Preparation method for biosensor based on graphene/carbon nano-tube

A technology of biosensors and carbon nanotubes, applied in instruments, scientific instruments, measuring devices, etc., can solve the problems of carcinogenicity, environmental pollution, and mutagenicity in animals, improve the load rate, make the preparation method simple and easy, and improve electron transfer speed effect

Inactive Publication Date: 2013-04-17
TONGJI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The number of pesticide poisoning in China is also increasing. According to the data, 75.14% of the pesticide poisoning is caused by organophosphorus pesticides. produce serious pollution

Method used

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  • Preparation method for biosensor based on graphene/carbon nano-tube
  • Preparation method for biosensor based on graphene/carbon nano-tube
  • Preparation method for biosensor based on graphene/carbon nano-tube

Examples

Experimental program
Comparison scheme
Effect test

Embodiment l

[0027] 1) Preparation of functionalized graphene GO-PEG: 200 mg of graphite oxide GO was added to 10 ml of DMF, and ultrasonically treated at 70 kHz for 3 hours to obtain a stable GO / DMF dispersion. 40ml SOCl 2 Add it to the dispersion liquid, reflux for 48 hours at a temperature of 80°C, and distill off excess SOCl 2 . Then 2g of PEG4000 was added to the reactant, and the reaction was refluxed at 120°C for 96 hours, washed with ethanol and water until neutral, and dried in vacuum at 60°C to obtain functionalized graphene GO-PEG.

[0028] (2) Mix 0.5g of carbon nanotubes and 100mL of strong oxidizing acid, treat it under 120kHz ultrasonic wave for 5 hours, then heat to 120°C, stir and reflux for 72 hours, dilute and wash with deionized water and filter with microporous membrane , until the filtrate is neutral, and vacuum-dried at 60° C. for 48 hours to obtain carboxylated carbon nanotubes.

[0029] (3) Preparation of biosensors:

[0030] a. Pretreatment of glassy carbon e...

Embodiment 2

[0034] 1) Preparation of functionalized graphene GO-PEG: 200 mg of graphite oxide GO was added to 10 ml of DMF, and ultrasonically treated at 70 kHz for 3 hours to obtain a stable GO / DMF dispersion. 40ml SOCl 2 Add it to the dispersion liquid, reflux for 48 hours at a temperature of 80°C, and distill off excess SOCl 2 . Then 2g of PEG600 was added to the reactant, and the reaction was refluxed at 120°C for 72 hours, washed with ethanol and water until neutral, and dried in vacuum at 60°C to obtain functionalized graphene GO-PEG.

[0035] (2) Mix 0.5g of carbon nanotubes and 100mL of strong oxidizing acid, treat it under 120kHz ultrasonic wave for 5 hours, then heat to 120°C, stir and reflux for 72 hours, dilute and wash with deionized water and filter with microporous membrane , until the filtrate is neutral, and vacuum-dried at 60° C. for 48 hours to obtain carboxylated carbon nanotubes.

[0036] (3) Preparation of biosensors:

[0037] a. Pretreatment of glassy carbon el...

Embodiment 3

[0040] 1) Preparation of functionalized graphene GO-PEG: 200 mg of graphite oxide GO was added to 10 ml of DMF, and ultrasonically treated at 70 kHz for 3 hours to obtain a stable GO / DMF dispersion. 40ml SOCl 2 Add it to the dispersion liquid, reflux for 48 hours at a temperature of 80°C, and distill off excess SOCl 2 . Then 2g of PEG1000 was added to the reactant, and the reaction was refluxed at 120°C for 72 hours, washed with ethanol and water until neutral, and dried in vacuum at 60°C to obtain functionalized graphene GO-PEG.

[0041] (2) Mix 0.5g of carbon nanotubes and 100mL of strong oxidizing acid, treat it under 120kHz ultrasonic wave for 5 hours, then heat to 120°C, stir and reflux for 72 hours, dilute and wash with deionized water and filter with microporous membrane , until the filtrate is neutral, and vacuum-dried at 60° C. for 48 hours to obtain carboxylated carbon nanotubes.

[0042] (3) Preparation of biosensors:

[0043] a. Pretreatment of glassy carbon e...

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Abstract

The invention relates to a preparation method for a biosensor based on graphene/carbon nano-tube, and belongs to the technical field of material preparation. The method mainly adopts polyethylene glycol to functionally modify the graphene in order to modify the dispersibility of the graphene; and the graphene is combined with the carboxylated carbon nano-tube to form a three-dimensional net structure which is modified on a glassy carbon electrode; and acetylcholin esterase is fixed on the electrode to implement the quick electrochemical gaging for organophosphorus. The carbon nano-tube achieves the effect of molecular guide lines, and can transfer electrons to an enzyme redox center so as to largely quicken the response speed. A nanometer composite material, prepared by the method, has good biocompatibility, and brings convenience to fix biological enzyme; and the preparation method is low in cost, and is simple and fast. The biosensor, prepared by the method, has high sensitivity and good stability, can implement the quick scene quantitative detection for a large amount of samples, and can be widely applied in the environmental monitoring aspect.

Description

technical field [0001] The invention relates to a preparation method of a graphene / carbon nanotube-based biosensor, belonging to the technical field of material preparation. Background technique [0002] Organophosphorus pesticides are widely used in agriculture, industry, medicine and other fields as commonly used herbicides, fungicides, and insecticides. The number of pesticide poisoning in China is also increasing. The data show that 75.14% of the pesticide poisoning is caused by organophosphorus pesticides. produce serious pollution. Biosensors have the advantages of fast, simple, sensitive and low cost, so they are widely used in the rapid detection of pesticide residues. [0003] Graphene has good biocompatibility, unique electrical properties, obvious quantum effect, large specific surface area, high stability and strong adsorption characteristics. It is favored in scientific basic research and applied research. [0004] Carbon nanotubes (CNTS) have special electr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/403
Inventor 邱军王珊珊
Owner TONGJI UNIV
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