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Bioactive polypeptide for promoting osteogenic growth and preparation method thereof

A technology for bioactive polypeptides and osteogenesis, which is applied in the field of biotechnological polypeptides and medical materials, can solve problems such as limited sources and biocompatibility limitations, and achieve the promotion of new bone formation, good biocompatibility, and promotion of osteogenesis differentiation effect

Inactive Publication Date: 2013-03-06
NORTHWESTERN POLYTECHNICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The Chinese patent with the publication number CN101496911A discloses a bone scaffold material obtained by cross-linking heparin on the decalcified bone matrix. Since it is obtained by decalcification and cross-linking of mammalian bones, the source is limited, and it is affected by its Biocompatibility Limitations

Method used

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  • Bioactive polypeptide for promoting osteogenic growth and preparation method thereof
  • Bioactive polypeptide for promoting osteogenic growth and preparation method thereof
  • Bioactive polypeptide for promoting osteogenic growth and preparation method thereof

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preparation example Construction

[0044] The preparation of the hydrogel includes the following steps: weigh ELK-8, ELK-ALK, ELK-DGR and ELK-PRG four kinds of peptide powders (mass ratio is 4:1:1:1), and dissolve them in deionized water to prepare respectively into a storage concentration of 1% w / v (10 mg / mL), then mix ELK-ALK, ELK-DGR and ELK-PRG with ELK-8 at a ratio of 5:1 to 1:5 (V:V), Sonicate for 30 minutes, and dilute the concentration to ≥0.1% with deionized water to obtain ELK-8, ELK-8+ELK-ALK mixed solution (ALK mix), ELK-8+ELK-DGR mixed solution (DGR mix), ELK- 8+ ELK-PRG mixed solution (PRG mix) four kinds of working solutions, add more than 1 times the volume of total salt ions (including Na, K, Ca, Mg and Cl) concentration ≥ 1mM to initiate self-assembly to form a hydrogel scaffold, Applied to bone tissue engineering.

[0045] In the synthetic polypeptide general formula of the present invention, repeating sequence (X 1 -X 2 -X 1 -X 2 -X 1 -X 3 -X1 -X 3 ) n The replaceable amino acids ar...

preparation Embodiment 1

[0087] Weigh 80mg of ELK-8 polypeptide powder, 20mg each of ELK-ALK, ELK-DGR, and ELK-PRG three kinds of polypeptide powders, and dissolve them in deionized water to prepare a storage concentration of 1% w / v (10mg / mL), ELK -ALK, ELK-DGR, ELK-PRG were mixed with ELK-8 at a ratio of 1:1 (V:V), ultrasonicated for 30 minutes, and diluted with deionized water to 0.2%, to obtain ELK-8, ELK-8+ ELK-ALK mixed solution (ALK mix), ELK-8+ELK-DGR mixed solution (DGR mix), ELK-8+ELK-PRG mixed solution (PRG mix) four working solutions, add 4 times the volume of 0.9% Saline triggers self-assembly to form a hydrogel scaffold.

preparation Embodiment 2

[0089] Weigh 80mg of ELK-8 polypeptide powder, 20mg each of ELK-ALK, ELK-DGR, and ELK-PRG three kinds of polypeptide powders, and dissolve them in deionized water to prepare a storage concentration of 1% w / v (10mg / mL), ELK -ALK, ELK-DGR, ELK-PRG were mixed with ELK-8 at a ratio of 1:1 (V:V), ultrasonicated for 30 minutes, and diluted with deionized water to 0.2%, to obtain ELK-8, ELK-8+ ELK-ALK mixed solution (ALK mix), ELK-8+ELK-DGR mixed solution (DGR mix), ELK-8+ELK-PRG mixed solution (PRG mix) four working solutions, add 4 times the volume of modified α -The medium induces self-assembly, forming a hydrogel scaffold.

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Abstract

The invention discloses bioactive polypeptide for promoting osteogenic growth and a preparation method thereof. The amino sequence is any one of Ac-(X1-X2-X1-X2-X1-X3-X1-X3)n-GG-ALKRQGRTLYGF-CONH2, Ac-(X1-X2-X1-X2-X1-X3-X1-X3)n-GG-DGRGDSVAYG-CONH2 and Ac-(X1-X2-X1-X2-X1-X3-X1-X3)n-G-PRGDSGYRGDS-CONH2; and the polypeptide is synthesized by a solid-phase synthesis method. Through the bioactive polypeptide disclosed by the invention, the nutritional substances, bioactive molecules and oxygen can reach the cultivated cells, the biocompatibility is good, the restriction on source is broken through, and the osteogenic growth peptide can obviously promote osteogenic differentiation and new bone formation.

Description

technical field [0001] The invention relates to a biologically active polypeptide for promoting bone growth, and belongs to the field of biotechnological polypeptides and medical materials. Background technique [0002] Regenerative medicine and tissue engineering require two key factors: scaffold materials that are non-toxic to the body and have good biocompatibility, and suitable cells that can effectively replace injured tissues without side effects, including various stem cells and progenitor cells. Therefore, selecting appropriate and effective bioactive scaffolds to stimulate and promote cell differentiation and support tissue regeneration is a key issue in tissue engineering. [0003] In 1992, Zhang Shuguang from the Massachusetts Institute of Technology accidentally discovered an ionic short peptide capable of self-assembly from yeast protein. The ionic short peptide is a self-assembly system composed of 8 to 16 amino acids and can be used as a cell culture scaffold ...

Claims

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Application Information

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IPC IPC(8): C07K7/08C07K14/00C07K1/06C07K1/04A61L27/22A61L27/52
Inventor 卢婷利陈婷马玉樊校月红李昱辉
Owner NORTHWESTERN POLYTECHNICAL UNIV
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