Inactivated vaccine preparation method for egg yolk antibody for resisting litopenaeus vannamei red body disease

An inactivated vaccine, the technology of Vannabine, which is applied in the field of inactivated vaccine preparation method of egg yolk antibody, can solve the problems such as shortage, and achieve the effects of increasing production, improving survival rate, and enhancing immunity

Inactive Publication Date: 2012-12-26
HUAIHAI INST OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, it is difficult to control the occurrence and prevalence of diseases caused by Vibrio parahaemolyticus in mariculture production of Litopenaeus vannamei, and there is no convenient inactivated vaccine against red body disease of Litopenaeus vannamei in the market

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  • Inactivated vaccine preparation method for egg yolk antibody for resisting litopenaeus vannamei red body disease

Examples

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Embodiment 1

[0017] Example 1, a preparation method of an inactivated vaccine for preparing yolk antibody against Litopenaeus vannamei red body disease, which uses Vibrio parahaemolyticus JGB080708, the pathogen of Litopenaeus vannamei red body disease inactivated by formaldehyde solution -1( Vibrio parahaemolyticus ) as an immunogen, using white oil, Span 80, aluminum stearate as an oil emulsion, and astragalus extract as an immune enhancer, emulsified to prepare an oil emulsion inactivated vaccine; the specific steps are as follows:

[0018] (1) Inoculate the pathogenic Vibrio parahaemolyticus JGB080708-1 strain on ordinary nutrient agar broth medium, culture on a shaker at 30°C for 24h, add formaldehyde with a final volume concentration of 0.3% at 28°C for 48h, and centrifuge to obtain inactivated bacteria body;

[0019] (2) Soak Astragalus membranaceus in distilled water for 60 minutes, decoct, filter, concentrate to a final concentration of 1 g / mL of the original drug, and obtain As...

Embodiment 2

[0023] Example 2, an inactivated vaccine preparation method for preparing yolk antibody against Litopenaeus vannamei red body disease, which uses Vibrio parahaemolyticus JGB080708, the pathogen of Litopenaeus vannamei red body disease inactivated by formaldehyde solution -1( Vibrio parahaemolyticus ) as an immunogen, using white oil, Span 80, aluminum stearate as an oil emulsion, and astragalus extract as an immune enhancer, emulsified to prepare an oil emulsion inactivated vaccine; the specific steps are as follows:

[0024] (1) Inoculate the pathogenic Vibrio parahaemolyticus JGB080708-1 strain on ordinary nutrient agar broth medium, culture on a shaker at 29°C for 24 hours, add formaldehyde with a final volume concentration of 0.3% at 28°C for 48 hours, and centrifuge to obtain inactivated bacteria body;

[0025] (2) Take Astragalus membranaceus soaked in distilled water for 30 minutes, decoct, filter, and concentrate until the final concentration is 1g / mL of the original...

Embodiment 3

[0029] Example 3, an inactivated vaccine preparation method for preparing yolk antibody against Litopenaeus vannamei red body disease, which uses Vibrio parahaemolyticus JGB080708, the pathogen of Litopenaeus vannamei red body disease inactivated by formaldehyde solution -1( Vibrio parahaemolyticus ) as an immunogen, using white oil, Span 80, aluminum stearate as an oil emulsion, and astragalus extract as an immune enhancer, emulsified to prepare an oil emulsion inactivated vaccine; the specific steps are as follows:

[0030] (1) Inoculate the pathogenic Vibrio parahaemolyticus JGB080708-1 strain on ordinary nutrient agar broth medium, culture on a shaker at 28°C for 24h, add formaldehyde with a final volume concentration of 0.3% at 28°C for 48h, and centrifuge to obtain inactivated bacteria body;

[0031] (2) Soak Astragalus membranaceus in distilled water for 45 minutes, decoct, filter, concentrate to a final concentration of 1 g / mL of the original drug, and obtain Astraga...

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Abstract

The invention provides an inactivated vaccine preparation method for an egg yolk antibody for resisting a litopenaeus vannamei red body disease. The inactivated vaccine preparation method comprises the steps of inactivating etiology vibrio parahaemolyticus JGB080708-1 bacterial strains and centrifuging to obtain inactivation thalli; preparing astragalus mongholicus lixiviums; preparing an oil phase by using white oil, aluminium stearate and span-80 and mixing the inactivation thalli and the astragalus mongholicus lixiviums to prepare a vaccine aqueous phase; and mixing the oil phase and the aqueous phase according to a volume ratio of 2:1 for emulsification and grinding for 5min to prepare vibrio parahaemolyticus oil emu inactivated vaccines with the astragalus mongholicus and the white oil serving as a unite adjuvant. The vibrio parahaemolyticus oil emulsion inactivated vaccines can be used for immunization of adult laying hens, the high egg yolk antibody for resisting the litopenaeus vannamei red body disease is further obtained, the immunity of litopenaeus vannamei etiology vibrio parahaemolyticus is strengthened, the rate of survival of litopenaeus vannamei culture is improved, the yield of the litopenaeus vannamei culture is increased, and economic benefits of litopenaeus vannamei culture and production are improved. A problem of incidental vibrio parahaemolyticus caused diseases in the litopenaeus vannamei culture and production process at present is fundamentally solved.

Description

technical field [0001] The invention relates to a preparation method of an inactivated vaccine, in particular to an inactivated vaccine preparation method for preparing yolk antibody against the red body disease of Litopenaeus vannamei. Background technique [0002] Litopenaeus vannamei ( Litopenaeus vannamei ), also known as white prawn, Penaeus wanshii, etc., is a tropical shrimp species, which has the advantages of wide salt range, tolerance to close culture, fast growth, and strong stress resistance. After being introduced in my country in 1988, it is now in coastal areas of the country Large-scale farming in various places. With the expansion of the culture scale of Litopenaeus vannamei, the ecological environment of the culture is deteriorating day by day, and the occurrence frequency of diseases rises sharply. The pathogen Vibrio parahaemolyticus ( Vibrio parahaemolyticus ) caused by red body disease is widely prevalent in all shrimp farming areas across the country...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/39A61K39/106A61K9/107A61P31/04A61K39/40
Inventor 秦国民张晓君闫斌伦毕可然陈丽
Owner HUAIHAI INST OF TECH
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