Metarrhizium anisopliae granular preparation and preparation method thereof

A granule preparation, the technology of Metarhizium anisopliae, applied in botany equipment and methods, insecticides, acaricides, etc., can solve the problems of cost increase and unfavorable granule drying, reduce processing and manufacturing costs, and solve thermal damage and mechanical damage, reducing the effect of granulation heat generation

Active Publication Date: 2014-07-30
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The shortcoming of this method is: add a large amount of additives, cost obviously improves; Used binder, disintegrating agent, co-disintegrating agent, dispersant and coloring agent can have adverse effect on bacterium cell activity; It is not conducive to the drying of particles, which has increased energy consumption

Method used

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  • Metarrhizium anisopliae granular preparation and preparation method thereof
  • Metarrhizium anisopliae granular preparation and preparation method thereof
  • Metarrhizium anisopliae granular preparation and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The preparation of embodiment 1 metarhizium anisopliae granule preparation

[0022] Use highly efficient insecticidal fungus Metarhizium anisopliae strain MBJQH2-2, its preservation number is: CGMCC No.4275, which is recorded in Chinese patent publication CN102021122A, and the spore powder content is 2.0~5.0×10 10 / gram, the viable spore rate is greater than 80%, and the formula of the Metarhizium anisopliae granule preparation is as shown in the following table 1

[0023] Table 1 Metarhizium anisopliae granule formula

[0024]

[0025] After No. 1 to No. 5 pellets, the damage rates of Metarhizium anisopliae spores in the pellets were 96.82%, 95.08%, 100.00%, 100.00% and 28.84%, respectively. After granulation of No. 6~10, the damage rates of Metarhizium anisopliae spores in the granules were 25.83%, 24.76%, 23.39%, 23.02% and 21.94%, respectively. Compared with No. 5, the protection rates were 3.42%, 4.91%, 6.82%, respectively. %, 7.34% and 8.85%, the damage rate...

Embodiment 2-1

[0027] The impact of embodiment 2-1 carrier on the activity of Metarhizium anisopliae

[0028] (1) Effect of carrier leachate on spore germination

[0029] Weigh 1 g of each carrier, place them in sterilized test tubes containing glass beads and 0.1% Tween-80, vibrate evenly with a vortex oscillator, soak for 24 hours, and then centrifuge to get the supernatant. Take an appropriate amount of spore powder, put it in a sterilized test tube with glass beads, add the above-mentioned carrier supernatant, vortex and oscillate evenly to make a spore suspension, and adjust it to 107 spores / mL concentration, pipette 100 μL, and inoculate it on a sucrose agar plate. Three plates were inoculated for each vector as replicates. The spore suspension prepared with 0.1% Tween-80 was used as the control. After cultured at 25°C for 24 h, the germination rate of spores was examined under a microscope.

[0030] Analysis of variance showed that the 10 tested carriers had no significant effect...

Embodiment 2-2

[0034] The impact of embodiment 2-2 carrier on spore storage activity

[0035] Mix the spore powder and the sterilized carriers evenly according to the mass ratio of 5:95, divide them into 1.5mL centrifuge tubes, seal them and store them at 4°C, 15°C, and 25°C respectively. Take it out after 3 weeks and 4 weeks, and detect the amount of viable spores (number of colonies) contained in the unit mass. There are 5 plates for each carrier, and the pure spore powder is used as the control.

[0036] The effects of different carriers on the storage activity of spores were not the same, but the same carrier had the same effect on the storage activity of spores at different temperatures. Compared with the storage of pure spore powder, calcareous bentonite, kaolin, talcum powder, attapulgite and light carbonic acid can delay the inactivation of spores; perlite and diatomaceous earth can maintain spore viability within 2 weeks, but after 2 weeks the spore viability The decline was faster...

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Abstract

The invention relates to the field of biological pesticides, and specifically relates to a metarrhizium anisopliae granular preparation and a preparation method thereof. The metarrhizium anisopliae granular preparation comprises the following components in percentage by weight: 1-5% of metarrhizium anisopliae conidial powder, 90-95% of carriers, 0.01-0.45% of a promoting agent, 0.01-0.45% of a protective agent, and 1-5% of a lubricant. According to the technical scheme of the invention, the addition of aids is reduced, thereby significantly reducing the processing and manufacturing costs of granules. Under the premise of not adding an adhesive, a disintegrating agent and a disintegrating aid, through using the self physical and chemical properties of the carriers and the adjustment of water addition, the granulating property and the disintegration property of the carrier are solved well. Granules are relatively easy to dry, thereby reducing the energy consumption of drying. Through the adjustment of water addition, heat generated by granulating can be greatly reduced, and the damages on metarrhizium anisopliae spores caused by the granulating process can be reduced. Through adding small amount of the promoting agent, the germination of metarhizium anisopliae spores can be significantly promoted; and the damages on spores caused by the granulating process can be significantly reduced, and the problems of thermal damages and mechanical damages in the granulating process are solved better.

Description

technical field [0001] The application relates to the field of biopesticides, in particular to a metarhizium anisopliae granule preparation and a preparation method thereof. Background technique [0002] Metarhizium anisopliae is an insecticidal fungus distributed worldwide. It can parasitize more than 200 species of insects, mites and nematodes in 8 orders and 30 families. It is used as a biopesticide for the control of various agricultural and forestry pests. Therefore, in order to facilitate storage, field application and improve control effect, it is usually necessary to process Metarhizium anisopliae into various formulations. [0003] Granules are an ideal dosage form for controlling underground pests. They are not only convenient for spraying, but more importantly, provide a microenvironment conducive to the survival and colonization of Metarhizium anisopliae, thereby improving its environmental stress resistance and pest control effect. [0004] Chinese invention pa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/04A01N25/12A01P7/04A01P7/02A01P5/00
Inventor 农向群张英财张泽华曹广春王广君
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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