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Quantum dot-organic dye compound closed/open type cadmium ion ratio fluorescent probe and preparation method thereof

A ratiometric fluorescent probe and organic dye technology, applied in the field of preparation of off/on cadmium ion ratiometric fluorescent probes, can solve the problems of poor selectivity and low sensitivity, and achieve the effects of low cost, excellent luminescence performance and reduced interference

Inactive Publication Date: 2013-02-20
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Using strategies such as QDs, ratiometric fluorescence method, and fluorescence "off / on" model can help solve the problems of low sensitivity and poor selectivity in detection, which are also key technical issues to be solved urgently
[0006] So far, no Cd based on quantum dot-organic dye complex 2+ Fluorescent Probe, Quantum Dot-Based Dual Emission Ratio-Fluorescent Cd 2+ Probe, quantum dot based fluorescent "off / on" model Cd 2+ Reports about probes

Method used

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  • Quantum dot-organic dye compound closed/open type cadmium ion ratio fluorescent probe and preparation method thereof
  • Quantum dot-organic dye compound closed/open type cadmium ion ratio fluorescent probe and preparation method thereof
  • Quantum dot-organic dye compound closed/open type cadmium ion ratio fluorescent probe and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The preparation process of CdTe-FITC complex off / on type cadmium ion ratio fluorescent probe is as follows: figure 1 As shown, the detailed preparation steps are as follows: First, 40 nM CdTe QDs (maximum fluorescence emission peak wavelength lambda 595nm) solution, weigh a certain amount of FITC ( lambda =520nm) was dissolved in ultrapure water, and the concentration was adjusted to 80 nM. Then, under room temperature and slow magnetic stirring, FITC was added dropwise to the QDs solution to form a FITC-QDs complex, in which the concentrations of QDs and FITC were adjusted to 30 nM and 60 nM, respectively. Next, a standard concentration of S 2- solution, added dropwise to the complex system, to obtain a series of different S 2- Concentration of complex samples, the QDs and FITC concentrations were adjusted to 20 nM and 40 nM, S 2- The concentrations were 0, 1, 2, 3, 5, 7, 10, 15 and 20 μM in sequence. The emission spectrum of each sample was measured by fluoresc...

Embodiment 2

[0029] First, 50 nM CdTe QDs were prepared in phosphate buffer (PBS, pH = 6.5) ( lambda =595nm) solution, weigh a certain amount of FITC ( lambda =520nm) was dissolved in ultrapure water, and the concentration was adjusted to 100 nM. Then, under room temperature and slow magnetic stirring, FITC was added dropwise into the QDs solution to form a FITC-QDs complex, in which the concentrations of QDs and FITC were adjusted to 40 nM and 80 nM, respectively. Next, a standard concentration of S 2- solution, added dropwise to the complex system, to obtain a series of different S 2- Concentration of complex samples, where the concentration of QDs and FITC were adjusted to 30 nM and 60 nM, S 2- The concentrations were 0, 1, 2, 3, 5, 7, 10, 15, 20 and 25 μM in sequence. The emission spectrum of each sample was measured by fluorescence spectrometry, the excitation wavelength was 470 nm, and the average value of 6 measurements was taken as the final result. Final selection containin...

Embodiment 3

[0031] First, 100 nM CdTe QDs were prepared in phosphate buffer (PBS, pH = 6) ( lambda =550nm) solution, weigh a certain amount of FITC ( lambda =520nm) was dissolved in ultrapure water, and the concentration was adjusted to 200 nM. Then, under room temperature and slow magnetic stirring, FITC was added dropwise into the QDs solution to form a FITC-QDs complex, in which the concentrations of QDs and FITC were adjusted to 80 nM and 160 nM, respectively. Next, a standard concentration of S 2- solution, added dropwise to the complex system, to obtain a series of different S 2- Concentration of complex samples, the QDs and FITC concentrations were adjusted to 60 nM and 120 nM, respectively, S 2- The concentrations were 0, 1, 2, 3, 5, 7, 10, 15, 20, 25 and 30 μM in sequence. The emission spectrum of each sample was measured by fluorescence spectrometry, the excitation wavelength was 470 nm, and the average value of 6 measurements was taken as the final result. Final selectio...

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Abstract

The invention discloses a quantum dot-organic dye compound closed / open type cadmium ion ratio fluorescent probe and a preparation method thereof. The preparation method comprises selecting CdTeQDs to serve as a main fluorescence source, introducing fluorescein isothiocyanate (FITC) to serve as an auxiliary (or reference) chromophore, and forming a CdTe-FITC compound dual-emitting probe in a hydrogen bonding mode. The problem that fluorescence signals of a traditional single-fluorescence probe are difficult to obtain accurately is solved. A fluorescence closed / open model is adopted, disturbance of factors except an analyte on a fluorescence enhancement reaction can be effectively reduced, the fluorescence enhancement reaction has specificity, and accordingly sensitivity and selectivity of analyte detection are improved. The preparation method is simple and low in cost, and the prepared product can perform accurate, sensitive, simple, convenient and rapid detection on Cd2+ in complex systems and biological samples, has important reference values for developing high-quality fluorescence probes of other types and provides a new developing direction for research in the fields of biological detection and chemical analysis and the like.

Description

technical field [0001] The invention relates to the field of analysis and detection, in particular to a preparation method of an off / on cadmium ion ratio fluorescent probe. Background technique [0002] Wastes containing heavy metal ions seriously pollute the environment on which organisms depend and pose a huge threat to human health, especially cadmium ions (Cd 2+ ) is widely used in many industrial processes, leading to the emergence of high-concentration cadmium-containing wastes in soil, water, food and other fields. This pollution problem has aroused widespread concern. In addition, Cd 2+ It can also be accumulated in the kidneys, liver, lungs and other organs, causing a series of acute and chronic hazards to people's health. Therefore, to develop a simple, fast, cheap and efficient Cd 2+ Evaluation techniques are essential. In recent years, instrumental analysis techniques such as inductively coupled plasma mass spectrometry, particle-induced X-ray emission, atomi...

Claims

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Application Information

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IPC IPC(8): C09K11/88C09K11/06G01N21/64
Inventor 安学勤桂日军黄文学龚俊
Owner EAST CHINA UNIV OF SCI & TECH
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