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Method for producing camellia oil cake feed through mixed strain fermentation

A technology of tea seed meal and fermented raw materials, applied in animal feed, animal feed, applications, etc., can solve the problems of high cellulose content of tea seed meal and poor palatability of feed, so as to reduce hemolysis and facilitate growth and reproduction , the effect of improving palatability

Inactive Publication Date: 2014-06-11
SHANGHAI JIAOTONG UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The cellulose content of tea seed meal is high, and the palatability of feed is not good

Method used

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  • Method for producing camellia oil cake feed through mixed strain fermentation
  • Method for producing camellia oil cake feed through mixed strain fermentation

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Embodiment 1

[0021] 1. Preparation medium:

[0022] MRS medium (Lactobacillus bread): peptone 10.0g, beef extract 10.0g, yeast extract 5.0g, cellobiose 20.0g, Tween-801ml, triammonium citrate 2.0g, K 2 HPO 4 2.0g, MgSO 4 ·7H 2 00.2g, MnSO 4 2H 2 O0.05g, distilled water 1000ml, 121 ℃, high-pressure sterilization 15min, solid culture medium is to add agar 18.0g in liquid medium, then 121 ℃ high-pressure sterilization 15min.

[0023] LB medium (Bacillus natto): tryptone 10g, yeast extract 5g, NaCl 10g, distilled water 1000ml. 121°C, autoclave for 15 minutes, the solid medium is to add 18.0 g of agar to the liquid medium, and then sterilize under high pressure at 121°C for 15 minutes.

[0024] The ingredients of seed medium A are: peptone 10.0g, beef extract 10.0g, yeast extract 5.0g, cellobiose 20.0g, Tween-801.0ml, triammonium citrate 2.0g, K 2 HPO 4 2.0g, MgSO 4 ·7H 2 00.2g, MnSO 4 2H 2 O0.05g, distilled water 1000ml.

[0025] The components of seed medium B are: tryptone 10g, ...

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Abstract

The invention relates to a method for producing camellia oil cake feed through mixed strain fermentation. Bread lactobacillus and bacillus natto are inoculated into camellia oil cake and are subjected to aerobic fermentation and anaerobic fermentation to produce low-toxicity feed. The method provided by the invention overcomes the shortcomings of camellia oil cake in feed application, degrades tea saponin and reduces the hemolysis thereof, degrades cellulose in camellia oil cake, produces flavor substance through lactobacillus fementation and improves the palatability of the feed; and moreover, since bacillus natto is a subspecies of bacillus subtilis, the bacillus natto after heating and drying still exists in the fermented camellia oil cake in a form of spores and thus can realize the function of probiotics.

Description

Technical field: [0001] The invention relates to a method for fermenting and producing tea seed meal, in particular to a method for producing tea seed meal feed by mixing and fermenting Lactobacillus crustorum and Bacillus natto. Background technique: [0002] The toxicity of saponins is mainly manifested as hemolysis. It is generally believed that the hemolysis is that its aglycone can combine with cholesterol on the red blood cell wall to form a water-insoluble complex precipitation, which destroys the osmotic pressure of the cell, causes the red blood cell to disintegrate, and then leads to hemolysis. . The hemolytic activity of saponins is related to saponin, and the activity is related to the size of sugar molecules, the connection between sugar molecules, the substituents on sugar molecules, etc., and also related to the concentration of saponins. [0003] The structure of tea saponin is attached figure 1 As shown, it belongs to triterpenoid saponins, which are compo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K1/14
Inventor 张建华尹丽茸倪银芸左琦李双双林立
Owner SHANGHAI JIAOTONG UNIV
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