Norwogonin and application thereof to preparation of medicines for treating acinetobacter baumannii infection

A technology of wogonin and Acinetobacter baumannii, which is applied in the field of medicine and achieves the effect of high safety

Active Publication Date: 2013-04-17
程新明
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, no wogonin has been reported for t

Method used

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  • Norwogonin and application thereof to preparation of medicines for treating acinetobacter baumannii infection
  • Norwogonin and application thereof to preparation of medicines for treating acinetobacter baumannii infection
  • Norwogonin and application thereof to preparation of medicines for treating acinetobacter baumannii infection

Examples

Experimental program
Comparison scheme
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Example Embodiment

[0027] Example 1 Determination of the minimum inhibitory concentration MIC of wogonin against multidrug-resistant Acinetobacter baumannii

[0028] 1.1 Determination of drug resistance of Acinetobacter baumannii

[0029] It was identified by VITEK-AMS analyzer of French bio-Mérieux Company, combined with routine drug susceptibility tests, and confirmed that the bacteria are resistant to the following antibiotics: cephalosporins against pseudomonas, aminoglycoside antibiotics, β-lactams / β-lactams Enzyme inhibitor compound preparations, fluoroquinolone antibiotics (including norfloxacin, pefloxacin, ciprofloxacin), tetracycline, tigecycline, etc. are resistant to imipenem and polymyxa Sensitive.

[0030] Experimental results: the Acinetobacter baumannii of the present invention is isolated and obtained from the sputum of patients, and has strong reference; drug resistance analysis shows that the Acinetobacter baumannii is resistant to a variety of antibiotic drugs, and it is a m...

Example Embodiment

[0040] Example 2 The lowest bactericidal concentration MBC experiment of wogonin against multi-drug resistant Acinetobacter baumannii

[0041] Take 1.5ml of wogonin at different dilution concentrations in step 1.3 and add them to different test tubes, and then add 15ul of 1X10 to each test tube. 7 CFU / ml bacterial liquid, mix well, incubate at 37 ° C for 18 hours, take 0.1 ml of each concentration of drug-bacterial liquid and inoculate on MH medium agar plate, and then cultivate at 37 ° C for 24 hours, according to the colony count standard: The number of colonies was less than 5.

[0042] Experimental results: The minimum drug concentration corresponding to aseptic growth in the plate (the number of colonies is less than 5) is recorded as the minimum bactericidal concentration MBC of the drug; The killing rate of norwogonin against multidrug-resistant Acinetobacter baumannii was 99.9%.

Example Embodiment

[0043] Example 3 Determination of the combined drug susceptibility of norwogonin and imipenem to multidrug-resistant Acinetobacter baumannii by checkerboard method

[0044] Dilute imipenem and wogonin to 8 concentrations in sterilized MH medium respectively, the highest drug concentration is the respective 2xMIC, the minimum drug concentration is the respective 1 / 64xMIC, and the concentration of wogonin is 8 dilution concentrations are 256ug / ml, 128ug / ml, 64ug / ml, 32ug / ml, 16ug / ml, 8ug / ml, 4ug / ml, 2ug / ml. The 8 dilution concentrations of imipenem are 512ug / ml, 256ug / ml, 128ug / ml, 64ug / ml, 32ug / ml, 16ug / ml, 8ug / ml, 4ug / ml. The prepared wogonin and imipenem with different concentrations were designed according to the checkerboard method, and added to the 96-well plate in two-by-two combinations. Take 50ul of each drug, and the two drugs are 100ul in total; Drug control, that is, 50ul of single drug per well plus 50ul of MH medium, the total volume is 100ul; then add 1.5x10 to e...

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Abstract

The invention relates to an application of norwogonin to preparation of medicines for treating acinetobacter baumannii infection and complications caused by the acinetobacter baumannii infection. Acinetobacter baumannii is resistant to a variety of antibiotics and the norwogonin can inhibit growth of the acinetobacter baumannii with multidrug resistance; an in-vitro drug susceptibility test proves that during independent use of the norwogonin, the minimum inhibitory concentration of the norwogonin is half of the minimum inhibitory concentration of the conventional therapeutic medicine imipenem, which means that the inhibitory effect of the norwogonin is better; combination of the norwogonin and the imipenem can achieve a synergistic effect on the growth of the acinetobacter baumannii with the multidrug resistance; and an acute toxicity test on a mouse proves that the using safety of the norwogonin is high, which means that the norwogonin has a good medicinal prospect.

Description

technical field [0001] The invention relates to a flavonoid compound, in particular to norwogonin and its application in the preparation of medicines for treating Acinetobacter baumannii infection, belonging to the technical field of medicine. Background technique [0002] Scutellaria baicalensis is the dried root of Scutellaria baicalensis Georgi (Lamiaceae), a perennial herb of the Lamiaceae family. Oxidation, scavenging free radicals, anti-inflammation, anti-tumor, blocking calcium ion channels, inhibiting aldose reductase, anti-virus, anti-allergic, etc., and has protective effects on immune, cardiovascular, digestive, nervous and other systems. In recent years, researchers have conducted in-depth research on the components and pharmacology of Scutellaria baicalensis, among which the chemical components mainly focus on baicalein, cuogono side, wogonin and other components. Wogonin can inhibit the biofilm formation of Pseudomonas aeruginosa and Candida albicans, reduce t...

Claims

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Application Information

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IPC IPC(8): A61K31/352A61P31/04
Inventor 程新明徐泽松
Owner 程新明
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