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A method for extracting guanosine from fermentation broth

An extraction method and fermentation broth technology are applied in the field of guanosine extraction, which can solve the problems of low guanosine yield and high guanosine content, and achieve the effects of improving guanosine yield, shortening extraction process and low process cost.

Active Publication Date: 2016-02-24
TONGLIAO MEIHUA BIOLOGICAL SCI TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the guanosine content in the primary mother liquor discharged by the existing method for purifying and separating guanosine is relatively high, resulting in a very low yield of guanosine

Method used

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  • A method for extracting guanosine from fermentation broth
  • A method for extracting guanosine from fermentation broth
  • A method for extracting guanosine from fermentation broth

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] In order to further understand the present invention, the present invention will be described in detail below in conjunction with examples. Embodiment 1: Utilize the method for extracting guanosine from fermentation broth described in the present invention to extract guanosine

[0047] Take 60m 3 The filtrate with a guanosine content of 25g / L after being treated by a ceramic membrane, with 21m 3 / h flow rate upper liquid, 711 resin volume is 22m 3 , and then use 60m 3 3% sodium hydroxide and 0.5% sodium chloride solution analysis, get 38m 3 , a guanosine content of 36.7g / L of the ionotropic fluid.

[0048] Adjust the pH of the ionotrans solution to 7.0 with hydrochloric acid, cool and incubate the crystals for 4 hours, and plate and frame filter to obtain 2.02 tons of crude guanosine with a content of 66% guanosine and a water content of 30%. Add crude guanosine to 44m 3 Dissolve in deionized water at 90°C, the solution transmits 85% of the light, slowly cool down...

Embodiment 2

[0048] Adjust the pH of the ionotrans solution to 7.0 with hydrochloric acid, cool and incubate the crystals for 4 hours, and plate and frame filter to obtain 2.02 tons of crude guanosine with a content of 66% guanosine and a water content of 30%. Add crude guanosine to 44m 3 Dissolve in deionized water at 90°C, the solution transmits 85% of the light, slowly cool down and cool the crystal for 4 hours, and then press filter to obtain 1.33 tons of finished guanosine. The yield of the ceramic membrane is 96%, and the yield of the product is 85%. , the content of guanosine in the finished product is 99.6%, the light transmission rate of the acid method is 95.8%, and the light transmission rate of the alkali method is 96.9%. Embodiment 2: The method for extracting guanosine from the fermentation broth according to the present invention extracts guanosine

[0049] Take 70L of the filtrate with a guanosine content of 30g / L after being treated by a ceramic membrane, top it with a fl...

Embodiment 3

[0051] Embodiment 3: Existing method for extracting guanosine extracts guanosine

[0052] Take 70L of the filtrate with a guanosine content of 30g / L after being treated with a ceramic membrane, adjust the acidity to 7.0-7.5 with hydrochloric acid, cool to 5-15°C and incubate for crystallization for 4 hours, then plate and frame filter to obtain 3045g of a crude product with a guanosine content of 60 %, water 37.3%, add 10L deionized water to dissolve the crude guanosine at 90℃, the light transmission of the solution is 67.1%, add flocculant to the solution for flocculation, then add 30% pure guanosine to decolorize with activated carbon for 1 hour, press filter, cool Cultivate the crystal at 5-15°C for 4 hours to obtain 1638g of finished guanosine, the yield of ceramic membrane is 97%, the yield of product is 75.1%, the content of guanosine in finished product is 98.6%, the light transmission of acid method is 95.4%, and the light transmission of alkali method is 96.6%.

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Abstract

The invention belongs to the technical field of fermentation, and discloses a method for extracting vernine from fermentation liquor. The method comprises the steps of filtering vernine fermentation liquor, carrying out ion exchange on the obtained filtrate by using anion exchange resin, resolving by using the mixed liquor of sodium hydroxide and sodium chloride to obtain ion exchange liquor, adjusting the pH to 7.0-7.5, cooling for crystallization, separating to obtain a vernine crude product, and carrying out re-crystallization, separation and drying. According to the method provided by the invention, the vernine fermentation liquor is directly placed on an anion exchange resin column after passing through a filtration and distillation body to selectively adsorb most of vernine in the filtrate and remove vast pigments, other proteins and the like in the filtrate, so that vernine in the filtrate is prevented from being discharged along with the mother liquor to cause vernine loss and then the vernine yield is improved. Through resolving adsorbed vernine by using the mixed liquor of sodium hydroxide and sodium chloride, the obtained ion exchange liquor is clear in color and less in impurity; and direct crystallization can be carried out to obtain vernine, so that the step of protein removal via flocculation and the step of discoloring are avoided, the extraction flow is shortened and the vernine loss is decreased.

Description

technical field [0001] The invention belongs to the technical field of fermentation, and in particular relates to a method for extracting guanosine from fermentation broth. Background technique [0002] Guanosine (Guanosine) is also known as 9-β-D-ribofuranosylguanine, referred to as Guanosine (GR), and its English name is Guanosine. Guanosine is a kind of purine nucleoside, which is composed of guanine and ribose (ribose furanose), which are connected by β-N9-glycosidic bonds. Guanosine is a white crystalline powder, the dihydrate is a needle-like crystal, dehydrated at 110°C, and the molecular formula is C 10 h 13 N 5 o 5 , relative molecular mass 283.24, melting point 240°C (decomposition), specific rotation -60°, soluble in dilute mineral acid, hot acetic acid, dilute alkali (1g / 33mL), boiling water, slightly soluble in cold water (1g / 1320mL), Insoluble in alcohol, ether, chloroform and benzene, its structural formula is as follows: [0003] [0004] Guanosine h...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H19/167C07H1/06
Inventor 彭芳菊王海雷孟祥杰刘康乐龚华
Owner TONGLIAO MEIHUA BIOLOGICAL SCI TECH CO LTD