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Protein related to plant epidermal hair growth, coding gene of protein related to plant epidermal hair growth and application

A technology encoding genes and proteins, applied in the direction of plant gene improvement, application, plant peptides, etc., to achieve the effect of inhibiting the formation of epidermal hair

Inactive Publication Date: 2014-03-19
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there has never been a report on the existence of TRY gene in multicellular epidermal plants such as cucumber fruit thorns

Method used

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  • Protein related to plant epidermal hair growth, coding gene of protein related to plant epidermal hair growth and application
  • Protein related to plant epidermal hair growth, coding gene of protein related to plant epidermal hair growth and application
  • Protein related to plant epidermal hair growth, coding gene of protein related to plant epidermal hair growth and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1, the cloning of the gene CsTRY related to the development of plant epidermis

[0037] 1. Materials

[0038]Cucumber Jinyan No. 4 (purchased from the Cucumber Research Institute of Tianjin Academy of Agricultural Sciences) was used as the test material. This variety has obvious hairy skin and dense thorn tumors. Cucumber seeds were sown in a solar greenhouse for ordinary cultivation and management. When the plants began to produce melons, the fruits on the day of flowering were taken and frozen in liquid nitrogen, and stored in a -80°C refrigerator for later use.

[0039] 2. Total RNA extraction and cDNA synthesis

[0040] 1. Extraction of total RNA

[0041] The total RNA of cucumber fruit was extracted with a column plant RNAout kit (purchased from Beijing Tianenze Gene Technology Co., Ltd., catalog number 71203), and the residual DNA in the RNA sample was removed with DNase. Proceed as follows:

[0042] (1) Add about 200 mg of cucumber fruit into a mor...

Embodiment 2

[0075] Embodiment 2, the acquisition of transgenic plants and its detection

[0076] 1. Construction of a recombinant expression vector containing the target gene CsTRY

[0077] 1. Construction of CsTRY overexpression vector

[0078] The positive bacterial plasmid CsTRY-PBSK with correct sequencing and the overexpression vector ms1300 (the public can obtain from China Agricultural University, the non-patent literature that has recorded this material is: Liao Hui. Phd cold resistance research and screening analysis of ABA sensitive mutation. Master Dissertation.p23) Carry out sacI and HindIII double enzyme digestion at the same time, and digest overnight at 37°C. After digestion, the 249bp target fragment and the large fragment of the overexpression vector were recovered, ligated with T4DNA Ligase, and ligated overnight at 16°C to obtain the target plasmid. The target plasmid was transformed into Escherichia coli, resistance screening, positive clones were picked, the positiv...

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Abstract

The invention discloses a protein related to plant epidermal hair growth, a coding gene of the protein related to plant epidermal hair growth and an application. The invention provides the protein related to plant epidermal hair growth. The protein related to plant epidermal hair growth provided by the invention is the protein shown in 1 or 2: 1, the protein consists of an amino acid sequence which is shown in a sequence 1 in a sequence table; and 2, the protein is formed by substituting and / or deleting and / or adding one or a plurality of amino acid residues in an amino acid residue sequence in the sequence 1 in the sequence table, is related to plant epidermal hair growth and is derived from the protein in 1. Experiments prove that the overexpression of heterogenous CsTRY can cause the sharp reduction of arabidopsis thaliana leaf epidermal hairs, and the expression quantities of genes of GLABRA2 (GL2), TRICHOMELESS1 (TCL1) and the like in arabidopsis thaliana can be obviously reduced; experiments show that a CsTRY gene can inhibit the epidermal hairs from forming as an arabidopsis thaliana TRY gene; and the protein plays an important role in improving cucumber quality.

Description

technical field [0001] The invention relates to a protein related to the development of plant epidermis and its coding gene and application in the field of biotechnology. Background technique [0002] Cucumber (cucumis sativus L.) is an annual herbaceous plant belonging to the genus Cucumis in the family Cucurbitaceae. As one of the ten most important vegetable crops in the world, cucumber is also one of the main vegetable crops in my country, accounting for about 10% of the national vegetable area. Most of the quality genes that have been identified so far are sensory quality genes, such as the presence or absence of tumors, the color of fruit thorns, the number of fruit thorns, the color of fruit peel, and the structure of fruit peel. The fruit tumors and fruit thorns of the North China type cucumbers in my country are large and many, the fruit tumors and fruit spines of the South China type cucumbers are relatively small, the European greenhouse type cucumbers have no fr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/63C12N5/10C12N1/21C12N15/11C12N15/82C12N15/84A01H5/00
Inventor 任华中谭峥郭芳刘兴旺刘斌
Owner CHINA AGRI UNIV
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