Fermentation medium and fermentation method of aflatoxin B1

A fermentation medium and aflatoxin technology, applied in the field of fermentation engineering, can solve the problems of low spore vigor, low toxin production, long growth (recovery) cycle, etc., to achieve high spore vigor, easy access to raw materials, and short culture cycle Effect

Inactive Publication Date: 2013-01-02
ZHEJIANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The invention provides a fermentation medium for producing aflatoxin B1, which is used to solve the problems of low spore vigor, long growth (recovery) cycle, and low toxin production in the current traditional medium

Method used

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  • Fermentation medium and fermentation method of aflatoxin B1
  • Fermentation medium and fermentation method of aflatoxin B1
  • Fermentation medium and fermentation method of aflatoxin B1

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-4

[0021] The preparation of embodiment 1-4 Aspergillus flavus growth-promoting nutrient solution

[0022] After mixing the components shown in Table 1, adjust the pH value of the mixed solution to 5.5-7.0 with hydrochloric acid solution.

[0023] Table 1

[0024]

[0025] Now take Example 2 as an example to prepare 1 L of growth-promoting nutrient solution. Weigh 150g sucrose, 20g yeast extract, 20ml bran extract, 2gNaNO 3 , 0.3gMgSO 4 , 0.3gKCl, 0.8gK 2 HPO 4 , 0.01gFeSO 4 .7H 2 O, 810ml of distilled water was placed in a 2000ml Erlenmeyer flask, after heating to dissolve all the raw materials, use 5mol / L hydrochloric acid to adjust the pH value to 5.5-7.0. After pH adjustment, cover with a cotton plug, and sterilize at 121-123°C, 0.12MPa, for 20min. After cooling in the biosafety cabinet, the growth-promoting nutrient solution was prepared.

[0026] The preparation method of the bran extract is as follows (the same below): take 1 unit weight of bran and place it i...

Embodiment 5-8

[0027] Embodiment 5-8 Preparation of aspergillus flavus recovery medium

[0028] A recovery medium for Aspergillus flavus. After mixing the components shown in Table 2, the pH value of the mixed solution is adjusted to 5.5-7.0 with hydrochloric acid solution.

[0029] Table 2

[0030]

[0031] Now take the Aspergillus flavus recovery medium formula in Example 6 as an example to prepare 1L recovery medium. Weigh 150g sucrose, 20g yeast extract, 20ml bran extract, 2gNaNO 3 , 0.3gMgSO 4 , 0.3gKCl, 0.8gK 2 HPO 4 , 0.01gFeSO 4 .7H 2O, 15g of agar powder, 795ml of distilled water, placed in a 2000ml Erlenmeyer flask, after heating to dissolve all the raw materials, use 5mol / L hydrochloric acid to adjust the pH value to 5.5-7.0. After pH adjustment, cover with a cotton plug, and sterilize at 121-123°C, 0.12MPa, for 20min. In a biological safety cabinet, the sterilized resuscitation medium was not solidified at 60°C, and placed upside down in a sterilized 10 cm diameter p...

Embodiment 9-12

[0034] The preparation of embodiment 9-12 Aspergillus flavus solid fermentation medium

[0035] According to the recipe in Table 3, prepare the required medium.

[0036] table 3

[0037]

[0038] Raw material preparation: crush indica rice, soybean meal and bran, the particle size is required to pass through a 20-mesh standard sieve, but not through a 40-mesh standard sieve, that is, between 20 and 40 mesh.

[0039] Now take the components of Example 10 as an example to prepare 1 kg of solid fermentation medium. Weigh 120g of sucrose, 20g of yeast extract, 30g of bran, 750g of indica rice, 80g of soybean meal, and another 80ml of distilled water, mix the above materials evenly, put them in a 3000ml Erlenmeyer flask, cover with a cotton plug, and store at 121-123°C , 0.12MPa, sterilization for 20min. In a biological safety cabinet, pour the sterilized solid medium upside down in a sterilized Erlenmeyer flask with a thickness of 1cm, or a load of 50g / 500ml Erlenmeyer fla...

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Abstract

The invention belongs to the technical field of fermentation engineering, and especially relates to a fermentation medium and a fermentation method for producing aflatoxin B1. The fermentation medium of aflatoxin B1 contains a yeast extract which accounts for 1-5% of the total weight of the medium; the fermentation medium comprises a recovery medium, a solid fermentation medium or a growth promotion nutrient solution. The fermentation method of aflatoxin B1 comprises two steps of strain recovery and solid fermentation culture; aspergillus flavus is employed for fermentation; the fermentation medium provided by the invention is employed for strain recovery and solid fermentation culture. According to the medium and culture procedure provided by the invention, the concentration in a solid air-dried substance of the fermentation medium of aflatoxin B1 is up to 7349 ppb; when the fermentation medium and the fermentation method of the invention are compared with the prior art with respect to fermentation titer, significant advancement is provided.

Description

technical field [0001] The invention belongs to the technical field of fermentation engineering, in particular to a fermentation medium and a fermentation method for producing aflatoxin B1. Background technique [0002] Aflatoxins are a class of mycotoxins produced by Aspergillus flavus or Aspergillus parasiticus, which are highly toxic and strong carcinogens. They were classified as Class I carcinogens by the Cancer Research Institute of the World Health Organization (WHO) in 1993. At present, there are more than 20 kinds of aflatoxins identified, and the aflatoxins that pollute grain mainly include aflatoxins B1, B2, G1, G2 and M1. The danger of aflatoxin is that it can damage the liver tissue of humans and animals, and in severe cases, it can lead to liver cancer and even death from acute poisoning. Among naturally polluted grains, aflatoxin B1 is the most toxic, the most abundant, and the most carcinogenic. Aflatoxin B1 can be detected in corn, peanuts, peanut oil, ric...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/18C12R1/66
Inventor 刘建新熊江林叶均安
Owner ZHEJIANG UNIV
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