Microbial particle preparation for aquacultural water quality purification and preparation method

A technology for water purification and aquaculture, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problem of inability to effectively decompose and remove organic sludge, the use of strains with a narrow range of salinity adaptation, and products that cannot be grown at room temperature. In order to achieve the effect of convenient industrial production, good social and economic benefits, and safe use

Active Publication Date: 2013-01-09
OCEAN UNIV OF CHINA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, although microbial water quality purifiers have been developed to a certain extent, there are still many problems. For example, microbial water quality purifiers currently on the market are generally in the form of liquid, solid powder and solid sheet. When used in water depths greater than 1 meter, It is easy to be washed away, and cannot effectively achieve the purpose of decomposing and removi

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0011] A preparation method of microbial granule preparations for water purification of aquaculture water bodies, the specific method includes the following steps: (1) Picking a loop of Bacillus subtilis strains activated by inclined planes and inoculating seed culture medium in a triangular flask, and placing 30 In a constant temperature shaker at ~37℃, shake culture at 120~150 rpm for 18~24 hours to obtain liquid first-grade Bacillus subtilis seeds with a bacterial content of at least 1×10 8 Pieces / ml; (2) Connect the above-mentioned liquid first-level seeds to the seed culture medium in the seed tank at a ratio of 1% of seed liquid: seed culture medium, and after inoculation, the temperature is 30~37℃ and the tank pressure is 0.05 MPa , The aeration ratio is 1:1, and the stirring speed is 150~200 rpm. Cultivate for 10~12 hours to obtain B. subtilis liquid secondary seed, which contains at least 2×10 8 Pcs / ml; (3) Inoculate the above secondary seeds of the bacteria liquid into ...

Example Embodiment

[0019] Example 2.

[0020] One loop of Bacillus subtilis strains activated by the slope was inoculated into an Erlenmeyer flask, (medium formula: peptone 10 g / L, beef powder 3 g / L, NaCl 5 g / L, glucose 1 g / L, use 1 mol / L HCl and 1 mol / L NaOH adjust the pH to 7.2), place it in a constant temperature shaker at 37°C and shake culture at 150 rpm for 18 hours to obtain liquid first-level seeds of Bacillus subtilis, with a concentration of 10 8 Pieces / ml; connect the liquid first-level seeds to the seed tank at a ratio of 1% of seed liquid: seed medium (medium: peptone 10 g / L, beef powder 3 g / L, NaCl 5 g / L, glucose 1 g / L, adjust the pH to 7.2 with 1 mol / L HCl and 1 mol / L NaOH, the total volume after sterilization is 70% of the seed tank volume), after inoculation, the temperature is 37℃, the tank pressure is 0.05 MPa , The aeration ratio is 1:1, and the stirring speed is 150 rpm for 10 hours. The obtained Bacillus subtilis secondary seed liquid grows neatly without contamination by bact...

Example Embodiment

[0021] Example 3.

[0022] One loop of Bacillus subtilis strains activated by the inclined plane was inoculated into an Erlenmeyer flask. (Medium formula: peptone 10 g / L, beef powder 3 g / L, NaCl 5 g / L, glucose 1 g / L, use 1 mol / L HCl and 1 mol / L NaOH adjust the pH to 7.4), place it in a constant temperature shaker at 30°C, shake culture at 120 rpm for 24 hours, and obtain liquid first-level seeds of Bacillus subtilis, with a concentration of 10 8 Pieces / ml; connect the liquid first-level seeds to the seed tank at a ratio of 1% of seed liquid: seed medium (medium: peptone 10 g / L, beef powder 3 g / L, NaCl 5 g / L, glucose 1 g / L, adjust the pH to 7.4 with 1 mol / L HCl and 1 mol / L NaOH, the total volume after sterilization is 67% of the seed tank volume), after inoculation, the temperature is 30℃, the tank pressure is 0.05 MPa , The aeration ratio is 1:1, and the agitating speed is 200 rpm. After 12 hours of cultivation, the obtained Bacillus subtilis secondary seed liquid grows neatly wi...

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Abstract

Disclosed are a microbial particle preparation for aquacultural water quality purification and a preparation method. The preparation is characterized by including utilizing zeolite as a core after steps of activation of bacillus subtilis by strains, seed liquid preparation, fermenting culturing, concentrating and the like, and then wrapping the bacillus subtilis outside the zeolite by a bacterium absorption medium to prepare gray white irregular particles with a structure of an inner layer and an outer layer. The microbial particle preparation is low in production cost, easy to store and transport and widely applicable to water quality purification of fresh water and sea water and contains more than or equal to 2X1010/g of live bacterium counts. The microbial particle preparation can be quickly sunk to water bottom after being thrown into water, the bacillus subtilises can be massively bred in water and function in water purification together with the zeolite, organic substances such as residual baits and corrosive substances can be disintegrated quickly, and water quality can be further purified by means of nitrate and nitrite in the water, diseases of bred animals can be reduced while quality and output can be improved. Further, the microbial particle preparation is simple to prepare, low in cost, stable in preparation method and convenient to produce industrially, can be stored at room temperature without refrigerating and has excellent social benefits and economic benefits.

Description

technical field [0001] The invention belongs to the technical field of aquaculture, and relates to a microbial particle preparation and a preparation method for water quality purification of aquaculture water. Background technique [0002] With the rapid development of the aquaculture industry, the scale of intensive industrial farming is expanding day by day. During the breeding process, due to the accumulation of organic substances such as feces and residual bait at the bottom of the pond, the accumulation of toxic substances such as ammonia nitrogen and hydrogen sulfide in the water caused a large number of morbidity and death of farmed animals. Antibiotics are currently the main drugs used to prevent and control farmed animal diseases. The extensive use of antibiotics not only interferes with the reproduction of probiotic flora, causes microecological imbalance, but also enhances the drug resistance of pathogens. In order to protect the ecological environment and human ...

Claims

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Application Information

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IPC IPC(8): C12N11/14C02F3/34C12R1/125C02F1/28C02F103/08
Inventor 刘成圣陈西广党奇峰高辉郭孝波范冰
Owner OCEAN UNIV OF CHINA
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