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Poly(DL-lactic acid) material modified based on 24 peptides in E domain of mechano-growth factor (MGF) and diamine, and preparation method and application thereof

A growth factor and domain technology, applied in the direction of prosthesis, medical science, etc., can solve the problems such as no other reports, achieve good hydrophilicity, cell affinity and biodegradability, and improve the effect of stress deficiency

Inactive Publication Date: 2012-10-17
CHONGQING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Aiming at the above problems, the present invention utilizes 24-peptide-modified diamine and maleic anhydride-modified polylactic acid to prepare a novel bionic scaffold and medical material for tissue engineering, but no other reports have been seen yet.

Method used

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  • Poly(DL-lactic acid) material modified based on 24 peptides in E domain of mechano-growth factor (MGF) and diamine, and preparation method and application thereof
  • Poly(DL-lactic acid) material modified based on 24 peptides in E domain of mechano-growth factor (MGF) and diamine, and preparation method and application thereof
  • Poly(DL-lactic acid) material modified based on 24 peptides in E domain of mechano-growth factor (MGF) and diamine, and preparation method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The preparation of embodiment 1 maleic anhydride modified polylactic acid

[0037] Mix 5.0 g of poly-D, L-lactic acid, 0.5 g of maleic anhydride, and 10 mg of tert-butyl peroxide evenly, and vacuum melt and seal it in a 50 ml round bottom flask. React at 50°C for 24 hours to obtain brown-yellow maleic anhydride-modified polylactic acid. The product was dissolved in dichloromethane and dropped into excess ether, and the precipitate was collected. Repeat to dissolve the pellet three times. The precipitate was placed under vacuum at room temperature for 48 hours to obtain 3.8 grams of brown maleic anhydride-modified polylactic acid, the molecular formula of which is as follows: Wherein A is D, L-lactic acid. In addition, through parallel tests: when A is D-lactic acid or L-lactic acid, it can all be realized.

Embodiment 2

[0038] The preparation of embodiment 2 maleic anhydride modified polylactic acid

[0039] 5.0 g of poly(D,L-lactic acid), 0.5 g of maleic anhydride, and 20 mg of tert-butyl peroxide were mixed evenly, and vacuum melted and sealed in a 50 ml round bottom flask. React at 50°C for 24 hours to obtain brown-yellow maleic anhydride-modified polylactic acid. The product was dissolved in dichloromethane and dropped into excess ether, and the precipitate was collected. Repeat to dissolve the pellet three times. The precipitate was placed under vacuum for 48 hours at room temperature to obtain 3.5 grams of brown maleic anhydride-modified polylactic acid, the molecular formula of which is Wherein A is D, L-lactic acid. In addition, through parallel tests: when A is D-lactic acid or L-lactic acid, it can all be realized.

Embodiment 3

[0040] The preparation of embodiment 3 maleic anhydride modified polylactic acid

[0041] 5.0 g of poly(D,L-lactic acid), 0.5 g of maleic anhydride, and 30 mg of tert-butyl peroxide were uniformly mixed, vacuum melted and sealed in a 50 ml round bottom flask. React at 50°C for 24 hours to obtain brown-yellow maleic anhydride-modified polylactic acid. The product was dissolved in dichloromethane and dropped into excess ether, and the precipitate was collected. Repeat to dissolve the pellet three times. The precipitate was placed under vacuum at room temperature for 48 hours to obtain 4.2 g of brown maleic anhydride-modified polylactic acid, the molecular formula of which is as follows: Wherein A is D, L-lactic acid. In addition, through parallel tests: when A is D-lactic acid or L-lactic acid, it can all be realized.

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Abstract

The invention relates to a poly(DL-lactic acid) material modified based on 24 peptides in E domain of MGF and diamine. The molecular formula of the poly(DL-lactic acid) material is represented by I. A preparation method for the poly(DL-lactic acid) material is as follows: diamine reacts with maleic anhydride modified poly(DL-lactic acid) to produce diamine-maleic anhydride modified poly(DL-lactic acid); diamine-maleic anhydride modified poly(DL-lactic acid) and the 24 peptides in the E domain of MGF are used as raw materials, one selected from the group consisting of DCC, EDC and a mixture is used as a condensing agent, then the raw materials and the condensing agent are subjected to a reaction at a temperature of 0 to 50 DEG C for 8 to 48 h, an obtained reaction solution is added into an excess aqueous medium, a membrane-like precipitate is collected, and the membrane-like precipitate is the poly(DL-lactic acid) material modified based on the 24 peptides in the E domain of MGF and diamine. The invention also discloses application of the poly(DL-lactic acid) material modified based on the 24 peptides in the E domain of MGF and diamine in preparation of bioactive bionic materials. The material provided in the invention has better hydrophilicity, cellular affinity and biodegradability and is expected to mitigate related problems caused by understressing during the process of tissue repair or regeneration treatment.

Description

technical field [0001] The invention belongs to the field of biological materials, in particular to polypeptide modified polylactic acid materials. Background technique [0002] Growth factor (mechano growth factor, MGF) is an alternative splicing variant of growth factor-1 (IGF-1), which is sensitive to stress stimuli. higher expression. Further research found that the MGF carboxy-terminal E domain 24 peptide (tyrosine-glutamine-proline-proline-serine-threonine-asparagine-lysine-asparagine-threonine - Lysine - Serine - Glutamine - Arginine - Arginine - Lysine - Glycine - Serine - Threonine - Phenylalanine - Glutamic Acid - Glutamic Acid - Histidine - Lysine Acid) has the function of promoting bone defect repair. However, a single polypeptide cannot achieve the desired effect in bone defect repair because of its short half-life in vivo. Therefore, a stable, economical and reliable delivery bracket system is particularly important. In the past decade, various materials ha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08G63/91C08G63/08A61L27/22A61L27/50
Inventor 王远亮李玉筱张兵兵潘君王品品
Owner CHONGQING UNIV
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