Fluorescent test strip based on resonance energy transfer, and preparation method and application for fluorescent test strip

Abstract

The invention discloses a fluorescent test strip based on resonance energy transfer, and a preparation method and application for the fluorescent test strip. The fluorescent test strip comprises a bottom liner, a sample absorption mat, a combining mat, a chromatography membrane and a water-absorbent mat, wherein the sample absorption mat, the combining mat, the chromatography membrane and the water-absorbent mat are sequentially connected with one another closely and are attached to the bottom liner; antibodies marked by fluorescent receptors are attached to the combining mat; a detection line and a calibration line are arranged on the chromatography membrane; the detection line is close to the combining mat; the calibration line is close to the water-absorbent mat; antigens marked by fluorescent substances are attached to the detection line; proteins marked by fluorescent substances are attached to the calibration line; the antigens marked by the fluorescent substances and the antibodies marked by the fluorescent receptors can be combined by specificity of antigen and antibody reaction; and the proteins marked by the fluorescent substances do not react with the antibodies marked by the fluorescent receptors. The fluorescent test strip has the advantages of safety in operation, simplicity, convenience, sensitivity, low cost, speediness and the like, is wide in application range, and can be used for single-item detection or quick detection items such as multiple-item detection.

Description

technical field [0001] The invention relates to the field of fluorescence immunochromatography detection, belongs to a kind of dry chemical detection method, and particularly relates to a fluorescent test strip based on energy resonance transfer, its preparation method and application. Background technique [0002] Immunological detection methods are a series of experimental methods designed to detect antigens, antibodies, immune cells and their secreted cytokines and small molecules by applying immunological theory. In immunological detection, radioimmunoassay, enzyme-linked immunosorbent assay, immunoelectrochemical analysis technology, colloidal gold immunochromatography and other methods are mainly used at home and abroad to achieve quantitative or qualitative detection. But these methods all have different degrees of disadvantages: [0003] ①Radioimmunoassay and enzyme-linked immunosorbent assay have high sensitivity, but they need large-scale supporting equipment, hav...

AI Technical Summary

Problems solved by technology

[0003] ① Radioimmunoassay and enzyme-linked immunosorbent assay have high sensitivity, but they need large-scale supporting equipment, have certain requirements for the detection environment, and the detection time is long, so it is not suitable for promotion at the grassroots level

The signal amplification ability of a single fluorescent dye is limited, and fluorescence quenching is prone to occur under light conditions, which affects the sensitivity and stability of the test strip

Lanthanides have relatively good stability, but the quantum yield is low, which affects the sensitivity of the test strip

Quantum dots have the characteristics of wide excitation spectrum, narrow emission spectrum, large Stokes shift and high stability, but quantum dots also have some shortcomings: quantum dots have medium brightness and high price, which affect the sensitivity of test strips and increase The cost of research and development and production of test strips, and quantum dots contain heavy metal elements, which have certain toxicity

However, fluorescent latex microspheres also have disadvantages: Compared with colloidal gold particles, fluorescent latex microspheres have higher production costs and are more difficult to produce. Compared with colloidal gold immunochromatography test strips, immunochromatography based on fluorescent latex microspheres The research on the release and running of the test strips on the test strips, the stability of the results, etc. is not very mature

According to the different detection objects and antigens and antibodies used, test strips have different requirements for the particle size and fluorescence spectrum of fluorescent latex microspheres, and except for some large foreign companies, it is difficult for domestic fluorescent latex microsphere production companies to provide various Fluorescent Spectrum and Particle Size of Fluorescent Microspheres

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