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Production process of Jew's ear melanin by fermentation and products of Jew's ear melanin

A production process and technology of black fungus, applied in the production process of black fungus melanin and its products, can solve the problems of complex process, high production cost, poor product safety, etc., and achieve simple production process, low cost and short production cycle Effect

Inactive Publication Date: 2014-05-21
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Technical problem: The present invention provides a production process and its products for preparing black fungus melanin by fermentation method, so as to solve technical problems such as high production cost, complicated process and poor product safety in the current melanin production process

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Take black fungus preserved strains and inoculate them on the inclined surface of test tubes equipped with activation medium for activation and culture for 7 days, pick the activated hyphae and insert them into solid plate medium (composed of: 20% potato, 2% glucose, 2% agar) , cultured in the dark at 25°C for 6 days, took four 6mm-diameter bacterial blocks at the edge of the colony with a puncher and put them into the seed medium (composed of: 20% wheat bran juice, 0.1% magnesium sulfate, 0.1% potassium dihydrogen phosphate, vitamin B1 0.01%), cultured at 25°C in the dark for 3 days. The liquid submerged fermentation medium (composed of: 35% of wheat bran juice, 1.1g / L of casein, 0.15g / L of copper sulfate, 0.1% of magnesium sulfate) by inoculating the seed liquid into the initial pH of 8.0 by the inoculation amount of 8% , potassium dihydrogen phosphate 0.1%, vitamin B 1 0.01%), 25°C, 100r / min in the dark and shake culture for 6 days. Add tyrosine at a concentration...

Embodiment 2

[0024] The activation of the black fungus strain and the preparation of the seed solution are the same as in Example 1, and the seed solution is inoculated to the liquid submerged fermentation medium (composed of: 30% wheat bran juice, 1.5g casein) with an initial pH of 7.0 by an inoculum size of 6%. / L, copper sulfate 0.1g / L, magnesium sulfate 0.1%, potassium dihydrogen phosphate 0.1%, vitamin B 1 0.01%), culture at 25°C, 100r / min in the dark and shake for 6 days. Tyrosine was added at a concentration of 1.0 g / L on the 2nd day of fermentation, and tyrosine was added at a concentration of 1.0 g / L on the 4th day. After the fermentation, the fermented liquid treatment, melanin purification and drying process were the same as in Example 1 to obtain black fungus melanin powder.

Embodiment 3

[0026] The activation of black fungus strains and the preparation of seed solution are the same as in Example 1, and the seed solution is inoculated to the fermentation medium (composed of: wheat bran juice 35%, casein 1.1g / L) to an initial pH of 8.0 by an inoculum size of 5%. , copper sulfate 0.15g / L, magnesium sulfate 0.1%, potassium dihydrogen phosphate 0.1%, vitamin B 1 0.01%), culture at 25°C, 100r / min in the dark and shake for 6 days. Tyrosine was added at a concentration of 1.5 g / L on the 2nd day of fermentation, and tyrosine was added at a concentration of 1.0 g / L on the 4th day. After the fermentation, the fermented liquid treatment, melanin purification and drying process were the same as in Example 1 to obtain black fungus black fungus melanin powder.

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PUM

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Abstract

The invention relates to a production process of Jew's ear melanin by fermentation and products of the Jew's ear melanin. The production process is characterized by using wheat bran juice as main component, adding casein, copper sulfate, monopotassium phosphate and the like to form fermentation medium, inoculating Auricularia auricular mycelium, and performing liquid subsurface fermentation, separation and purification, and freeze-drying to obtain the Jew's ear melanin high in purity and safe to eat. The production process has the advantages of short production cycle, low cost, high yield, convenience for subsequent processing and the like, the Jew's ear melanin is powdered and has purity up to 70-85% and yield of 900-1100mg / L.

Description

1. Technical field [0001] The invention relates to a production process of black fungus melanin and a product thereof, which belongs to the field of food biotechnology and also belongs to the technical field of natural food pigment production. 2. Background technology [0002] Melanin is a polymer formed by oxidative polymerization of polyphenols or indole compounds, which has physiological functions such as anti-oxidation, anti-radiation, anti-HIV and immune regulation. Melanin derived from animals, plants or microorganisms has safety and biological activity, especially as a substitute for synthetic pigments, and has huge market demand and development potential. [0003] At present, the production methods of melanin mainly include chemical extraction, oxidation synthesis and biological fermentation. Some preparation methods of melanin are disclosed domestically, and patent (publication number CN 101012340A, published on August 8, 2007) discloses "a kind of method for extra...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P1/02
Inventor 顾振新邹宇尹永祺
Owner NANJING AGRICULTURAL UNIVERSITY
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