Collagen extracted from black butterflyfish skins with scales and application of collagen
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A fish skin and collagen technology, applied in application, food preparation, food science and other directions, to achieve the effect of cost saving, good taste and good adhesion performance
Inactive Publication Date: 2013-02-20
SHANDONG MEIJIA GROUP
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Problems solved by technology
[0009] In order to solve the singleness and low extraction rate of the above methods for extracting collagen from fish skin and fish scales, there is no record of extracting collagen from black flounder skin and fish scales , the present invention provides a collagen extracted from the skin of black flounder with scales
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Embodiment 1
[0026] A collagen extracted from the skin of black flounder with scales obtained by the following steps:
[0027] After cleaning the skin of the black flounder with scales, chop it into mince, soak it in sodium hydroxide solution, filter it, wash it until it is neutral, filter it, extract it with a compound enzyme, the compound enzyme is pepsin, papain and Alcalase compound protease according to the activity The total activity is 0.04IU / g, the extraction time is 5 hours, the temperature is 40°C, the pH is 4, and the solid-liquid ratio is 1:15.
[0028] The skin of black flounder with scales that has been chopped into mince is soaked in 10 times the volume of 0.1M sodium hydroxide for 12 hours, and other concentrations can also be used.
[0029] The extraction rate was determined to be 88.8%.
Embodiment 2
[0031] A collagen extracted from the skin of black flounder with scales obtained by the following steps:
[0032] After cleaning the skin of the black flounder with scales, chop it into mince, soak it in sodium hydroxide solution, filter it, wash it until it is neutral, filter it, extract it with a compound enzyme, the compound enzyme is pepsin, papain and Alcalase compound protease according to the activity The total activity is 0.08IU / g, the extraction time is 3 hours, the temperature is 60°C, the pH is 8, and the solid-liquid ratio is 1:8.
[0033] The extraction rate was determined to be 90.7%.
Embodiment 3
[0035] A collagen extracted from the skin of black flounder with scales obtained by the following steps:
[0036] After cleaning the skin of the black flounder with scales, chop it into mince, soak it in sodium hydroxide solution, filter it, wash it until it is neutral, filter it, extract it with a compound enzyme, the compound enzyme is pepsin, papain and Alcalase compound protease according to the activity The total activity is 0.06IU / g, the extraction time is 4 hours, the temperature is 50°C, the pH is 6, and the solid-liquid ratio is 1:12.
[0037] The extraction rate was determined to be 92.0%.
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Abstract
The invention relates to the technical field of collagen extraction from living bodies and particularly relates to collagen extracted from black butterflyfish skins with scales. The black butterflyfish skins with the scales are washed and then cut to be in a minced shape, the minced skins are immersed in sodium hydroxide, then sequentially filtered, washed to be neutral, filtered and extracted by using compound enzyme, the compound enzyme is composited by pepsin, papain and Alcalase compound protease according to an activity ratio of 1:1:2, the total activity is 0.04-0.08IU / g, the extraction time is 3-5 hours, the temperatures are between 40 DEG C and 60 DEG C, the power of hydrogen (PH) is 4-8, and the feed liquid ratio is 1: (8-15). The collagen is used in an adhesive in production of boneless fish. The compound enzyme is used for extracting the collagen from the black butterflyfish skins with the scales, the extraction rate is guaranteed, and simultaneously, the viscosity of the collagen is high; the collagen is used in the adhesive in production of the boneless fish and is ecological, environment-friendly and cost-saving; and the extracted collagen is used in the food adhesive, is safe and good in adhesion performances and free of peculiar smell and tastes good.
Description
[0001] technical field [0002] The invention relates to the technical field of collagen extraction in organisms, in particular to a collagen extracted from the skin of black flounder with scales, and also relates to the application of the collagen. [0003] Background technique [0004] Collagen is an important functional protein, which is closely related to cell proliferation, differentiation, movement, immunity, joint lubrication, calcification, wound healing, organ support, body protection, etc. It has now attracted widespread attention. Due to its special biological functions, its extracts have been widely used in many fields such as medicine, food, daily chemical industry, biosynthesis, etc., such as manufacturing medicinal capsules, surgical materials, edible gelatin, photographic gelatin, cosmetics, etc. [0005] Collagen, gelatin, and collagen (polypeptide) are three confusing terms. Collagen refers to a structural protein naturally present in organisms, containing...
Claims
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Application Information
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