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Co-production method for extracting bioactive substances from pig blood

A technology of biologically active substances and pig blood, applied in the field of separation and extraction of biologically active substances, can solve problems such as unsuitable for industrialized production, unqualified product yield and quality, complicated control of key variables and time, etc. The effect of high utilization

Inactive Publication Date: 2013-02-27
HUAIBEI ENBI FEED
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In the deep processing of pig blood, modern biotechnology such as protease hydrolysis technology, biofilm technology, homogeneous cell wall breaking technology, anticoagulant technology, extraction technology, etc. must be used comprehensively, and these technical treatments must be completed in a complex organic environment. The control of key variables and time in the reaction environment is relatively complicated, and improper control of any link will lead to unqualified product yield or quality
The mastery of these core process technologies requires long-term exploration and accumulation, and the integration and optimization of the production line also requires continuous exploration, and if a single substance is extracted from pig blood, the utilization rate of pig blood is low, and it is not suitable for industrial production

Method used

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  • Co-production method for extracting bioactive substances from pig blood

Examples

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Embodiment 1

[0023] Example 1: A co-production method for extracting biologically active substances from pig blood, including the extraction of immunoglobulin G, the extraction of heme iron and protein peptides, and the extraction of SOD.

[0024] 1. Extraction of immunoglobulin G:

[0025] After 100kg of anticoagulant blood is separated, add 55kg of plasma to 55kg of PBS buffer for dilution, stir slowly and add 6.0kg of ammonium sulfate solid, the saturation reaches 20-25%, and adjust the pH of the plasma to 5.5, let it stand at room temperature for 30 minutes, and then collect it by pressure filtration 100L of supernatant; stir slowly in the supernatant and add 19.4kg of ammonium sulfate solid until the saturation reaches 35-40%, adjust the pH to 7.2, let stand for 30min, and press filter to collect the precipitate; add 50kg of pure water to dissolve the precipitate, add sulfuric acid 9.7kg of ammonium crystals, adjust the pH to 7.2, and then press filter to collect the precipitate; repe...

Embodiment 2

[0031] 1. Extraction of immunoglobulin G:

[0032]After separation of 5000kg anticoagulant original blood, add 2750kg of plasma to 2750kg of PBS buffer for dilution, stir slowly and add 320.0kg of ammonium sulfate solid, and adjust the pH of the plasma to 5.6. Slowly add 980kg of ammonium sulfate solid, adjust the pH to 7.4 and let it stand for 30 minutes to collect the precipitate by pressure filtration; add 2500 kg of pure water to dissolve the precipitate, add 490 kg of ammonium sulfate crystals to adjust the pH to 7.2, and then process for 30 minutes to collect the precipitate by pressure filtration; repeat the above One process twice, add 2500kg of pure water to dissolve the precipitate, then conduct ultrafiltration to concentrate and desalinate, the operating pressure is 0.15MPa, concentrate until no SO is detected 4 2- Low-temperature spray drying was carried out. The inlet temperature of the spray drying was 160°C, and the outlet temperature was 80°C. A total of 3.45k...

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Abstract

The invention discloses a co-production method for extracting bioactive substances from pig blood. The co-production method comprises immune globulin G extraction, heme iron and protein peptide extraction and superoxide dismutase (SOD) extraction. The immune globulin G extraction orderly comprises carrying out salting-out to remove a fibrous protein and to obtain blood serum and repeating salting-out extraction three times to obtain immune globulin G crude extract. The heme iron and protein peptide extraction comprises breaking blood cells in a blood cell liquid, carrying out press filtration, respectively collecting a supernatant and precipitates, drying the precipitates at a low temperature to obtain heme iron powder, carrying out decoloration of the supernatant by activated carbon, carrying out spray drying to obtain a protein peptide finished product. The SOD extraction comprises collecting residues obtained by the blood cell breaking, carrying out extraction and washing, carrying out press filtration, collecting a supernatant which is a crude enzyme liquid, carrying out separation by acetone, collecting precipitates, heating, washing by acetone, carrying out press filtration, collecting precipitates, washing the precipitates by ethanol, and carrying out freeze drying to obtain SOD. The co-production method fully utilizes raw materials, realizes extraction of four high-additional value products from pig blood, has a high equipment utilization rate and simple extraction processes, and is suitable for industrial production.

Description

technical field [0001] The invention belongs to the technical field of separation and extraction of biologically active substances, and in particular relates to a coproduction method for extracting biologically active substances from pig blood. Background technique [0002] Pig blood (pig red), also known as liquid meat, blood tofu and blood flower, is rich in vitamins, protein, iron, phosphorus, calcium, niacin and other nutrients. my country's pig blood resources are very rich, but the depth and breadth of the utilization of pig blood resources in my country are very low at present, mainly used for the production of pig plasma protein powder and blood globulin powder for feed, and have not been well utilized to realize the use of pig blood. "Turning Waste into Treasure". [0003] Pig blood is composed of plasma and blood cells, including red blood cells, white blood cells, and platelets. Take a certain amount of blood and mix it with an anticoagulant, put it in a test tub...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/06C07D487/22C12N9/08C12P21/06
Inventor 邹国庆吴明文卢进峰徐宝海姚远新
Owner HUAIBEI ENBI FEED
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