Method of extracting gutta-percha through enzymolysis

A technology of eucommia gum and enzymolysis is applied in the field of enzymatic hydrolysis and extraction of eucommia gum, which can solve the problems of long regeneration cycle of callus, low purity of gum silk, low extraction yield, etc., so as to improve solvent utilization efficiency and save process operation. time, the effect of improving filtration efficiency

Inactive Publication Date: 2015-04-29
HENAN HENGRUIYUAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantages of this method are: serious damage to the glue filament, poor physical properties, high toxicity of the solvent, unrecyclable, complicated process, serious pollution, and high cost
The disadvantages of this method are: the cooking temperature is high, the rubber filament is destroyed, the pollution is large, the purity of the rubber filament is not high, and the production cycle is long
The disadvantages of this method are: the mixed solvent cannot be used, the cost is high, the regeneration cycle of the wounded tissue is long, and the leather toxicity is large.
The disadvantages of this method are: serious pollution by concentrated acid and concentrated alkali, large amount of sewage discharge, serious damage to Eucommia gum by alkali, decline in physical properties, and the product is coarse rubber
[0013] According to the analysis of the above status quo, no matter which of the above extraction methods, there are many defects in the process, such as many times of extraction, heavy environmental pollution, and low extraction yield.
Therefore, the current bottleneck in realizing the industrialization of Eucommia rubber extraction is mainly the problem of high processing and extraction costs and poor extraction accuracy.

Method used

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  • Method of extracting gutta-percha through enzymolysis
  • Method of extracting gutta-percha through enzymolysis
  • Method of extracting gutta-percha through enzymolysis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] A preparation method of eucommia gum, which is made from natural eucommia ulmoides bark, eucommia leaves or eucommia samara skin, the molecular weight of eucommia ulmoides gum is 200,000-300,000, and the impurity content in eucommia gum is less than 0.5%. The specific preparation steps are as follows:

[0042] Step 1. Select Eucommia bark, wash it in clear water, and dry it so that the water content of Eucommia bark is less than 5%, and set aside;

[0043] Step 2: Pretreatment by enzymatic hydrolysis

[0044] A, prepare enzyme solution: take 0.27% cellulase, 0.01% pectinase and 0.02% protease to dissolve in water respectively by mass percentage, be mixed with the composite enzyme liquid containing cellulase, pectinase and protease;

[0045] B. Add the Eucommia bark cleaned in the above step 1 to the prepared compound enzyme solution containing cellulase, pectinase and protease at a material-to-liquid ratio of 1:1; stir at 50°C and 500r / min Under enzymatic hydrolysis fo...

Embodiment 2

[0051] A preparation method of eucommia gum, which is made from natural eucommia ulmoides bark, eucommia leaves or eucommia samara skin, the molecular weight of eucommia ulmoides gum is 200,000-300,000, and the impurity content in eucommia gum is less than 0.5%. The specific preparation steps are as follows:

[0052] Step 1. Select leaves of eucommia ulmoides, wash them in clear water, and dry them so that the water content in eucommia bark, eucommia leaves or eucommia seeds is less than 5%, and set aside;

[0053] Step 2: Pretreatment by enzymatic hydrolysis

[0054] A, prepare enzyme solution: take 0.27% cellulase, 0.01% pectinase and 0.02% protease to dissolve in water respectively by mass percentage, be mixed with the composite enzyme liquid containing cellulase, pectinase and protease;

[0055] B. Add the Eucommia ulmoides leaves cleaned in the above step 1 to the prepared compound enzyme liquid containing cellulase, pectinase and protease according to the ratio of materi...

Embodiment 3

[0061] A preparation method of eucommia gum, which is made from natural eucommia ulmoides bark, eucommia leaves or eucommia samara skin, the molecular weight of eucommia ulmoides gum is 200,000-300,000, and the impurity content in eucommia gum is less than 0.5%. The specific preparation steps are as follows:

[0062] Step 1, choose one of the eucommia samara skins, put into clear water, wash, and dry, so that the water content in the eucommia samara skins is less than 5%, and set aside;

[0063] Step 2: Pretreatment by enzymatic hydrolysis

[0064] A, prepare enzyme solution: take 0.27% cellulase, 0.01% pectinase and 0.02% protease to dissolve in water respectively by mass percentage, be mixed with the composite enzyme liquid containing cellulase, pectinase and protease;

[0065] B. Add the Eucommia ulmoides skin cleaned in the above step 1 into the prepared compound enzyme liquid containing cellulase, pectinase and protease at a material-to-liquid ratio of 1: 2; Enzymolysis ...

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Abstract

Provided is a method of extracting gutta-percha through enzymolysis. One of natural eucommia skin or eucommia leaves or eucommia wing skin is placed in clean water to be cleaned and dried, and moisture content of the eucommia skin, the eucommia leaves, or the eucommia wing skin is enabled to be less than 5%. A compound enzyme which comprises, by mass, 0.27% of cellulose, 0.01% of pectinase, and 0.02% of protease is added, enzymolysis undergoes 1-3 days under stirring at a speed of 500 r / min under 50 DEG C. Mixture after the enzymolysis is filtered, dregs of the filtered mixture are sent to an ultrasonic extraction tank for ultrasound extraction, and glue mixed liquor is produced. The glue mixed liquor is filtered in a grading mode, impurities are eliminated, and the glue mixed liquor with the impurities eliminated is sent to a separation tank. Eventually the gutta-percha is extracted through the method that gutta-percha crystals are precipitated at a low temperature. The method is simple in process, little in pollution, high in yield rate, low in cost, and ideal in effect; the extraction rate reaches more than 95%, and the gutta-percha content reaches 99.9%; and the produced gutta-percha is long in gelatin silk, high in tensile and tearing-proofing physical strength, and capable of being applied to wide fields.

Description

technical field [0001] The invention relates to a method for extracting natural products, in particular to a method for enzymatically extracting eucommia gum. Background technique [0002] Eucommia rubber is the only one in the world other than clover rubber (natural rubber) with huge [0003] development prospects of high-quality natural rubber resources, and the planting area of ​​Eucommia in my country accounts for the world's [0004] More than 99% of the planting area has a monopoly resource advantage. Some developed countries abroad, such as Japan, buy a lot of eucommia leaves from my country at high prices to speed up the research and development of new materials for eucommia gum. Therefore, the application and development of eucommia rubber are the key topics of national research. [0005] However, at present, the domestic industry does not have a high awareness of the development prospects of the eucommia industry, and has not formed an industrial chain for ups...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J3/00C08L7/00
Inventor 夏琳辛振祥魏军生李银环
Owner HENAN HENGRUIYUAN IND
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