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Method for distinguishing root-bark medicinal materials of celastrus angulatus

A technology of bitter bark root bark and medicinal materials, which is applied in the field of identification of natural medicinal plants, and can solve problems such as not being practically applied

Active Publication Date: 2014-06-11
河南省科高植物天然产物开发工程技术有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

About bitter bark vine medicinal materials 1 H NMR fingerprint spectrum [Qin Hailin et al. 1 Analysis of H NMR fingerprints. Acta Pharmaceutica Sinica, 2001, 36 (6): 462-466] and infrared fingerprints [Liu Huixia, et al. Infrared spectral characteristics of Phyllostachys chinensis and its application in identification. Journal of Northwest Botany 1998, 305 -310] has been reported, only qualitative analysis, no practical application
And, there is no report on IGD carbon NMR coupled fingerprint

Method used

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  • Method for distinguishing root-bark medicinal materials of celastrus angulatus
  • Method for distinguishing root-bark medicinal materials of celastrus angulatus
  • Method for distinguishing root-bark medicinal materials of celastrus angulatus

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0078] ④ Preparation of the test solution

[0079] Accurately weigh 200mg of the characteristic extract of the root bark of the bitter bark into a 100mL volumetric flask, add an appropriate amount of methanol to dissolve, dilute to the mark after ultrasonic oscillation, and shake well to obtain the test solution of the characteristic extract of the root bark of the bitter bark.

[0080] ⑤Measurement of precision

[0081] The test solution was injected three times, the relative standard deviation of the peak area was RSD=0.26%, and the relative standard deviation of the retention time was RSD=0.19%.

[0082] ⑥ Determination of the test product

[0083] Draw each test product solution, inject sample, measure its peak area, and obtain the content of picrocetin B.

[0084] ⑦Determination of recovery rate

[0085] Using the standard addition method, 100 μg / mL was added to the test sample 1, and the average recovery rate was between 87.0-102.9%.

[0086] 2) Calculation of the ab...

Embodiment 1

[0233] Example 1: IGD C-NMR Spectrum-Coupled Fingerprint of the Root Bark of Phyllostachys chinensis in Haozhou, Anhui

[0234] (1) Preparation of characteristic extracts

[0235] Select the root bark of the bitter vine in Haozhou, Anhui, and prepare the characteristic extract of the root bark of the bitter vine according to the following method: Weigh 100 g of the root and bark of the bitter vine, crush it (through a 24 mesh sieve), and use 6 times the amount each time Volume (600mL), mass concentration of 95% ethanol reflux extraction 3 times, 2 hours each time, the filtrates were combined and then concentrated under reduced pressure, and the solvent was recovered to powder, and the characteristic extract of the root bark of Phyllostachys chinensis was obtained.

[0236] (2) IGD carbon NMR spectrum test of medicinal material characteristic extracts

[0237] Take 1 g of the characteristic extract of the root bark of Phyllostachys chinensis, add six times the amount (6 mL) of...

Embodiment 2

[0248] Example 2: IGD Carbon-NMR Spectrum-Coupled Fingerprints of the Root Bark of Phyllostachys chinensis in Baoji, Shaanxi

[0249] (1) Preparation of characteristic extracts

[0250] The difference from Example 1 is that the root bark of the vine root bark of Baoji, Shaanxi is selected, and the others are identical.

[0251] (2) Feature extraction IGD carbon NMR spectrum test

[0252] Same as Example 1.

[0253] (3) IGD carbon NMR fingerprint analysis

[0254] 1) identification

[0255] The characteristic signals of 4-OH-β-dihydroagarfuran sesquiterpene polyol esters are clearly shown in the IGD carbon NMR fingerprints of the characteristic extracts from the roots and bark of Pittonia fraternica produced in Baoji, Shaanxi. According to the chemical shift of its parent nucleus signal and its C-15 characteristic peak signal, 15 4-OH-β-dihydroagarfuran sesquiterpene polyol ester compounds picrocetin A, B, C, E, F were confirmed , G, H, J, P, U, X, celangulatin C, paretin ...

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Abstract

The invention provides a method for distinguishing root-bark medicinal materials of celastrus angulatus. The method comprises the steps of: extracting the root-bark medicinal materials of the celastrus angulatus to obtain feature extract of the root-bark medicinal materials of the celastrus angulatus containing active ingredients; carrying out IGD nuclear magnetic resonance carbon spectrum fingerprint detection on the feature extract, and obtaining peak intensities of a plurality of characteristic peaks of the active ingredients in the feature extract according to a finger-print; determining the peak intensities of the characteristic peaks of a standard reference substance corresponding to each active ingredient in a same manner; determining absolute content of the standard reference substance in the root-bark medicinal materials of the celastrus angulatus by a quantitative analysis method; and calculating the content of each active ingredient and the contents of the active ingredients in the root-bark medicinal materials of the celastrus angulatus by the ratio of the peak intensities of the characteristic peaks and the absolute content. According to the invention, the situation that the root and the bark of the celastrus angulatus contain 4-OH-beta-dihydroagarofuran sesquiterpene polyol ester compound, and the ratio thereof are reflected by an IGD nuclear magnetic resonance carbon spectrum coupling fingerprint technology, and identification of the variety and the quality of the root-bark medicinal materials of the celastrus angulatus is finished.

Description

technical field [0001] The invention belongs to the field of identification of natural medicinal plants, and in particular relates to a method for identification of root bark medicinal materials of phalaetum. Background technique [0002] Celastrus angulatus (Celastrus angulatus), also known as horse heartbroken, bitter bark, tiger hemp, etc., is a perennial vine-like shrub of Celastraceae (Celastraceae), produced in Hebei, Shandong, Henan, Shaanxi, Hubei, Gansu, Jiangsu, Anhui and other provinces. Its roots, stems, leaves, fruits and seeds are natural insecticides and important traditional Chinese medicine resources. It has a long history of folks using its root bark, stem bark and leaves to control insect pests in vegetables and various crops[①China Chinese Academy of Sciences Flora of China Editorial Committee. Flora of China. Science Press, 1999, 45(3): 102. ② Ke Zhiguo, et al. The research and advances on the plant-based insect antifeedant Celastrus angulatus[J]. Wuhan...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N24/08G01N30/02
Inventor 张海艳赵天增董建军郭唯范毅张义东
Owner 河南省科高植物天然产物开发工程技术有限公司
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