Bacterial cellulose/collagen composite material having biocompatibility and preparation method thereof

A technology of bacterial cellulose and biocompatibility, applied in the field of biomedicine, can solve the problems that the biological activity cannot meet the wide application in the medical field, and achieve the effect of retaining the advantages of structure and performance, easy access to raw materials, and uniform synthesis

Active Publication Date: 2014-08-27
CHINA TIANCHEN ENG +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the deepening of research, it can be found that BC is a polysaccharide substance, which is a kind of ultrafine and ultra-pure cellulose, which has the same molecular structure unit as the natural cellulose produced by plants or seaweed in nature, and has certain biological activities. , but compared with materials with better biological activity (such as chitosan, gelatin, silk protein, etc.), its biological activity cannot meet the wide application in the medical field

Method used

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  • Bacterial cellulose/collagen composite material having biocompatibility and preparation method thereof
  • Bacterial cellulose/collagen composite material having biocompatibility and preparation method thereof
  • Bacterial cellulose/collagen composite material having biocompatibility and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0023] The bacterial cellulose secreting strain of the present invention is Acetobacter xylinum (Ax). Ax is Gram-negative, 0.6-0.8 μm wide, 1.0-4.0 μm long, light brown, round, exists in single, paired or chain form, opaque, with protruding colonies, rough surface, and is aerobic. It has been used as a model microorganism for basic and applied research on bacterial cellulose.

[0024] 1. Preparation of bacterial cellulose

[0025] Glucose 5.0% (w / v) (or mannitol 10% (w / v)), soybean peptone 0.9% (w / v), Na2HPO4·12H2O0.8% (w / v) and citric acid 0.5% (w / v) / v) Add to the beaker in sequence, adjust the pH value to the range of 5.5-7.0 with acetic acid, take out after high temperature sterilization at 115° C. for half an hour, and use it as a growth medium for Acetobacter xylinum.

[0026] Acetobacter xylinum strains were inoculated into the above-mentioned liquid medium, shaken sufficiently to make the bacterial liquid uniform, and cultured at a constant temperature of 28° C. for ...

Embodiment 2

[0031] 1. Preparation of bacterial cellulose

[0032] Glucose 5.0% (w / v) (or mannitol 10% (w / v)), soybean peptone 0.9% (w / v), Na2HPO4·12H2O0.8% (w / v) and citric acid 0.5% (w / v) / v) Add to the beaker in sequence, adjust the pH value to the range of 5.5-7.0 with acetic acid, take out after high temperature sterilization at 115° C. for half an hour, and use it as a growth medium for Acetobacter xylinum.

[0033] Acetobacter xylinum strains were inoculated into the above-mentioned liquid medium, shaken sufficiently to make the bacterial liquid uniform, and cultured at a constant temperature of 28° C. for 3 days to obtain bacterial cellulose. Alternately wash and boil with 1% sodium hydroxide solution and deionized water until milky white, and finally wash with deionized water until neutral. The bacterial cellulose obtained at this time is wet state bacterial cellulose.

[0034] 2. Preparation of bacterial cellulose / collagen composites

[0035] 100 ml of 1 g / L collagen (sigma, US...

Embodiment 3

[0037] 1. Preparation of bacterial cellulose

[0038] Glucose 5.0% (w / v) (or mannitol 10% (w / v)), soybean peptone 0.9% (w / v), Na2HPO4·12H2O0.8% (w / v) and citric acid 0.5% (w / v) / v) Add to the beaker in sequence, adjust the pH value to the range of 5.5-7.0 with acetic acid, take out after high temperature sterilization at 115° C. for half an hour, and use it as a growth medium for Acetobacter xylinum.

[0039]Acetobacter xylinum strains were inoculated into the above-mentioned liquid medium, shaken sufficiently to make the bacterial liquid uniform, and cultured at a constant temperature of 28° C. for 3 days to obtain bacterial cellulose. Alternately wash and boil with 1% sodium hydroxide solution and deionized water until milky white, and finally wash with deionized water until neutral. The bacterial cellulose obtained at this time is wet state bacterial cellulose.

[0040] 2. Preparation of bacterial cellulose / collagen composites

[0041] 100 ml of 3.5 g / L collagen (sigma, U...

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Abstract

The invention provides a bacterial cellulose / collagen composite material having biocompatibility and a preparation method thereof. The invention is characterized in that the composite material is prepared by taking bacterial cellulose, collagen and genipin as raw materials, wherein the mass ratio of the bacterial cellulose, the collagen and the genipin is (5-200):(0.1-5):(0.1-5). The invention has the characteristics of high synthesis efficiency, favorable synthesis uniformity, maintained structure and performance advantages of original BC and the like. In addition, the in vitro culture with fibroblasts discovers that the composite material has better biocompatibility.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a biocompatible bacterial cellulose / collagen composite material and a preparation method thereof. Background technique [0002] In daily life, diseases and accidents often lead to pathological changes and damages to organs or tissues of the human body. When lesions and injuries cannot be repaired by the body's own system, it is necessary to rely on external medical means to achieve. In the process of exploring and developing medical engineering, tissue engineering research has become a hot spot in scientific research and clinical application. Tissue engineering includes three major elements: artificial extracellular matrix-scaffold, cells, and information factors. Among them, the research on tissue engineering scaffold materials is the key factor that determines the success or failure of tissue engineering. At present, many polymer tissue engineering scaffold materials such as polyla...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/24A61L27/20A61L27/60
Inventor 徐俊青李丽叶建国邢胜男李秀萍
Owner CHINA TIANCHEN ENG
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