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Florigen-activating complex

A florigen and compound technology, applied in compound screening, angiosperms/flowering plants, plant peptides, etc., can solve problems such as unclear mechanism of florigen

Active Publication Date: 2013-02-27
NARA INSTITUTE OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Although there are many research reports on florigens, the mechanism of action of florigens in flowering induction is still unclear

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0219] (Example 1) Confirmation of the interaction of three proteins

[0220] (1) The interaction of the three proteins was confirmed by in vitro GST pull-down experiments.

[0221] Restriction enzyme treatment was performed on the plasmid inserted with the cDNA encoding the full-length Hd3a (1-179 residues) shown in Sequence No. 1 and the cDNA encoding the full-length GF14c (1-256 residues) shown in Sequence No. 2, to make The fragment treated with the restriction enzyme was fused with the polynucleotide encoding GST and introduced into the vector. This vector was introduced into Escherichia coli, and GST fusion GF14c protein or GST fusion Hd3a protein was expressed in Escherichia coli. After the Escherichia coli is cultured, the Escherichia coli is centrifuged and recovered, and the GST fusion GF14c protein or the GST fusion Hd3a protein is separated and purified from the solution.

[0222] 10 nmol of the isolated and purified GST-fused GF14c protein or GST-fused Hd3a prot...

Embodiment 2

[0226] (Example 2) Preparation of crystals of florigen activation complex

[0227] For the cDNA inserted with 6-170 residues in the full-length Hd3a (1-179 residues) shown in the coding sequence number 1, and 1-235 residues in the full-length GF14c (1-256 residues) shown in the coding sequence number 2 The cDNA plasmid of the residue was subjected to restriction enzyme treatment, and the fragment treated with the restriction enzyme was fused with a polynucleotide encoding GST to introduce it into a vector, and the vector was introduced into Escherichia coli. GST-fused Hd3a protein and GST-fused GF14c protein were expressed in Escherichia coli. After the E. coli was cultured, the E. coli was centrifuged and recovered, and the protein was purified from the lysed solution. Thereafter, the protein was analyzed by SDS-polyacrylamide electrophoresis (SDS-PAGE), and the purity of the protein was found to be 95% or more. A polypeptide encoding residues 187 to 195 of the full-length ...

Embodiment 3

[0235] (Example 3) Analysis of the X-ray structure of the crystal of the florigen-activated complex

[0236] The X-ray diffraction images of the obtained crystals were measured using a CCD detector at the BL5A beamline of Photon Factor, a radiological science research facility. The atomic coordinates of the florigen activation complex were obtained from the obtained diffraction images.

[0237] The X-ray diffraction data were collected, and the indexing of each diffraction spot and the calculation of the diffraction intensity were performed. The phase angle was determined from the obtained diffraction intensities and a search model using the molecular replacement method. Using the diffraction intensities and phase angles of these diffraction spots, an electron density map was derived by inverse Fourier transform. Atomic coordinates were constructed from the resulting electron density maps.

[0238] Specifically, the obtained data were processed using HKL2000 and scaled by S...

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Abstract

In order to provide crystals of a florigen-activating complex obtained by binding a florigen, a 14-3-3 protein and a bZip transcription factor, and to control the flowering of plants by using said crystals to regulate the mechanisms of interaction between these substances, Hd3a is bound to FD1 via GF14c to form a florigen-activating complex, crystals are prepared from the florigen-activating complex, steric structural information is obtained using the crystals of the florigen-activating complex, and the steric structural information is used to control the interaction mechanism of the florigen and the like in order to thereby control the flowering of a plant.

Description

technical field [0001] The present invention relates to the crystal of the florigen activation complex formed by the combination of the 14-3-3 protein and the bZIP transcription factor, and the three-dimensional structure information of the florigen activation complex obtained from the crystal and the florigen Functional utilization of the activation complex. [0002] This application claims the priority of Japanese application Japanese Patent Application No. 2010-061562 incorporated herein by reference. Background technique [0003] Regulation of flowering of plants in various environments is considered to be effective in realizing stable food production or construction of a recycling-type economic and social system that effectively utilizes plant biomass and the like. In order to construct technologies to regulate flowering in plants, the molecular basis of information transmission of plant environmental responses needs to be clarified. [0004] As a factor for inducing ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/09A01H1/00A01H5/00C07K14/415C30B7/14C30B29/58G01N33/53G06F19/16
CPCC07K14/415A01H5/02C30B7/00C30B29/58G01N2500/00C12N15/827C12N15/00
Inventor 大木出田冈健一郎辻宽之儿岛长次郎岛本功
Owner NARA INSTITUTE OF SCIENCE AND TECHNOLOGY
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