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Norwogonin and application thereof to preparation of medicines for treating acinetobacter baumannii infection

A technology of wogonin and Acinetobacter baumannii, which is applied in the field of medicine and achieves the effect of high safety

Active Publication Date: 2013-04-17
程新明
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, no wogonin has been reported for the treatment of Acinetobacter baumannii infection.

Method used

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  • Norwogonin and application thereof to preparation of medicines for treating acinetobacter baumannii infection
  • Norwogonin and application thereof to preparation of medicines for treating acinetobacter baumannii infection
  • Norwogonin and application thereof to preparation of medicines for treating acinetobacter baumannii infection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 The minimum inhibitory concentration MIC determination experiment of norwogonin on multi-drug resistant Acinetobacter baumannii

[0028] 1.1 Determination of drug resistance of Acinetobacter baumannii

[0029] Identified by the VITEK-AMS analyzer of BioMérieux, France, and combined with routine drug sensitivity tests, it was confirmed that the bacteria are resistant to the following antibiotics: cephalosporins against pseudomonas, aminoglycoside antibiotics, β-lactams / β-lactams Enzyme inhibitor compound preparations, fluoroquinolone antibiotics (including norfloxacin, pefloxacin, ciprofloxacin), tetracycline, tigecycline, etc. are resistant to imipenem and polymyxa Susceptible.

[0030] Experimental results: the Acinetobacter baumannii of the present invention is isolated from the patient's sputum, and has strong reference; the analysis of drug resistance shows that this Acinetobacter baumannii is resistant to multiple antibiotics, and is a multi-resistant dr...

Embodiment 2

[0040] Example 2 The minimum bactericidal concentration MBC experiment of norwogonin to multi-drug resistant Acinetobacter baumannii

[0041] Take 1.5ml of norwogonin with different dilution concentrations in step 1.3 and add them to different test tubes respectively, and then add 15ul1X10 to each test tube. 7 CFU / ml bacterial solution, mix well, incubate at 37°C for 18 hours, take 0.1ml of drug-bacterial solution of each concentration and inoculate it on the MH medium agar plate, then incubate at 37°C for 24 hours, according to the colony counting standard The number of colonies is less than 5.

[0042] Experimental results: The minimum drug concentration corresponding to the aseptic growth in the plate (the number of colonies is less than 5) is recorded as the minimum bactericidal concentration MBC of the drug; the measured MBC value of norwogonin is 256ug / ml, that is, the concentration of 256ug / ml The killing rate of norwogonin against multidrug-resistant Acinetobacter bau...

Embodiment 3

[0043] Example Three Chessboard Method Determination of combined drug susceptibility of norwogonin and imipenem to multi-drug resistant Acinetobacter baumannii

[0044] Dilute imipenem and norwogonin to 8 concentrations in sterilized MH medium, the highest drug concentration is 2xMIC, the lowest drug concentration is 1 / 64xMIC, and the concentration of norwogonin The 8 dilution concentrations are 256ug / ml, 128ug / ml, 64ug / ml, 32ug / ml, 16ug / ml, 8ug / ml, 4ug / ml, 2ug / ml. The 8 dilution concentrations of imipenem are 512ug / ml, 256ug / ml, 128ug / ml, 64ug / ml, 32ug / ml, 16ug / ml, 8ug / ml, 4ug / ml. The prepared norwogonin and imipenem of different concentrations were designed according to the checkerboard method, and were added to the 96-well plate in pairs, with 50ul for each drug, and a total of 100ul for the two drugs; Drug control, that is, 50ul of single drug per well plus 50ul of MH medium, with a total volume of 100ul; then add 1.5x10 5 CFU / ml bacterial suspension 100ul. In addition,...

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Abstract

The invention relates to an application of norwogonin to preparation of medicines for treating acinetobacter baumannii infection and complications caused by the acinetobacter baumannii infection. Acinetobacter baumannii is resistant to a variety of antibiotics and the norwogonin can inhibit growth of the acinetobacter baumannii with multidrug resistance; an in-vitro drug susceptibility test proves that during independent use of the norwogonin, the minimum inhibitory concentration of the norwogonin is half of the minimum inhibitory concentration of the conventional therapeutic medicine imipenem, which means that the inhibitory effect of the norwogonin is better; combination of the norwogonin and the imipenem can achieve a synergistic effect on the growth of the acinetobacter baumannii with the multidrug resistance; and an acute toxicity test on a mouse proves that the using safety of the norwogonin is high, which means that the norwogonin has a good medicinal prospect.

Description

technical field [0001] The invention relates to a flavonoid compound, in particular to norwogonin and its application in the preparation of medicines for treating Acinetobacter baumannii infection, belonging to the technical field of medicine. Background technique [0002] Scutellaria baicalensis is the dried root of Scutellaria baicalensis Georgi (Lamiaceae), a perennial herb of the Lamiaceae family. Oxidation, scavenging free radicals, anti-inflammation, anti-tumor, blocking calcium ion channels, inhibiting aldose reductase, anti-virus, anti-allergic, etc., and has protective effects on immune, cardiovascular, digestive, nervous and other systems. In recent years, researchers have conducted in-depth research on the components and pharmacology of Scutellaria baicalensis, among which the chemical components mainly focus on baicalein, cuogono side, wogonin and other components. Wogonin can inhibit the biofilm formation of Pseudomonas aeruginosa and Candida albicans, reduce t...

Claims

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Application Information

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IPC IPC(8): A61K31/352A61P31/04
Inventor 程新明徐泽松
Owner 程新明
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