton of dried silkworm chrysalis can be produced for every 1 ton of raw silk produced. China is the most important silk producer in the world. According to statistics, my country can produce more than 200,000 tons of dried silkworm chrysalis by-products every year. According to measurements, dried silkworm chrysalis contains about 48% to 60% of crude protein, 25% to 31% of fatty acid, 2% to 5% of sugar, and 2% to 3% of chitin. Silkworm chrysalis protein is a high-quality pure natural full-valent protein. Its efficacy ratio and biological value are significantly better than soybean protein, and it is easy to be digested and absorbed by the human body, and i...

Claims

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Application Information

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IPC IPC(8): C07K1/36C07K1/34C07K1/30C07K1/14
Inventor 孙烽周小华余文琴蔡怀德冯海燕
Owner 成都起点投资有限公司
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