Chinese isolated strain of bovine enterovirus, and construction and application of infectious cDNA clone thereof

A technology of enteroviruses and isolates, applied in the field of bovine enteroviruses, to achieve the effect of easy preservation
CN102776155AActive Publication Date: 2012-11-14HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
Publication Date
2012-11-14

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Abstract

The invention relates to a Chinese isolated strain of bovine enterovirus, and constructions and applications of infectious cDNA clone thereof. Isolated strain of bovine enterovirus capable of stably generating CPE on MA104 cells can be separated from bovine diarreal stool samples, with a microbial preservation number of CGMCC No. 4782. Whole-length infectious cDNA clone is constructed from the isolated strain. B family gene type 2 bovine enterovirus rescue virus is obtained by in-vitro transcribing the cDNA and transfectioning cells. The isolated strain of bovine enterovirus, the constructed whole-length infectious cDNA clone and the rescue virus thereof can be used for researches of gene structures and functions of bovine enterovirus, can be used as vaccine vectors of bovine enterovirus, or can be prepared into vaccines or reagents capable of preventing or treating bovine enterovirus.
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Description

technical field

[0001] The present invention relates to bovine enterovirus strains and infectious cDNA clones thereof, in particular to Chinese isolates of bovine enteroviruses. The present invention also relates to the construction and application of infectious cDNA clones of the Chinese isolates of bovine enteroviruses, belonging to bovine enteroviruses. Enterovirus field. Background technique

[0002] Bovine Enterovirus (BEV) is a member of the genus Enterovirus in the Picornaviridae family. Virus particles are spherical, icosahedral, without envelope, and the diameter of the complete virus particle is about 25-30nm. The genome is a single-stranded positive-strand RNA, consisting of about 7500 nucleotides (nt), which can be directly used as mRNA to encode and translate a polyprotein, and then undergo a series of degradation to produce four structural proteins (VP1, VP2, VP3 and VP4) and seven non-junction proteins (2A, 2B, 2C, 3A, 3B, 3C and 3D). The capsid of BEV is i...

Claims

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