Simple identification method for leather fiber structure

Abstract

The invention relates to the technical field of identification for leather materials, in particular to a simple identification method for a leather fiber structure. The simple identification method includes: firstly, flattening an original leather sample and preconditioning for 48 hours in atmospheric conditions; secondly, fabricating the leather sample subjected to preconditioning to a section test sample, and standing in the atmospheric conditions; thirdly, placing the stood section test sample into a super-depth-of-field three-dimensional video microscope for visualization, and shooting microscopic images; and fourthly, identifying the leather fiber structure according to leather section microscopic characteristics displayed by the microscopic images. The simple identification method for the leather fiber structure has the advantages that complicated procedures of ultrathin section, dyeing and the like in a traditional tissue section process can be omitted, and the simple identification method is simple in sample preparation, small in leather fiber deformation, clear in edge contours and internal characteristics and capable of accurately reflecting the leather fiber structure, and the like.

Description

technical field [0001] The invention relates to the technical field of identification of leather materials, in particular to a simple identification method for leather fiber structure. Background technique [0002] Leather is a kind of biological tissue. To understand the structure and shape of various raw leather and its changes in the production process, it must be studied through histological methods. At present, it is mainly through scanning electron microscopy (SEM), transmission electron microscopy (TEM) ), atomic force microscopy (AFM) and optical microscopy (LM) to study the fiber morphology. [0003] The use of tissue section observation to study the fiber changes of animal leather before and after processing has been very mature. Since the 1950s, it has been used to study pigskin, sheepskin, rabbit skin, deerskin, snakeskin and other leather and fur in China. , the observation results are used to guide the processing and production and the research and development...

AI Technical Summary

Problems solved by technology

For the report of tissue section observation leather structure: Li Jianmin et al. observed the existence and distribution of chromium ions in collagen fibers with transmission electron microscope observation and electron probe X-ray micro-area analysis, and explored its impact on the quality and quality of finished leather. performance and other aspects (a method for ultramicroscopic positioning of chromium ions in leather collagen fibers, 1985); Li Wei observed the tissue sections of leather with a microscope, and studied the influence of the loose surface on the mechanical properties of leather (observed the loose surface of leather with a microscope and its influence on the mechanical properties of leather, 2010); Wang Yingmei from Sichuan University and others studied the tissue structure of suckling pig skin by observing slices of suckling pig skin through a polarizing microscope (study on the tissue structure of suckling pig skin, 2002); Lu from Shaanxi University of Science and Technology The tissue sections of finished pig, sheep and cowhide leathers with different hand textures were prepared by static nierland dyeing with sulfuric acid, and their structure was observed under a multimedia microscope (discussion on the relationship between the structure and properties of finished pig, sheep and cowhide leather, 2010) ; Patents such as Zhang Huayong introduced a sample preparation method suitable for metallographic microscope observation of leather tissue, and its production process is relatively complicated (a leather sample preparation method suitable for metallographic microscope observation, application number: 201110179680.4)

[0004] However, the general thickness of slices in slicing processing is 5-25 microns, and the ultra-thin slice thickness of transmission electron microscope is 500-700 angstroms, which requires advanced sample preparation instruments and skilled sample preparation skills, and the sample tissue is very difficult for the sliced The pressure of the knife, the embedding agent will compress and shrink during the embedding process, which will affect the observation quality of the optical microscopic image

In addition, the fibers in the leather are dense, criss-cross, and uneven in thickness. During the slicing process, fibers are prone to extrusion deformation, shedding, or inter-fiber adhesion, resulting in deformation of the fiber cross-section of the sliced ​​leather sample, and it is difficult to extract accurate cross-sectional characteristic parameters.

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