Dead lactobacillus biomass for antimicrobial use and a production method therefor

A technology of lactic acid bacteria and antibacterial substances, applied in the directions of biochemical equipment and methods, medical raw materials derived from bacteria, medical preparations containing active ingredients, etc. Excellent antibacterial activity, the effect of inhibiting the growth of bacteria

Active Publication Date: 2012-10-10
CELL BIOTECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the product is not easy to have a uniform appearance, and the antibacterial activity will also vary according to the number of dead lactic acid bacteria in the heat-inactivated and freeze-dried materials

Method used

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  • Dead lactobacillus biomass for antimicrobial use and a production method therefor
  • Dead lactobacillus biomass for antimicrobial use and a production method therefor
  • Dead lactobacillus biomass for antimicrobial use and a production method therefor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0082] Such as Figure 5 As shown, the method for preparing heat-inactivated dead lactic acid bacteria freeze-dried product of the present invention is adopted.

[0083] Specifically, the complete fermentation broth was separated into a fermentation filtrate and a solution containing lactic acid bacteria using a centrifuge at 7,260 RPM. Transfer the fermentation filtrate to a pressure vessel (fermenter), heat inactivate at 90°C for 10 minutes, cool to 40°C, repeat heat inactivation at 110°C for 10 minutes, cool to 40°C, and use a fluidized bed dryer A 2-fold (2X) concentration using soy protein isolate and powdered skim milk as absorbents yields a heat-inactivated and dried fermentation filtrate.

[0084] Mix the solution containing lactic acid bacteria, dilute it with purified water at a ratio of 1:2, and heat inactivate it at 121°C for 20 minutes in a steam autoclave. Based on the solution containing heat-inactivated dead lactic acid bacteria, use 20% by weight of Trehalos...

Embodiment 2

[0086] Such as figure 2 As shown, the method for preparing heat-inactivated dead lactic acid bacteria freeze-dried product of the present invention is adopted.

[0087] The complete fermentation broth of lactic acid bacteria is heat-inactivated at 90°C for 10 minutes, cooled to 40°C, repeated heat-inactivated at 110°C for 10 minutes, cooled to 40°C, and then separated into lactic acid bacteria containing heat-killed dead lactic acid bacteria by centrifuge solution and heat-inactivated fermentation filtrate. Then, the heat-inactivated fermentation filtrate was concentrated twice (2×) by using a fluidized bed dryer, and soybean protein isolate and powdered skim milk were used as absorbents, thereby obtaining a heat-inactivated and dried fermentation filtrate.

[0088] Use trehalose and starch based on 20% by weight of the heat-inactivated dead lactic acid bacteria solution as a cryoprotectant at room temperature to coat the solution containing heat-inactivated dead lactic acid...

Embodiment 3

[0090] Such as image 3 As shown, the method for preparing heat-inactivated dead lactic acid bacteria freeze-dried product of the present invention is adopted.

[0091] Heat inactivate the complete fermentation broth of lactic acid bacteria at 90°C for 10 minutes, cool to 40°C, repeat heat inactivation at 110°C for 10 minutes, cool to 40°C, and use a centrifuge to separate it into heat-inactivated dead lactic acid bacteria solution and heat-inactivated fermentation filtrate. Then, using an evaporator (concentrator) to carry out 5 times (5×) concentration of the separated heat-inactivated fermentation filtrate, using 20% ​​by weight of soybean protein isolate and powdered skimmed milk based on the heat-inactivated fermentation filtrate as absorbents, Freezing in a freezer at -40°C or below for 48 hours, freeze-drying in a freeze dryer for 96 hours, and grinding, thereby preparing a heat-inactivated and dried fermentation filtrate.

[0092] Use trehalose and starch based on 20...

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Abstract

The present invention relates to a dead lactobacillus biomass for antimicrobial use; the biomass being produced by subjecting a lactobacillus fermentation liquid to tyndallisation and then separating the same into a fermentation filtrate and a biomass-containing liquid, subjecting the biomass-containing liquid to freeze drying, and subjecting the fermentation filtrate to either fluidised-bed drying or freeze drying, and then mixing the fermentation filtrate and the biomass resulting from the drying processing. The present invention also relate to a production method for the dead lactobacillus biomass. The dead lactobacillus biomass for antimicrobial use according to the present invention has the advantage that it has a uniform composition and exhibits outstanding antimicrobial activity. Consequently, the same can be used to advantage during the production of various medical products, foods, animal feeds and cosmetics where antimicrobial activity and a bowel-regulating action are required.

Description

technical field [0001] The present invention relates to an antibacterial substance comprising heat-inactivated (tyndallized) dead lactic acid bacteria lyophilizate and dried heat-inactivated fermentation filtrate, more specifically, an antibacterial substance comprising heat-inactivated dead lactic acid bacteria lyophilized substance, The heat-inactivated dead lactic acid bacteria freeze-dried product has a uniform appearance and excellent antibacterial activity, and its preparation includes: a step of indirect heating at an appropriate temperature and flow rate in a heat exchanger for rapid heat inactivation (tyndallization) cooling step, fluidized bed drying step and freeze drying step; the present invention also relates to the preparation method of said antibacterial substance. Background technique [0002] Generally, live lactic acid bacteria exist in the intestinal tract and have various physiological activities, such as stimulating intestinal motility, inhibiting harmf...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12Q1/02A61K35/74A23K1/17A23K20/195A61K35/744A61K35/747
CPCA23K1/008A61K35/747C12Q1/02A61K35/74A23K1/17A61K35/744A23K1/002C12N1/20A23K10/16A23K40/10C12N1/04
Inventor 郑明俊
Owner CELL BIOTECH
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