Endoglucanase coding gene, recombinase and application

A technology of endoglucanase and coding gene, which is applied in the fields of application, genetic engineering, plant gene improvement, etc., can solve the problems that have not been reported in the research, and achieve the effect of adapting to a wide temperature range

Inactive Publication Date: 2013-02-20
XUZHOU UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies on cellulose-degrading bacteria are mostly Bacillus s

Method used

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  • Endoglucanase coding gene, recombinase and application
  • Endoglucanase coding gene, recombinase and application
  • Endoglucanase coding gene, recombinase and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0028] 1. Main materials

[0029] (1) culture medium

[0030] LB liquid medium: Each L medium contains 10g of peptone, 5g of yeast powder, and 10g of NaCl;

[0031] LB solid medium: Each L medium contains 10g of peptone, 5g of yeast powder, 10g of NaCl and 12g of agar powder;

[0032] Medium for hyaline circle production: Each 1L medium contains 10g of sodium carboxymethylcellulose, 10g of peptone, 5g of yeast powder, KH 2 PO 4 1g, MgSO 4 0.2g, NaCl 10g, glucose 2g, agar powder 12g;

[0033] (2) Experimental equipment

[0034] YXQ-LS-75 Vertical Pressure Steam Sterilizer——Shanghai Boxun Industrial Co., Ltd. Medical Equipment Factory

[0035] PCR Amplifier——ABI Company, USA

[0036] FA2004 Electronic Balance——Shanghai Yueping Scientific Instrument Co., Ltd.

[0037] DNP-9162 Electric Heating Constant Temperature Incubator——Shanghai Jinghong Experimental Equipment Co., Ltd.

[0038] YXJ-2 high-speed centrifuge——Changzhou Guohua Electric Co., Ltd.

[0039] Sigma3-16pk...

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Abstract

The invention discloses an endoglucanase coding gene, a recombinase and application, belonging to the technical field of biology. The sequence of the endoglucanase coding gene is shown as SEQ ID NO.1; and the sequence of the recombinase of the endoglucanase is shown as SEQ ID NO.2. Experiment proves that the recombinant endoglucanase provided by the invention has high carboxymethyl cellulose enzyme activity (up to 2245U/mL) and the transparent circle diameter of a colony is 30 times larger than that of the initial strain. The endoglucanase coding gene and the recombinase provided by the invention can be widely used in the degradation of celluloses.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to an endoglucanase coding gene, a recombinant enzyme and applications. Background technique [0002] Cellulose is the most abundant carbohydrate resource in the biosphere, accounting for 35%-50% of the dry weight of plants and widely distributed. Cellulose is a long-chain macromolecule, which is connected by glucosides through β-1,4 glycosidic bonds. The molecular weight is generally 50,000-2,500,000, which is equivalent to 300-15,000 glucose units. It is an important storage form of glucose, but Due to the difficulty of degradation, the utilization rate of cellulose resources is very low. Cellulose macromolecules can be degraded by cellulase. Cellulase is an enzyme system. According to its catalytic reaction function, cellulose can be divided into endoglucanase (endo-1,4-β-D-glucanase, EC3.2.1.4, namely C1 enzyme ), exoglucanase (1,4-β-D-glucan cellobilhydrolase or exo-1,4-β-D-glucan...

Claims

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Application Information

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IPC IPC(8): C12N15/56C12N9/42C12N15/70C12N1/21C12P19/14C12R1/19
Inventor 侯进慧刘彤陈宏伟王富威
Owner XUZHOU UNIV OF TECH
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