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Nanoparticles used for biological detection with fluoride lutecium as main component

A nanoparticle and biological detection technology, applied in material excitation analysis, fluorescence/phosphorescence, etc.

Inactive Publication Date: 2012-10-17
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the present inventors have found that despite their imaging applications, these materials have considerable limitations in biological (imaging) detection

Method used

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  • Nanoparticles used for biological detection with fluoride lutecium as main component
  • Nanoparticles used for biological detection with fluoride lutecium as main component
  • Nanoparticles used for biological detection with fluoride lutecium as main component

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] Example 1: NaXF 4 (X=79mol%Lu, 20mol%Yb, 1mol%Tm) Preparation of mixed nanoparticles

[0070] Salt mixed in proportion (CF 3 COO) 3X (X=79mol%Lu, 20mol%Yb, 1mol%Tm) totals 2 mmol, add 4 mmol of sodium trifluoroacetate, disperse in 20ml oleylamine, heat up to 110°C to remove water for 1 hour, then heat up to 340°C, Keep the temperature for 2 hours, then cool to room temperature, add 30 ml of cyclohexane, disperse with KQ-500DB ultrasonic generator at 80% power for 5 minutes, then centrifuge at 14000r / min for 10 minutes, discard the liquid part, and wash the precipitated solid with cyclohexane Wash 3 times (10 ml each time). The washed solid was vacuum dried at room temperature for 12 hours to obtain NaXF 4 (X=79mol%Lu, 20mol%Yb, 1mol%Tm) mixed nanoparticles. identified, such as figure 1 As shown in b, the particle size of the nanoparticle is about 20nm, and the particle size is basically uniform, indicating that practical nanoscale particles have been prepar...

Embodiment 2

[0073] Example 2: LiXF 4 (X=88mol%Lu, 10mol%Yb, 1.6mol%Er, 0.4mol%Tm) Preparation of mixed nanoparticles

[0074] Rare earth chloride salt XCl mixed in proportion 3 (X=88mol%Lu, 10mol%Yb, 1.6mol%Er, 0.4mol%Tm) total 1mmol, dispersed in the mixed solution of 10mL oleic acid and 10mL octadecene, heated to 160°C to form a uniform solution, cooled to room temperature, add 10 mL of NH containing 2 mmol LiOH and 4 mmol 4 The methanol solution of F was stirred for 30 min, heated to 100 °C to remove methanol for 1 h, then heated to 300 °C under the protection of Ar gas for 1 h, cooled to room temperature, added 25 ml of toluene, centrifuged at 12000 rpm for 8 min, discarded the liquid part, and used Disperse and wash with toluene and centrifuge 3 times (suspend the precipitate with 10 ml of toluene each time, then centrifuge at 12000 rpm for 5 min, discard the liquid part), and dry the precipitated solid in vacuum at room temperature for 24 hours to obtain LiXF 4 (X = 88mol...

Embodiment 3

[0077] Example 3: KXF 4 (X=65mol%Lu, 30mol%Yb, 5%molDy) Preparation of mixed nanoparticles

[0078] Mix 1.2g KOH, 9ml water, 10ml ethanol and 20ml oleic acid together, stir to obtain a homogeneous solution, add 1.2ml total 0.5mol / L rare earth chloride XCl mixed in proportion to this solution 3 (X=65mol%Lu, 30mol%Yb, 5mol%Dy) aqueous solution, stirred for 5min, then added 4ml NaF (1.0M) aqueous solution, stirred for 10min. The mixed solution was transferred to a 50ml hydrothermal kettle, and hydrothermally treated at 160°C for 16h. After natural cooling, add 20 ml of n-hexane to dissolve the precipitate, add 20 ml of ethanol to precipitate the precipitate and collect it by centrifugation, wash the precipitate with n-hexane 3 times (10 ml each time), and dry the precipitate in vacuum at -30°C for 10 hours to obtain KXF 4 (X = 65mol% Lu, 30mol% Yb, 5% mol Dy) mixed nanoparticles. identified, such as image 3 As shown in a, the particle size of the nanoparticle is abo...

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Abstract

The invention relates to an application of nanoparticles with fluoride lutecium as a main component in biological detection and preparation of a reagent for biological detection. Furthermore, the invention also relates to the nanoparticles used in the above application and a preparation method thereof. The dimension of the material is less than 200nm. The material has an excellent luminescence property and can be used in bioimaging and biodetection as a luminescent probe.

Description

technical field [0001] The invention belongs to the technical field of biological detection, in particular, the invention relates to the application of nano particles with lutetium fluoride as the main component in biological detection and preparation of reagents for biological detection. In addition, the present invention also relates to nanoparticles used in the above-mentioned applications and methods for their preparation. Background technique [0002] Nanoscale rare earth materials (also known as rare earth nanomaterials) exhibit unique optical, electrical, and magnetic properties due to their unique 4f electronic structure, especially rare earth up-conversion luminescent nanomaterials can absorb two or more low-energy A photon of high energy (ultraviolet or visible light) is emitted. Rare earth up-conversion luminescent nanomaterials are usually composed of host materials, luminescent rare earth ions and sensitized rare earth ions. Among them, the main rare earth ion...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
Inventor 李富友刘倩杨天赦
Owner FUDAN UNIV
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