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Feed additive composed of cellulose and sodium bicarbonate, feed and applications of feed additive and feed, as well as chicken coccidiosis live oocyst vaccine

A feed additive and cellulase technology, which is applied in the field of chicken coccidiosis live oocyst vaccine, can solve the problems of difficult removal of solids, and achieve the effect of reducing the content

Inactive Publication Date: 2012-10-17
烟台青大生物制药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the solids are relatively close to the vaccine oocysts in terms of specific gravity and size, it is not easy to remove all the solids in the prior art. How to further remove the solids in the vaccine liquid is a problem that needs to be solved

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Two feeds were formulated. 75 kg of corn, 20 kg of soybean meal, 5 kg of premix, mixed evenly, this is feed A. The formula of feed B is the same as that of feed A, but additionally, cellulase is added in an amount of 200U per kilogram of feed, and an additional amount of sodium bicarbonate is added in an amount of 10 grams per kilogram of feed.

[0029] 1. Feeding and vaccination. 200 21-day-old laying hens, half male and half male, were divided into two groups, A and B. Group A was fed feed A, and group B was fed feed B. Eat ad libitum. Inoculate 50,000 sporulated oocysts of the attenuated Eimeria muriformis strain to each chicken in groups A and B.

[0030] 2. Separation of feces and oocysts. (1) 96 hours after inoculation, collect 1000 grams of feces from chickens in groups A and B. (2) Filter the collected chicken manure A and B through a 60-mesh sieve to obtain 10,000 ml of filtrate each. (3) Centrifuge the filtrates of A and B to obtain A and B precipitates...

Embodiment 2

[0036] Two feeds were formulated. 75 kg of corn, 18 kg of soybean meal, 2 kg of fish meal, 5 kg of premix, mixed evenly, this is feed A. The formula of feed B is the same as that of feed A, but additionally added cellulase and sodium bicarbonate, the addition amount is 1000U per kilogram of feed cellulase, and the amount of sodium bicarbonate is 50 grams per kilogram of feed.

[0037] 1. Feeding and vaccination. 200 21-day-old laying hens, half male and half male, were divided into two groups, A and B. Group A was fed feed A, and group B was fed feed B. Eat ad libitum. Inoculate 50,000 sporulated oocysts of the attenuated Eimeria muriformis strain to each chicken in groups A and B.

[0038] 2. Separation of feces and oocysts. (1) 96 hours after inoculation, collect 1000 grams of feces from chickens in groups A and B. (2) Filter the collected chicken manure A and B through a 60-mesh sieve to obtain 10,000 ml of filtrate each. (3) Centrifuge the filtrates of A and B to ob...

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PUM

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Abstract

The invention discloses a feed additive composed of cellulose and sodium bicarbonate, a feed and applications of the feed additive and the feed, as well as a chicken coccidiosis live oocyst vaccine. The feed additive is composed of cellulose and sodium bicarbonate. An application of the cellulose and sodium bicarbonate in the reduction of the solid content in a vaccine liquid during the preparation of the chicken coccidiosis live oocyst vaccine is provided. An application of the cellulose and sodium bicarbonate in the feed in the reduction of the solid content in the vaccine liquid during the preparation of the chicken coccidiosis live oocyst vaccine is provided. A feed applied to the preparation of the chicken coccidiosis live oocyst vaccine is provided. An application of the feed in the reduction of the solid content in the vaccine liquid during the preparation of the chicken coccidiosis live oocyst vaccine is provided. The chicken coccidiosis live oocyst vaccine is manufactured by the following steps of: preparing the feed; adding the cellulose and sodium bicarbonate in the normal chicken feed; feeding and inoculating; collecting manure and separating oocyst. The beneficial effects of the invention are that the solid content in the vaccine can be reduced by adding the cellulose and sodium bicarbonate.

Description

[0001] technical field [0002] The invention relates to a feed additive composed of cellulase and sodium bicarbonate, a feed and its application, and a chicken coccidiosis live oocyst vaccine. Background technique [0003] Chicken coccidiosis is a protozoan parasitic disease caused by Eimeria tenella, Eimeria mumps, Eimeria giant, Eimeria poisoning, Eimeria lentis, Caused by one or more of Eimeria brucei and Eimeria precociousness. They are parasitic in various parts of the intestines of chickens, causing intestinal inflammation, hemorrhage and death of chickens, causing huge economic losses to the chicken industry. [0004] For a long time, the prevention and treatment of the disease has been dominated by drugs. Because drug residues affect food safety and human health, and coccidia are prone to drug resistance, the prevention and treatment of the disease increasingly relies on immunization. [0005] The chicken coccidiosis vaccines currently in use are mainly live oocy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/175A23K1/18A61K39/012A61P33/02
Inventor 郝言芝王江青
Owner 烟台青大生物制药有限公司
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