Magnetic microzyme surface imprinted adsorbent for selectively separating cefalexin as well as preparation method and application thereof

A technology of cephalexin and surface imprinting, applied in chemical instruments and methods, other chemical processes, alkali metal oxides/hydroxides, etc., to achieve the effects of less by-products, high activity, and easy access

Inactive Publication Date: 2012-11-21
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the magnetic composite material is prepared by immobilizing the metal oxide on the yeast or coating the yeast with a polymer material, and then using the a

Method used

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  • Magnetic microzyme surface imprinted adsorbent for selectively separating cefalexin as well as preparation method and application thereof
  • Magnetic microzyme surface imprinted adsorbent for selectively separating cefalexin as well as preparation method and application thereof
  • Magnetic microzyme surface imprinted adsorbent for selectively separating cefalexin as well as preparation method and application thereof

Examples

Experimental program
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Embodiment 1

[0035] (1) Preparation of magnetic yeast composite material

[0036] Step A. Saccharomycetes is dispersed in saturated sodium chloride aqueous solution at normal temperature, the mass and volume ratio of yeast and saturated sodium chloride aqueous solution is 1g:5mL, forms yeast bacterium solution after mechanical stirring 30min;

[0037] Step B. take chitosan by weighing the ratio of 1g:35mL and be dispersed in 0.1mol / L acetic acid solution, mechanically stir 0.5h, form chitosan colloidal liquid;

[0038] Step C. The yeast solution is added to the chitosan colloidal liquid, the mass ratio of yeast to chitosan is 1:0.5 (g), and then adding the yeast mass ratio is 0.1: 1 (g) gamma-Fe 2 o 3 , after stirring for 1.5h, add paraffin oil and Span-80 with a volume ratio of 20:0.5 (mL), wherein the volume and mass ratio of paraffin oil to yeast is 100mL:1g, after emulsification for 20min, add dropwise with paraffin oil 25% (v / v) glutaraldehyde cross-linking agent with a volume ratio ...

Embodiment 2

[0051] (1) Preparation of magnetic yeast composite material

[0052] Step A. Disperse yeast in saturated aqueous sodium chloride solution at normal temperature, the mass and volume ratio of yeast to saturated aqueous sodium chloride solution is 1g:25mL, and form yeast solution after mechanical stirring for 30min;

[0053] Step B. take chitosan by weighing the ratio of 1g:60mL and be dispersed in 0.1mol / L acetic acid, mechanically stir for 0.5h, form chitosan colloidal liquid;

[0054] Step C. Add the yeast solution into the jelly liquid of chitosan, the mass ratio of yeast to chitosan is 1:2 (g), and then add the yeast with a mass ratio of 0.5 :1(g) of γ-Fe 2 o 3 , after stirring for 1.5h, add paraffin oil and Span-80 with a volume ratio of 20:2 (mL), wherein the volume and mass ratio of paraffin oil to yeast is 180mL:1g, after emulsification for 40min, add dropwise with paraffin oil 25% (v / v) glutaraldehyde cross-linking agent with a volume ratio of 1.5:25 (mL), cross-link...

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Abstract

The invention relates to a magnetic microzyme surface imprinted adsorbent for selectively separating cefalexin as well as a preparation method and application thereof, belonging to the technical fields of material preparation and environment pollution improvement. The preparation method comprises the following steps of: firstly, coating nanometer gamma-Fe2O3 particles on the surface of microzyme to form a magnetic microzyme composite material by using cross-linking chitosan; secondly, carrying out surface modification on the magnetic microzyme composite material to form a magnetic microzyme composite material with an initiator; and finally, with the cefalexin as a template molecule, methylacrylic acid as a function monomer, ethylene glycol-bis(methylacrylic acid) ester as a cross-linking monomer, cuprous chloride as a catalyst, carrying out atom transfer radical polymerization to finish the preparation. The obtained adsorbent has an adsorptivity, selective recognition and enriching performance for the cefalexin and superparamagnetism for an external magnetic field and can be applied to selective recognition and separation of the cefalexin in an aqueous solution.

Description

technical field [0001] The invention belongs to the technical field of material preparation and environmental pollution control, relates to the field of magnetic yeast surface imprinted polymers and preparation methods thereof, in particular to a magnetic yeast surface imprinted adsorbent capable of selectively separating cephalexin and its preparation method and application. Background technique [0002] Cephalosporins, including cephalexin, are β-lactam antibiotics that are widely used to treat respiratory tract infections, prostatitis, urinary tract infections, and skin and soft tissue infections. β-Lactam antimicrobials are among the most widely used antimicrobials since 70 years ago, and environmental exposure to widely used antibiotics has raised concerns. Prolonged exposure to β-lactam antimicrobials has been shown to cause allergic and toxic reactions in sensitive individuals and facilitate bacterial transmission, which poses a potential threat to public health. Th...

Claims

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Application Information

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IPC IPC(8): B01J20/26B01J20/28B01J20/30C08F292/00C08F293/00C08F222/14C08F220/06C08J9/26C07D501/22C07D501/12
Inventor 潘建明李秀秀戴江栋卫潇闫永胜
Owner JIANGSU UNIV
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