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Method for producing amino acid liquid fertilizer by processing wastewater via tilapias

A liquid fertilizer and waste water processing technology, which is applied in the direction of sewage/sludge fertilizer, organic fertilizer, fertilization device, etc., can solve the problems of untimely treatment, waste of resources, pollution of the environment, etc., and achieve low production cost, small investment, and simple equipment Effect

Inactive Publication Date: 2013-11-20
HAINAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A large amount of waste water will be produced in the process of fishmeal, which contains 4-6% protein, if not treated in time, the waste water will immediately stink within 1-3 hours, producing stench and polluting the environment
If it is discharged directly, it will not only pollute the environment, but also waste resources.

Method used

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  • Method for producing amino acid liquid fertilizer by processing wastewater via tilapias

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Experimental program
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Effect test

Embodiment 1

[0018] A method for producing amino acid liquid fertilizer with tilapia processing wastewater, comprising the following steps:

[0019] (1) Culture medium preparation process

[0020] Take the processed fish residues of tilapia, grind them into surimi with a pulverizer, take the surimi and bran, mix the two thoroughly at a weight ratio of 3:1, and then put the mixture at 130°C Sterilize under high pressure steam for 14 minutes, cool to room temperature to obtain solid medium, set aside;

[0021] (2) Inoculation and cultivation process

[0022] After cultivating Aspergillus oryzae on the slant of the test tube, use sterile water to make a concentration of 1×10 8 Aspergillus oryzae spore suspension of each / ml; Get the solid medium that step (1) obtains, inoculate 1ml of Aspergillus oryzae spore suspension in every 100g of solid medium; Then under 36 ℃, solid fermentation culture 49 hours, standby;

[0023] (3) Enzymolysis process

[0024] Take 100g of the medium cultured in ...

Embodiment 2

[0029] A method for producing amino acid liquid fertilizer with tilapia processing wastewater, comprising the following steps:

[0030] (1) Culture medium preparation process

[0031] Take the processed fish residues of tilapia, grind them into surimi with a pulverizer, take the surimi and bran, and mix them well according to the ratio of 3:1 by weight, and then put the mixture at 121℃ Sterilize under high pressure steam for 15 minutes, cool to room temperature to obtain a solid medium, and set aside;

[0032] (2) Inoculation and cultivation process

[0033] After cultivating Aspergillus oryzae on the slant of the test tube, use sterile water to make a concentration of 1×10 8 Aspergillus oryzae spore suspension of each / ml; Get the solid medium that step (1) obtains, inoculate 1ml of Aspergillus oryzae spore suspension in every 100g of solid medium; Then under 37 ℃, solid fermentation culture 48 hours, standby;

[0034] (3) Enzymolysis process

[0035] Take 100g of the medi...

Embodiment 3

[0040] A method for producing amino acid liquid fertilizer with tilapia processing wastewater, comprising the following steps:

[0041] (1) Culture medium preparation process

[0042] Take the processed fish residues of tilapia, grind them into surimi with a pulverizer, take the surimi and bran, mix the two thoroughly at a weight ratio of 3:1, and then put the mixture at 120°C Sterilize under high pressure steam for 16 minutes, cool to room temperature to obtain a solid medium, and set aside;

[0043] (2) Inoculation and cultivation process

[0044] After cultivating Aspergillus oryzae on the slant of the test tube, use sterile water to make a concentration of 1×10 8 Aspergillus oryzae spore suspension of each / ml; Get the solid medium that step (1) obtains, inoculate 1ml of Aspergillus oryzae spore suspension in the solid medium of every 100g; Then under 38 ℃, solid fermentation culture 47 hours, standby;

[0045] (3) Enzymolysis process

[0046] Take 100g of the medium cu...

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Abstract

The invention discloses a method for producing an amino acid liquid fertilizer by processing wastewater via tilapias, comprising the steps of grinding tilapia wastes into minced fillet by a grinder, mixing the minced fillet with bran according to a proportion of 3:1 to prepare a solid culture medium, sterilizing the culture medium at a temperature of 121 DEG C for 15 min, inoculating 1ml of Aspergillus oryzae spore suspension with the concentration of 1*108 per ml in every 100g of culture medium after cooling, carrying out solid fermentation and culture at a temperature of 37 DEG C for 48h to obtain culture medium full of Aspergillus oryzae, grinding and adding 100g of grinded yeast into 200ml of protein wastewater, ceaselessly stirring at a temperature of 60 DEG C for 6h, carrying out filtration and 1 / 3 vacuum concentration and adding calcium and magnesium elements to form the amino acid liquid fertilizer. According to the method, the proteins in the wastewater is decomposed into amino acid by utilizing the protease generated by the Aspergillus oryzae, so that the stink source is eradicated and the influence from the wastewater to the environment is eliminated; and the amino acid liquid fertilizer is produced through blending the calcium and magnesium elements, so that the reclamation of the wastewater is realized.

Description

technical field [0001] The invention relates to a method for recycling tilapia processing wastewater, in particular to a method for preparing amino acid liquid fertilizer by using high-concentration protein wastewater generated in tilapia processing. Background technique [0002] Tilapia farming in the four southern provinces of my country (Guangdong, Hainan, Guangxi, and Fujian) accounts for nearly 90% of the country's total output. The rapid development of the aquaculture industry has driven the development of related industries such as seedlings, feed, processing, and trade. The development of the tilapia industry in my country has a strong competitive advantage. Except for a few fresh products, most of the tilapia is processed into frozen tilapia fillets and frozen whole fish and exported to the United States, Canada, the European Union, Japan and other places. [0003] my country's tilapia processing and export products mainly include frozen fillets and frozen whole fi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C05F7/00
CPCY02A40/20
Inventor 李永成
Owner HAINAN UNIV
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