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In-vitro regeneration culture method for downy grape

A technology of in vitro regeneration and cultivation method, which is applied in the field of agricultural science and achieves the effects of low cost, simple method and good use effect.

Inactive Publication Date: 2013-10-23
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The key to transgenic technology is the establishment of an efficient regeneration system, but there is no report on the regeneration of grapevine in vitro

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Embodiment 1 of the present invention: the in vitro regeneration and cultivation method of hairy grapes, comprising the following steps,

[0015] Step 1. Establishment of the sterile line: Select the vigorously growing shoots of the "Huaxi-4" grape as explants during the growing season, rinse them under tap water, and soak them in 70% alcohol by volume for 30 seconds. Rinse with sterile water for 3 times, then immerse and disinfect with 0.1% mercury liter solution for 8 minutes, rinse with sterile water for 5 times, cut into single-bud stem segments, and inoculate them on MS medium (add 2.0 mg of per liter of MS medium) 6-benzylpurine, 0.2mg indole acetic acid, 20g sucrose and 5g agar powder, pH5.8-6.0), the temperature of MS medium is 25±1℃, and the light intensity is 40umol·s -1 m -2 , the light time is 12h / d; cultivate and induce until the axillary buds germinate, and obtain the induced axillary bud leaves;

[0016] Step 2, induction of adventitious buds: take the ...

Embodiment 2

[0019] Embodiment 2 of the present invention: the in vitro regeneration and cultivation method of hairy grapes, comprising the following steps,

[0020] Step 1. Establishment of the sterile line: Select the vigorously growing shoots of the “Nongyuan-11” grape as explants during the growing season, rinse them under tap water, and then soak them in 70% alcohol by volume for 30 seconds , washed 3 times with sterile water, then soaked and sterilized with 0.1% mercury liter solution for 8 minutes, rinsed 5 times with sterile water, cut into single-bud stem segments, and inoculated on MS medium (adding 2.0 per liter of MS medium) mg of 6-benzylpurine, 0.2 mg of indole acetic acid, 20 g of sucrose and 5 g of agar powder, pH5.8-6.0), the temperature of MS medium is 25±1°C, and the light intensity is 40umol·s -1 m -2 , the light time is 12h / d; cultivate and induce until the axillary buds germinate, and obtain the induced axillary bud leaves;

[0021] Step 2, induction of adventitious...

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PUM

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Abstract

The invention discloses an in-vitro regeneration culture method for a downy grape. The in-vitro regeneration culture method comprises the following step: establishment of a sterile system, induction of adventitious buds, rooting culture and seedling exercising and transplanting. A plant tissue culture technology is adopted to successfully achieve in-vitro regeneration of downy grape leaf blades, the regeneration rate of the downy grape can be over 37% and can be 93.3% at the highest, the rooting rate can be over 90%, the survival rate can be 100%, a solid foundation is laid for genetic transformation of the downy grape, and an effective way is provided for transfer of other merits of grapes to the downy grape. The in-vitro regeneration culture method for the downy grape is simple, low in cost and good in using effect.

Description

technical field [0001] The invention relates to the field of agricultural science, in particular to a method for in vitro regeneration and cultivation of hairy grapes. Background technique [0002] Hairy grapes ( Vitis heyneana Roem. & Schult.), also known as downy grapes and pentagonal leaf grapes, are distributed in central China, east China, southwest, north south China, Shaanxi in northwest China, Gansu in northwest China, and Shanxi in north China. It is one of the most widely distributed wild grape species. Vitis vinifera has strong resistance, good adaptability, high fruiting coefficient, and is easy to hybridize with grape cultivars. It is not only an excellent resistant rootstock for viticulture, but also an excellent parent for grape resistance breeding. Due to the complex genetic background and long life cycle of grapes, conventional cross-breeding is greatly limited in variety improvement. Through transgenic technology, the resistance traits of grape grapes can ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 潘学军刘伟张文娥马孟增
Owner GUIZHOU UNIV
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