Synthetic method and extraction process of blue natural dye

A natural dye, blue technology, applied in the field of bioengineering, can solve the problems of complex extraction process, complex structure of synthetic dyes and high production cost

Active Publication Date: 2013-03-06
杭州唯铂莱生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Natural dyes have been used earlier, and most of them are non-toxic and environmentally friendly, but there are limitations such as limited content in nature, complicated extraction process, and high production cost.
Compared with natural dyes, synthetic dyes have the advantages of bright color, not limited by the source of raw materials, and low production cost, but the...

Method used

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  • Synthetic method and extraction process of blue natural dye
  • Synthetic method and extraction process of blue natural dye
  • Synthetic method and extraction process of blue natural dye

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0075] (2) Preparation of engineered bacteria seed culture solution

[0076] The preparation of the seed culture solution of A, engineering bacterium C

[0077] Pick a single bacterium colony from the solid medium that is preserved with engineering bacteria C, and put it in 5 mL of LB medium containing 50ug / mL kanamycin, and cultivate it in a shaker at 37°C for 12 hours at 300rpm to obtain engineering bacteria C. Seed culture solution; the percentages of the amount of each component in the solid medium to the mass of the solid medium are: tryptone 1%; yeast extract 0.5%; NaCl 1%; agar 2%; pH 7.0; after sterilization Add kanamycin 50ug / mL; the percentages of the amount of each component in the LB medium to the quality of the LB medium are: tryptone 1%; yeast extract 0.5%; NaCl 1%; pH 7.0;

[0078] B, the preparation of the seed culture solution of engineering bacterium CB

[0079] Pick a single colony from the solid medium preserved with the engineered bacteria CB and pla...

Embodiment 1

[0096] Example 1 Transformation of engineering bacteria BL21 (DE3) with recombinant plasmids

[0097] Transform the recombinant plasmid pET28a(+) / SC-indC Enter the engineering bacteria BL21 (DE3), and name the transformed engineering bacteria as C; co-transform the recombinant plasmid pET28a(+) / SC-indC and pACYCDuet-1 / SC-indB Enter the engineering bacteria BL21 (DE3), and name the transformed engineering bacteria as CB.

Embodiment 2

[0098] Example 2 Preparation of Engineering Bacteria Seed Culture Solution

[0099]Preparation of seed culture solution of A engineering bacteria C

[0100] Pick a single bacterium colony from the solid medium that is preserved with engineering bacteria C, and put it in 5 mL of LB medium containing 50ug / mL kanamycin, and cultivate it in a shaker at 37°C for 12 hours at 300rpm to obtain engineering bacteria C. Seed culture solution; the percentages of the amount of each component in the solid medium to the mass of the solid medium are: tryptone 1%; yeast extract 0.5%; NaCl 1%; agar 2%; pH 7.0; after sterilization Add kanamycin 50ug / mL; the percentages of the amount of each component in the LB medium to the quality of the LB medium are: tryptone 1%; yeast extract 0.5%; NaCl 1%; pH 7.0;

[0101] Preparation of Seed Culture Solution of B Engineering Bacteria CB

[0102] Pick a single colony from the solid medium preserved with the engineered bacteria CB and place it in 5 m...

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Abstract

A synthetic method and an extraction process of blue natural dye belong to the field of biological engineering. The purpose of the invention is to provide the synthetic method and the extraction process by using biological materials to synthesize non-toxic and environmental-friendly blue natural dye. The present invention uses purified brown chromogenic streptomycete genomic DNA as a template, uses a primer C1: 5'-aaTTAATTAAGGAGGAGCCCATatgagcgtagagaccatccc-3' and a primer C2: 5'-aaGCTAGCAAGCTTtcagtagttgggcgtcttgc-3' to was amplify indigoidine synthase gene SC-indC by a polymerase chain reaction, wherein the amplified SC-indC gene sequence is SEQ ID NO: 1. The method and the extraction process using engineering bacteria to synthesize blue natural dye indigoidine are high in yield, not restricted by sources of raw materials, easy to control the process, and low in production costs.

Description

technical field [0001] The invention belongs to the field of biological engineering. Background technique [0002] In recent years, with the advancement of science and technology and the improvement of people's living standards, the application of dyes has become more and more extensive, especially the demand for non-toxic and environmentally friendly dyes is increasing, which puts forward higher requirements for the development of the dye industry . Current dyes can be divided into two categories according to their sources: natural dyes and synthetic dyes. Natural dyes have been used earlier, and most of them are non-toxic and environmentally friendly, but there are limitations such as limited content in nature, complicated extraction process, and high production cost. Compared with natural dyes, synthetic dyes have the advantages of bright color, not limited by the source of raw materials, and low production cost, but the disadvantages are that most of the synthetic dyes...

Claims

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Application Information

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IPC IPC(8): C12N15/52C12N15/10C12N9/00C12N15/31C07K14/36C12N15/70C12N1/21C12P17/16C12R1/465
Inventor 乔楠陶福平于大禹徐富超
Owner 杭州唯铂莱生物科技有限公司
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