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Method for producing 2-keto-D-gluconic acid by using fermentation method

A technology of gluconic acid and fermentation method, which is applied in the field of fermentation production of 2-keto-D-gluconic acid, can solve the problems of high energy consumption for cooling, susceptibility to bacteriophage infection, affecting production, etc., and achieves low energy consumption for cooling, controllable Easy, high-converting effects

Inactive Publication Date: 2013-02-13
SHANGHAI ADVANCED RES INST CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The fermentation temperature of bacteria such as Pseudomonas, Serratia marcescens and Alcaligenes ketogenes is generally 28°C, which makes the energy consumption for cooling higher in production; at the same time, Pseudomonas Bacteria are susceptible to phage infection, affecting production

Method used

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  • Method for producing 2-keto-D-gluconic acid by using fermentation method
  • Method for producing 2-keto-D-gluconic acid by using fermentation method
  • Method for producing 2-keto-D-gluconic acid by using fermentation method

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Embodiment 1

[0043] Klebsiella pneumoniae CGMCC1.6366budA eliminated by the 2,3-butanediol synthesis pathway constructed above - Fermenting glucose to produce 2-keto-D-gluconic acid, the steps are as follows:

[0044] 1) Preparation of culture medium

[0045] Glucose 50g / L, corn steep liquor 8g / L, yeast powder 5g / L, peptone 5g / L, sodium chloride 7g / L, mixed with tap water, then packed into 250mL Erlenmeyer flasks with a liquid volume of 50mL, each Add 0.5 g of calcium carbonate to the bottle and sterilize it. All reagents used were domestic commercial products.

[0046] 2) Inoculation of microorganisms and aerobic culture

[0047] Insert Klebsiella pneumoniae CGMCC1.6366-budA into each bottle of culture medium - One inoculation loop, aerobic culture on a shaking table at 37°C, with a rotation speed of 200 rpm, culture and fermentation for 48 hours, using added calcium carbonate to control the pH of the fermentation process, and the pH of the fermentation process gradually decreased to ...

Embodiment 2

[0050] Klebsiella pneumoniae CGMCC1.6366-budA eliminated by the 2,3-butanediol synthesis pathway constructed above - Fermenting glucose to produce 2-keto-D-gluconic acid, the steps are as follows:

[0051] 1) Prepare seed medium

[0052] Glucose 30g / L, corn steep liquor 8g / L, yeast powder 5g / L, peptone 5g / L, sodium chloride 7g / L, mixed with tap water and prepared, then packed into 250mL Erlenmeyer flasks with a liquid volume of 50mL, and Sterilize.

[0053] 2) Preparation of fermentation medium

[0054] Glucose 50g / L, corn steep liquor 8g / L, yeast powder 5g / L, peptone 5g / L, sodium chloride 7g / L, mixed with tap water, 4L fermentation medium was put into a 5L fermenter, Sterilize.

[0055] 3) Seed culture

[0056] Insert Klebsiella pneumoniae CGMCC1.6366-budA into each bottle of seed medium - One inoculation loop was aerobically cultured on a shaker at 37°C with a rotational speed of 200 rpm for 12 hours.

[0057] 4) Fermentation culture

[0058] Put 100ml of seed cultur...

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Abstract

The invention discloses a method for producing 2-keto-D-gluconic acid by using a fermentation method, which is used for producing the 2-keto-D-gluconic acid by using Lebsiella pneumoniae with a lost 2,3-butanediol synthetic route as a production strain and glucose as the raw material through controlling a fermentation liquor to be acidic under an aerobic fermentation condition. The method has the following characteristics of relatively high transformation rate, easily-controlled fermentation process and relatively low temperature reducing energy consumption in a fermentation process.

Description

technical field [0001] The invention relates to a method for producing 2-keto-D-gluconic acid, in particular to a method for producing 2-keto-D-gluconic acid by fermentation. Background technique [0002] 2-Keto-D-gluconic acid, a glucose oxidation product, is an organic acid currently produced on a large scale, which can be used as a food additive, cement plasticizer, detergent, etc. At present, 2-keto-D-gluconic acid is mainly used in the synthesis of D-isoascorbic acid. D-erythorbic acid is close to L-ascorbic acid (vitamin C) in nature, has antioxidant and ascorbic acid effects, and is widely used as a food additive to replace L-ascorbic acid. [0003] There are three main production methods of 2-keto-D-gluconic acid, including: enzymatic synthesis, chemical synthesis and fermentation production. Fermentative production of 2-keto-D-gluconic acid mainly uses bacteria of the genus Pseudomonas, Serratia marcescens, Alcaligenes ketogenes and other bacteria as production st...

Claims

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Application Information

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IPC IPC(8): C12P7/58C12N15/09C12R1/22
Inventor 郝健魏东柳鹏福史吉平姜标
Owner SHANGHAI ADVANCED RES INST CHINESE ACADEMY OF SCI
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