Production process of Jew's ear melanin by fermentation and products of Jew's ear melanin
A production process and technology of black fungus, applied in the production process of black fungus melanin and its products, can solve the problems of complex process, high production cost, poor product safety, etc., and achieve simple production process, low cost and short production cycle Effect
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Embodiment 1
[0022] Take black fungus preserved strains and inoculate them on the inclined surface of test tubes equipped with activation medium for activation and culture for 7 days, pick the activated hyphae and insert them into solid plate medium (composed of: 20% potato, 2% glucose, 2% agar) , cultured in the dark at 25°C for 6 days, took four 6mm-diameter bacterial blocks at the edge of the colony with a puncher and put them into the seed medium (composed of: 20% wheat bran juice, 0.1% magnesium sulfate, 0.1% potassium dihydrogen phosphate, vitamin B1 0.01%), cultured at 25°C in the dark for 3 days. The liquid submerged fermentation medium (composed of: 35% of wheat bran juice, 1.1g / L of casein, 0.15g / L of copper sulfate, 0.1% of magnesium sulfate) by inoculating the seed liquid into the initial pH of 8.0 by the inoculation amount of 8% , potassium dihydrogen phosphate 0.1%, vitamin B 1 0.01%), 25°C, 100r / min in the dark and shake culture for 6 days. Add tyrosine at a concentration...
Embodiment 2
[0024] The activation of the black fungus strain and the preparation of the seed solution are the same as in Example 1, and the seed solution is inoculated to the liquid submerged fermentation medium (composed of: 30% wheat bran juice, 1.5g casein) with an initial pH of 7.0 by an inoculum size of 6%. / L, copper sulfate 0.1g / L, magnesium sulfate 0.1%, potassium dihydrogen phosphate 0.1%, vitamin B 1 0.01%), culture at 25°C, 100r / min in the dark and shake for 6 days. Tyrosine was added at a concentration of 1.0 g / L on the 2nd day of fermentation, and tyrosine was added at a concentration of 1.0 g / L on the 4th day. After the fermentation, the fermented liquid treatment, melanin purification and drying process were the same as in Example 1 to obtain black fungus melanin powder.
Embodiment 3
[0026] The activation of black fungus strains and the preparation of seed solution are the same as in Example 1, and the seed solution is inoculated to the fermentation medium (composed of: wheat bran juice 35%, casein 1.1g / L) to an initial pH of 8.0 by an inoculum size of 5%. , copper sulfate 0.15g / L, magnesium sulfate 0.1%, potassium dihydrogen phosphate 0.1%, vitamin B 1 0.01%), culture at 25°C, 100r / min in the dark and shake for 6 days. Tyrosine was added at a concentration of 1.5 g / L on the 2nd day of fermentation, and tyrosine was added at a concentration of 1.0 g / L on the 4th day. After the fermentation, the fermented liquid treatment, melanin purification and drying process were the same as in Example 1 to obtain black fungus black fungus melanin powder.
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