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Method for preparing high-purity cornflower pigment

A technology of cornflower pigment and high purity is applied in the field of preparation of high-purity cornflower pigment to achieve the effects of low cost, simple operation and low price

Inactive Publication Date: 2012-10-24
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Now the commercially available cyanidin monomer with 95% purity is RMB 842.4 / mg (http: / / www.sigmaaldrich.com / china-mainland.html), and there is no relevant literature report on the preparation of cyanidin monomer

Method used

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  • Method for preparing high-purity cornflower pigment

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Experimental program
Comparison scheme
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Embodiment

[0027] Main equipment: 1. High performance liquid chromatography (HPLC); 2. Constant temperature water bath; 3. Rotary evaporator; 4. Centrifuge; 5. Crushing machine; 6. AB-8 macroporous resin column; 7. Nitrogen Blow instrument.

[0028] Materials and Reagents:

[0029] 1. Tested material: tea tree root (stored at -80°C for later use);

[0030] 2. Main reagents:

[0031] Acetone (analytical pure), acetic acid (analytical pure), methanol (analytical pure), n-butanol (analytical pure), ethyl acetate (analytical pure), concentrated hydrochloric acid (analytical pure).

[0032] Extract solution: Take 70 ml of acetone, add 29.5 ml of water and 0.5 ml of acetic acid and shake well.

[0033] Pyrolysis solution: Take 95 ml of n-butanol and add 5 ml of concentrated hydrochloric acid.

[0034] 20% methanol: add 20ml methanol to 80ml water and shake well.

[0035] The specific operation steps for preparing high-purity cyanidin are as follows:

[0036] 1) Extraction of proanthocyan...

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Abstract

The invention relates to a method for preparing high-purity cornflower pigment. The method comprises the operation steps as follows: 1, extracting procyanidins, and preparing procyanidins solution using fresh tea tree root; 2, purifying the procyanidins solution, and preparing the procyanidins crude solution; 3, preparing the cornflower pigment methanol solution from the procyanidins crude solution; 4, purifying the cornflower pigment to obtain the cornflower pigment solution; and 5, carrying out rotary evaporation and other treatments on the cornflower pigment solution to obtain the cornflower pigment powder with the purity of above 98%, wherein the cornflower pigment powder is carmine, is difficult to dissolve in water, and is freely soluble in methanol. The method is simple to operate, no large expensive experimental apparatus is needed, and the adopted organic solvent is low in price and can be recovered, so the cost is low; and the method can effectively remove sugar, protein, other types of anthocyanidins and flavonoids compounds of the extract, and is suitable for obtaining the high-purity cornflower product.

Description

technical field [0001] The invention belongs to the field of natural product chemistry and relates to a preparation method of high-purity cyanidin. Background technique [0002] The cyanidin is one of the anthocyanin compounds. The free cyanidin monomer is very rare under natural conditions. It mainly exists in the form of glycosides, often combined with one or more glucose (glucose), rhamna Sugar (rhamnose), galactose (galactose), xylose (xylose), arabinose (arabinose) and so on are connected by glycosidic bonds to form anthocyanin glycosides. Glycosidized cyanidins are widely found in the cell fluid of flowers, fruits, stems, leaves and root organs of flowering plants (angiosperms), distributed in 27 families and 72 genera of plants, especially grape skin , mulberry, blackberry, blueberry, cherry, hawthorn, raspberry, purple cabbage, etc. are the most abundant. [0003] Like other types of anthocyanins, cyanidin is not only a safe natural edible red pigment, but also has...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/62C09B61/00
Inventor 夏涛蒋晓岚刘亚军杨琴高丽萍
Owner ANHUI AGRICULTURAL UNIVERSITY
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