Method for breeding, culturing and storing plant nematodes by using sweet potato calluses

A callus and nematode technology, applied in the field of using sweet potato callus to propagate, cultivate and preserve plant-parasitic nematodes, can solve the problems of low reproductive success rate and reproduction amount, inability to cultivate and preserve for a long time, and high water content in carrots, and achieve success High efficiency, simple operation, and easier to obtain effects

Active Publication Date: 2013-03-20
嘉兴卓十生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But utilize culture medium to cultivate plant callus or fungus, then inoculate and cultivate and reproduce nematodes, the operation is complicated, and pollution is prone to occur simultaneously, resulting in low reproduction success rate and reproduction amount, directly using the callus grown from carrot slices without medium to propagate and The method of cultivating and preserving the banana perforator nematode also has defects such as that carrots have a large water content and are prone to rot with endophytic bacteria, so they cannot be cultivated and preserved for a long time

Method used

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  • Method for breeding, culturing and storing plant nematodes by using sweet potato calluses
  • Method for breeding, culturing and storing plant nematodes by using sweet potato calluses
  • Method for breeding, culturing and storing plant nematodes by using sweet potato calluses

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1 Propagation and preservation of D. destructor on sweet potato callus

[0041] Specific steps are as follows:

[0042] (1) Preparation of sweet potato callus:

[0043] Select healthy, relatively slender and regular sweet potato pieces (Guangshu 128), wash and dry them; soak them in 70% alcohol by mass for 2 minutes, then burn off the alcohol on the surface in an ultra-clean workbench, and use sterilized shavings Peel off the skin of the sweet potato with a leather knife, then cut into thin slices of about 4mm, and put the thin slices into a sterile petri dish ( figure 1 ), sealed with plastic wrap, and cultured in the dark in a constant temperature incubator at 25°C until the callus grows for later use (generally, the callus will appear in 7 days, and the tubers will be covered with potato pieces in 10-15 days) ( figure 2 ).

[0044] (2) Isolation of D. destructor

[0045] Cut the symptomatic sweet potato pieces, cut out some symptomatic sweet potato tissu...

Embodiment 2

[0052] Implementation 2 Propagation and preservation of D. tsienmus on sweet potato callus

[0053] Specific steps are as follows:

[0054] (1) See Example 1 for the preparation of sweet potato callus.

[0055] (2) Isolation of D. tesselis

[0056] The symptomatic diseased root is cut into pieces with scissors, and separated from the matrix around the diseased root using the Behmann funnel method. The method is the same as in Example 1.

[0057] (3) Disinfection, inoculation and cultivation of D.

[0058] 25 female worms were picked, and the petri dish was placed in an incubator at 18° C. for cultivation after inoculation, and other disinfection and inoculation methods were the same as in Example 1.

[0059] (4) The collection and counting of the nematodes after being cultured on the sweet potato callus for a certain period of time can be seen in Example 1 for specific methods.

[0060] After inoculating 25 D. trunchelis on the sweet potato callus and culturing for 50 days...

Embodiment 3

[0062] Example 3 Propagation and preservation of banana borer nematode on sweet potato callus

[0063] Specific steps are as follows:

[0064] (1) See Example 1 for the preparation of sweet potato callus.

[0065] (2) Isolation of banana perforator nematode

[0066] Symptomatic diseased root tissue is shredded with scissors, and separated from the matrix around the diseased root using the Behmann funnel method, the method is the same as in Example 1.

[0067] (3) Banana perforator nematode disinfection, inoculation and culture

[0068] Pick 25 female worms, and other disinfection and inoculation methods are the same as in Example 1.

[0069] (4) The collection and counting of nematodes after being cultured on the sweet potato callus for a certain period of time refer to Example 1 for specific methods.

[0070] After 25 nematodes were inoculated on the sweet potato callus and cultured for 50 days, the average number of worms reproduced was 21836±1459.2, the average number o...

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Abstract

The invention discloses a method for breeding, culturing and storing plant nematodes by using sweet potato calluses. The method is implemented through the following steps of: cleaning fresh sweet potatoes, performing surface sterilization, peeling, slicing, placing into a culture dish, and culturing in an incubator to obtain sweet potato calluses; inoculating sterilized plant nematodes onto the sweet potato calluses; placing into respective incubators of appropriate temperatures, and culturing and breeding in dark; and after culturing for a certain period of time, storing the plant nematodes and the sweet potato calluses at the temperature of 4-20 DEG C. Due to the adoption of the method, the aims of manually culturing and breeding a large quantity of plant nematodes in a simple way and storing plant nematode resources for a long time can be fulfilled.

Description

technical field [0001] The invention relates to a method for propagating, cultivating and preserving plant parasitic nematodes, in particular to a method for propagating, cultivating and preserving plant parasitic nematodes using sweet potato callus. Background technique [0002] For plant parasitic nematodes, it is necessary to study basic researches such as taxonomy, molecular biology, functional genomics, and proteomics, or to conduct basic and applied research on biology, pathology, and control methods in the laboratory and field. , Purified nematodes. However, most plant-parasitic nematodes are obligate parasitic, difficult to culture on pure medium, and can only reproduce on the corresponding parasitic plants. In practice, people often use plant tissue culture techniques to cultivate fresh plant callus, isolated roots or Some other plant organs and whole plant seedlings are then inoculated on these plant materials to propagate plant parasitic nematodes; plant nematod...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033A01H4/00
Inventor 谢辉徐春玲李宇丁莎李克梅
Owner 嘉兴卓十生物科技有限公司
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