Preparation method of gamma-polyglutamic acid/Pulullan composite hydrogel

Abstract

The invention provides a preparation method of a gamma-polyglutamic acid / Pulullan composite hydrogel. The hydrogel contains gamma-polyglutamic acid, Pulullan and water. The preparation method comprises the following steps: dissolving 8-20% of gamma-polyglutamic acid in distilled water to obtain a first reaction solution; adding Pulullan according to different gamma-polyglutamic acid / Pulullan ratios to obtain a second reaction solution; and adding a crosslinking agent into the second reaction solution, stirring uniformly, regulating the pH value of the system, and reacting at 50 DEG C for 24 hours. The gamma-polyglutamic acid / Pulullan composite hydrogel prepared by the method has favorable adsorbability for brine. The hydrogel is applicable to a brine environment, and is especially applicable to an environment of soil, sweat, blood or the like.

Description

technical field

[0001] The invention relates to the field of composite hydrogels, in particular to a gamma-polyglutamic acid / pullulan composite hydrogel and a preparation method thereof. Background technique

[0002] γ-polyglutamic acid is an extracellular biopolymer amino acid polymer synthesized by microorganisms. It can form a super absorbent gel with a three-dimensional network structure through chemical crosslinking agents, γ-ray irradiation or other methods. Water absorption, hygroscopicity and water retention, etc., can absorb hundreds to thousands of times of its own mass of water, expand after absorbing water, and will not lose water after pressurization. It also has renewable and biodegradable properties. A water-retaining material that is friendly to the natural environment. It can be used in agriculture, food, cosmetics, hygiene products, biomedicine and other fields.

[0003] However, it is generally found in use that hydrogel can absorb thousands of times of ...

Examples

Embodiment 1

[0010] Example 1: 1.6g of γ-polyglutamic acid, add 18.4g of water, fully stir, then add 3.2g of pullulan, 1.28g of ethylene glycol diglycidyl ether, fully stir and dissolve to adjust the pH of the reaction solution to 4,50 ℃ under constant temperature reaction for 24h. The hydrogel was taken out and dissolved in physiological saline, the swelling was balanced, and the water absorption ratio was 53 times.

Embodiment 2

[0011] Example 2: 3.2g of γ-polyglutamic acid, add 16.8g of water, fully stir, then add 1.6g of pullulan, 1.28g of ethylene glycol diglycidyl ether, fully stir and dissolve to adjust the pH of the reaction solution to 6,50 ℃ under constant temperature reaction for 24h. Take out the hydrogel and dissolve it in distilled water, the swelling is balanced, and the water absorption rate is 260 times.

Embodiment 3

[0012] Example 3: 3.2g of γ-polyglutamic acid, add 16.8g of water, fully stir, then add 6.4g of pullulan, 1.92g of ethylene glycol diglycidyl ether, fully stir and dissolve to adjust the pH of the reaction solution to 4,50 ℃ under constant temperature reaction for 24h. The hydrogel was taken out and dissolved in physiological saline, the swelling was balanced, and the water absorption rate was 37 times.

technical field

[0001] The invention relates to the field of composite hydrogels, in particular to a gamma-polyglutamic acid / pullulan composite hydrogel and a preparation method thereof. Background technique

[0002] γ-polyglutamic acid is an extracellular biopolymer amino acid polymer synthesized by microorganisms. It can form a super absorbent gel with a three-dimensional network structure through chemical crosslinking agents, γ-ray irradiation or other methods. Water absorption, hygroscopicity and water retention, etc., can absorb hundreds to thousands of times of its own mass of water, expand after absorbing water, and will not lose water after pressurization. It also has renewable and biodegradable properties. A water-retaining material that is friendly to the natural environment. It can be used in agriculture, food, cosmetics, hygiene products, biomedicine and other fields.

[0003] However, it is generally found in use that hydrogel can absorb thousands of times of ...

Examples

Embodiment 1

[0010] Example 1: 1.6g of γ-polyglutamic acid, add 18.4g of water, fully stir, then add 3.2g of pullulan, 1.28g of ethylene glycol diglycidyl ether, fully stir and dissolve to adjust the pH of the reaction solution to 4,50 ℃ under constant temperature reaction for 24h. The hydrogel was taken out and dissolved in physiological saline, the swelling was balanced, and the water absorption ratio was 53 times.

Embodiment 2

[0011] Example 2: 3.2g of γ-polyglutamic acid, add 16.8g of water, fully stir, then add 1.6g of pullulan, 1.28g of ethylene glycol diglycidyl ether, fully stir and dissolve to adjust the pH of the reaction solution to 6,50 ℃ under constant temperature reaction for 24h. Take out the hydrogel and dissolve it in distilled water, the swelling is balanced, and the water absorption rate is 260 times.

Embodiment 3

[0012] Example 3: 3.2g of γ-polyglutamic acid, add 16.8g of water, fully stir, then add 6.4g of pullulan, 1.92g of ethylene glycol diglycidyl ether, fully stir and dissolve to adjust the pH of the reaction solution to 4,50 ℃ under constant temperature reaction for 24h. The hydrogel was taken out and dissolved in physiological saline, the swelling was balanced, and the water absorption rate was 37 times.