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Purely physical preparation method of vegetal and microbial polysaccharides

A microbial polysaccharide, pure physical technology, applied in the field of preparation of plant and microbial polysaccharides, can solve the problems of reduced protein content, increased polysaccharide extraction rate, shortened time consumption, etc., and achieves the effects of high purity, improved extraction rate and low cost

Inactive Publication Date: 2013-03-20
安徽本森堂生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a purely physical method for extracting polysaccharides from plants and microorganisms. The problem to be solved is to increase the extraction rate of polysaccharides, shorten the time-consuming, and reduce the protein content through the combination of high-pressure homogenization technology and ultrafiltration technology. The extraction process is pollution-free

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The fresh Polygonatum was dried at 40°C, and the dried Polygonatum was pulverized 3 times with a pulverizer, each time for 30 seconds. Pass the pulverized sealwort through a 60-mesh sieve, weigh 300 g of the sieved sealwort and put it into a container, add 20 times of water, and stir evenly.

[0031] Turn on the low-temperature circulating pump to maintain the internal working environment of the high-pressure homogenizer at 4~10°C, put the mixed liquid into the high-pressure homogenizer, first adjust the pressure to 5MPa, and circulate the material for 3 times, then adjust the pressure of the high-pressure homogenizer to 80MPa , and the number of homogenization is twice to obtain a homogeneous solution. Under the condition of 3000r / min, use a centrifuge to centrifuge the homogeneous solution for 20 minutes, collect the supernatant solution and place it in a container, and dry the residue for storage. The supernatant is graded and purified through ultrafiltration membra...

Embodiment 2

[0036] Preparation of Coomassie Brilliant Blue G-250 solution: Dissolve 100mg Coomassie Brilliant Blue G-250 in 95% ethanol, add 100ml 85% phosphoric acid, dissolve in a small amount of deionized water, then dilute to 1000ml constant volume with deionized water, filter through filter paper.

[0037] Preparation of standard protein solution: Accurately weigh 10.00 mg of bovine serum albumin, add water to dissolve it, set the volume to a 100 ml volumetric flask, and shake well to obtain a standard protein solution with a concentration of 100 μg / ml.

[0038] Drawing of standard curve: Accurately pipette 0.10ml, 0.20ml, 0.30ml, 0.40ml, 0.50ml, 0.60ml, 0.70ml, 0.80ml, 0.90ml of standard protein solution into 9 test tubes, add deionized water to make up to 1ml . Then add 5.0ml of Coomassie Brilliant Blue G-250 solution to each test tube, shake it well and let it stand for 2 minutes, measure the absorbance value at 595nm with a UV-visible spectrophotometer, use deionized water as a ...

Embodiment 3

[0041] Preparation of phenol solution: Use a pipette to accurately pipette 6.00ml of double-distilled phenol solution into a 100ml volumetric flask, and dilute with deionized water to obtain a 6% phenol solution.

[0042] Preparation of standard solution: Accurately weigh 10.00 mg of glucose that has been dried to constant weight, dissolve it in water, set the volume in a 100 ml volumetric flask, and shake well to obtain a standard solution of 100 μg / ml.

[0043] Drawing of standard curve: Accurately pipette 0.10ml, 0.20ml, 0.30ml, 0.40ml, 0.50ml, 0.60ml, 0.70ml, 0.80ml, 0.90ml of standard glucose solution into 9 test tubes, add deionized water to make up to 1ml , add 1ml of 6% phenol solution, mix well, then add 5ml of concentrated sulfuric acid, mix well, let it stand for 20min, use distilled water as blank, measure the absorbance at 490nm with a UV-visible spectrophotometer, and use A 490 The absorbance value is the abscissa, and the μg amount of glucose is the ordinate, in...

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Abstract

The invention discloses a purely physical preparation method of vegetal and microbial polysaccharides. The purely physical preparation method comprises the following steps of drying a sample, crushing the dried sample, sieving the sample powder by a sieve of 60 to 80 meshes, uniformly mixing the sample powder and water, pouring the mixture into a high pressure homogenizer, carrying out homogenization under certain pressure, carrying out centrifugation, collecting a supernatant, carrying out separation of the supernatant by an ultrafiltration membrane of which molecular weight cut-off is 2-5 times higher than molecular weight of vegetal or microbial active polysaccharides to obtain a penetrating fluid, carrying out separation of the supernatant by an ultrafiltration membrane of which molecular weight cut-off is 1 / 5-1 / 2 of molecular weight of the vegetal or microbial active polysaccharides to obtain a trapped fluid, concentrating the trapped fluid, and carrying out freeze drying to obtain an active polysaccharide sample. The purely physical preparation method has a high polysaccharide extraction ratio, short time consumption, protein-removal effects superior to those of the traditional sevage method, does not utilize any organic reagent in polysaccharide extraction, and has a low production cost and no pollution.

Description

technical field [0001] The invention relates to a preparation method of plant and microorganism polysaccharides, in particular to a purely physical preparation method for extracting plant and microorganism polysaccharides. Background technique [0002] Polysaccharides are an important class of macromolecular substances composed of more than 20 monosaccharides to tens of thousands of monosaccharides connected by glycosidic bonds. Biologically active polysaccharides are mainly divided into plant polysaccharides, microbial polysaccharides, and animal polysaccharides. As an important Macromolecules in organisms are closely related to the functions of organisms like nucleic acids and proteins. More and more studies have shown that polysaccharides have anti-tumor, anti-aging, antibacterial, anti-oxidation, hypoglycemic and immune-regulating functions. Therefore, polysaccharides have good development and application values. The polysaccharide extraction process has always been th...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 陈彦翟天龙段培鲁丁兢娜徐存吉郭文强王萌萌
Owner 安徽本森堂生物科技有限公司
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