New process for extracting lactic acid from lactic acid fermentation liquid

A technology of lactic acid fermentation and a new process, which is applied in the separation/purification of carboxylic acid compounds, the preparation of carboxylate, and the preparation of organic compounds. It can solve the problems of low purity, low product extraction rate, and high loss rate of calcium lactate. Achieve the effect of reducing production cost, simplifying process and reducing emissions

Active Publication Date: 2013-03-20
QINGDAO KEHAI BIOLOGICAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The process has the advantages of easy control and simple equipment requirements, but the product extraction rate is low and the purity is not high
The reasons for the low extraction rate and low purity of lactic acid are as follows: major impurities such as sugars, proteins and pigments in the fermentation broth cannot be effectively removed, which significantly affects the crystallization rate and purity of calcium lactate; The loss rate of lactic acid is as high as 30%; the sugar removal in the process is insufficient, resulting in residual sugar or other reducing substances in the finished lactic acid affecting the quality of the product

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Contains glucose, yeast extract, peptone, K 2 HPO 4 , Sodium acetate, MgSO 4 ·7H 2 O, MnSO 4 ·H 2 Lactic acid bacteria were inoculated into the medium of O, calcium carbonate was added as a neutralizing agent, and the lactic acid fermentation broth was obtained by fermentation. Heat to 85°C, add calcium hydroxide to adjust pH to 11, keep stirring for 20 minutes, filter with filter cloth to remove insolubles such as calcium carbonate in the lactic acid fermentation broth to obtain feed solution 1. The flocculant polyacrylamide was added to the feed liquid 1, and after standing for 4 hours, the filtrate 2 was obtained by filtration. Then, at a temperature of 65°C, according to the mass ratio of activated carbon to feed solution 1 of 0.05:100, activated carbon was added to decolorize feed solution 1 for 40 min, and then filtrate 3 was obtained by successively filtering with plate frame and inorganic ceramic membrane. The filtrate 3 was cooled to 55° C. with a plate h...

Embodiment 2

[0029] Contains glucose, yeast extract, peptone, K 2 HPO 4 , Sodium acetate, MgSO 4 ·7H 2 O, MnSO 4 ·H 2 Lactic acid bacteria were inoculated into the medium of O, calcium carbonate was added as a neutralizing agent, and the lactic acid fermentation broth was obtained by fermentation. Heat to 80°C, add calcium hydroxide to adjust pH to 12, keep stirring for 30 minutes, filter with filter cloth to remove insolubles such as calcium carbonate in the lactic acid fermentation broth to obtain feed solution 1. The flocculant polyacrylamide was added to the feed liquid 1, and after standing for 3.5 hours, the filtrate 2 was obtained by filtration. Then, at a temperature of 70°C, according to the mass ratio of activated carbon to feed solution 1 of 0.1:100, activated carbon was added to decolorize feed solution 1 for 40 min, and then filtrate 3 was obtained by successively filtering with plate frame and inorganic ceramic membrane. The filtrate 3 was cooled to 60° C. with a plate ...

Embodiment 3

[0031] Contains glucose, yeast extract, peptone, K 2 HPO 4 , Sodium acetate, MgSO 4 ·7H 2 O, MnSO 4 ·H 2 Lactic acid bacteria were inoculated into the medium of O, calcium carbonate was added as a neutralizing agent, and the lactic acid fermentation broth was obtained by fermentation. Heat to 90°C, add calcium hydroxide to adjust pH to 10, keep stirring for 40 minutes, filter with filter cloth to remove insolubles such as calcium carbonate in the lactic acid fermentation broth to obtain feed solution 1. The flocculant polyacrylamide was added to the feed liquid 1, and after standing for 3.5 hours, the filtrate 2 was obtained by filtration. Then, under the temperature condition of 80°C, according to the mass ratio of activated carbon to feed solution 1 is 1:100, add activated carbon to decolorize feed solution 1 for 35min, and then filter with plate frame and inorganic ceramic membrane successively to obtain filtrate 3. The filtrate 3 was cooled to 50° C. with a plate hea...

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Abstract

The invention discloses a new process for extracting lactic acid from lactic acid fermentation liquid. The process flow comprises the following steps: preparing the fermentation liquid, deproteinizing, removing bacteria, decolorizing with activated carbon, performing plate and frame filtration, filtering with an inorganic ceramic membrane, cooling by a plate type heat exchanger, filtering with an organic nano-filtration membrane, concentrating and performing primary crystallization, performing centrifugal drying, performing resolvation, performing acidolysis, passing through an ion exchange resin column and concentrating to obtain a finished product. The process flow performs membrane filtration twice, namely the filtration with the inorganic ceramic membrane and the filtration with the organic ceramic membrane, wherein the filtration with the inorganic ceramic membrane can remove small insoluble solid impurities, bacteria, colloidal substances, macromolecular sugars and other impurities in filtrate; the filtration with the organic nano-filtration membrane can remove low-molecular weight sugars and multivalent ions in the filtrate; and the purity of the finally prepared finished product lactic acid is as high as 95%-98%, the crystallization yield of the lactic acid is improved from original 70% to above 85%, and the production cost is greatly reduced.

Description

technical field [0001] The invention relates to a process for separating and purifying lactic acid, in particular to a process for separating and purifying lactic acid from lactic acid fermentation broth by using a membrane. Background technique [0002] Lactic acid, as an important organic acid, widely exists in human body, animal body and plant. Lactic acid, lactate and its derivatives are widely used in food, feed, chemical and pharmaceutical fields. In recent years, poly-L-lactic acid produced by the polymerization of L-lactic acid has been used as a new material to manufacture biodegradable plastics, green packaging materials and household films. The most promising new material in the new century has a very broad application prospect. [0003] The production methods of lactic acid include fermentation method, chemical synthesis method and enzymatic method. At present, the production of L-lactic acid with high optical purity is mainly based on microbial fermentation, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C59/08C07C51/487C07C51/47C07C51/42
Inventor 李悦明张希铭徐建春夏修峦王绍良
Owner QINGDAO KEHAI BIOLOGICAL
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