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Surface enhanced Raman scattering tag based on gold and silver nanowire array and preparation method thereof

A technology of surface-enhanced Raman and nanowire arrays, applied in the fields of bioanalytical chemistry and nanomaterials, can solve the problems affecting the SERS enhancement ability and practical application, the stability and biocompatibility are not very ideal, and the SERS activity is easily destroyed. problems, to achieve good biocompatibility, low cost, and high reproducibility

Inactive Publication Date: 2013-03-20
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] However, the above-mentioned pure silver nanowire arrays are obtained by direct chemical reduction deposition of silver and are directly exposed to the external environment
These pure silver substrates have inherent disadvantages that the surface is prone to contamination, stability and biocompatibility are not very ideal
Therefore, when placed in air or chemical substances to be tested such as acid or salt solution, its excellent SERS activity is easily destroyed, which will seriously affect its SERS enhancement ability and practical application
In addition, the activity of biological samples immobilized on silver surfaces is easily affected by silver ions

Method used

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  • Surface enhanced Raman scattering tag based on gold and silver nanowire array and preparation method thereof
  • Surface enhanced Raman scattering tag based on gold and silver nanowire array and preparation method thereof

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preparation example Construction

[0022] A method for preparing a surface-enhanced Raman scattering label based on a gold-silver nanowire array, the method comprising the following steps:

[0023] Form a uniform array of pure silver nanowires;

[0024] Sonicate the porous alumina sheet in deionized water, then change the deionized water, repeat 3 times; immerse the porous alumina sheet in 3M sodium hydroxide solution for 3 minutes, take it out and rinse it repeatedly with deionized water for 3 minutes and blow dry with nitrogen gas;

[0025] Then immerse the pure silver nanowire substrate in the aqueous solution of chloroauric acid with a concentration of 1mM-5mM, and the reaction time is 5-20 minutes; take out the silver nanowire substrate, rinse it sideways with deionized water, and blow it dry with nitrogen. Obtain a uniform surface-enhanced Raman scattering active substrate;

[0026] Organic molecules were selected as reporter molecules, and the substrate was immersed in it to react for 20 minutes; the s...

Embodiment 1

[0029] Example 1, preparation of gold-silver nanowire array SERS marker with 4MBA as reporter molecule

[0030] The first step is to prepare pure silver nanowire arrays.

[0031] Pure silver nanowire arrays are prepared by chemically depositing silver in anodic porous alumina sheets. The pore size of the porous anodized aluminum sheet is preferably 70nm, and the reaction time is 20 hours.

[0032] The second step is to prepare the gold-silver nanowire array SERS substrate.

[0033] Sonicate the porous alumina sheet in deionized water, then change the deionized water, repeat 3 times. Then the porous alumina sheet was immersed in a 3M sodium hydroxide solution for 3 minutes, and after being taken out, it was repeatedly rinsed with deionized water for 3 times and then dried with nitrogen. The pure silver nanowire substrate was immersed in an aqueous solution of chloroauric acid with a concentration of 1 mM, and the reaction time was 10 minutes. The substrate was taken out and...

Embodiment 2

[0037] Example 2, preparation of gold-silver nanowire array SERS marker with DTNB as reporter molecule

[0038] The specific steps are roughly the same as above, the difference is that in the third step, the concentration is 10 -3 M's DTNB solution instead at a concentration of 10 -3 4MBA solution of M. The gold-silver nanowire array SERS substrate is fixed on a confocal Raman spectrometer, and when the SERS spectrum is detected, the laser source is a 633nm helium-neon laser, which is focused by reflected light, and the irradiation power on the sample is 1.2mW. The integration time is 30 seconds.

[0039] The invention has good uniformity and superhigh repeatability, and the preparation method is simple and the cost is low.

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Abstract

The invention discloses a surface enhanced Raman scattering tag based on a gold and silver nanowire array and a preparation method thereof. The tag is formed by a gold and silver double-metal nanowire array, each nanowire in the array is formed by two layers of core-shell metal, wherein the core layer is a silver nanowire, the shell layer is a gold nanowire, and organic molecules adsorbed by the outer surface of the shell layer are Raman report molecules. The method comprises the following steps that a uniform pure silver nanowire array is formed; a porous alumina film is soaked in deionized water for ultrasound, then the deionized water is replaced, and the step is repeated for three times; the porous alumina film is soaked in a sodium hydroxide solution with the concentration of 3 M for three minutes, then the porous alumina film is repeatedly washed with the deionized water for three times after being taken out, and then the porous alumina film is blow-dried with nitrogen; then a pure silver nanowire substrate is soaked in a chloroauric acid aqueous solution with the concentration of 1 mM to 5 mM; and after the pure silver nanowire substrate is taken out, the pure silver nanowire substrate is thoroughly washed with the deionized water, and then the pure silver nanowire substrate is blow-dried with the nitrogen. The surface enhanced Raman scattering tag has the advantages of good uniformity and superhigh repeatability, and the preparation method has the advantages of simplicity and low cost.

Description

technical field [0001] The invention relates to the fields of nanomaterials and bioanalytical chemistry, in particular to a surface-enhanced Raman scattering label based on a gold-silver nanowire array and a preparation method thereof. Background technique [0002] Currently, SERS (Surface Enhanced Raman Scattering) active substrates prepared using various gold, silver, and gold-silver composite nanoparticles have been widely used. The advantage of these substrates is that the SERS enhancement factor is relatively large, and at the same time, the huge enhancement factor makes it have the detection sensitivity of the submolecular layer. However, due to the random distribution of these nanoparticles on the substrate, these substrates usually perform very poorly in uniformity and repeatability. [0003] The gold-silver nanowire array is also a substrate with good SERS activity, and it also shows a huge enhancement factor and ultra-high sensitivity in SERS detection. Moreover,...

Claims

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Application Information

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IPC IPC(8): B82B1/00B82B3/00G01N21/65
Inventor 王著元何雷崔一平钟嫄
Owner SOUTHEAST UNIV
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