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Cell factor loaded nanofiber membrane and preparation method thereof

A nanofiber membrane and cytokine technology, applied in the field of cytokine-loaded nanofiber membrane and its preparation, can solve the problems of short half-life, acid and alkali resistance, easy inactivation, etc., to reduce side effects, improve curative effect, biological phase Good capacitive effect

Inactive Publication Date: 2013-05-01
SHANGHAI SIXTH PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although cytokines have the advantages of high biological activity, promoting tendon healing and reducing adhesion formation, they also have shortcomings such as short half-life, intolerant to acid and alkali, and easy inactivation in aqueous solution.

Method used

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  • Cell factor loaded nanofiber membrane and preparation method thereof
  • Cell factor loaded nanofiber membrane and preparation method thereof
  • Cell factor loaded nanofiber membrane and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] 0.1gbFGF was added into 100mL 5% dextran aqueous solution to dissolve evenly, then mixed with 1000mL 5% PEG solution using a vortex mixer, pre-frozen at -80°C for 24h and then freeze-dried. The lyophilized powder was dispersed in dichloromethane, vortexed to obtain a uniform suspension, the supernatant was removed by centrifugation, and dichloromethane (DCM) was added again, and the above operation was repeated 3 times. The bFGF-loaded dextran vitreous nanoparticles (bFGF / DGNs) were obtained after removing residual dichloromethane.

[0061] Take 100mg of the obtained bFGF / DGNs and disperse them in 2g of DCM by ultrasonic treatment in an ice bath, 0.5g of poly-L-lactic acid (PLLA) and 1g of DMF are added to the bFGF / DGNs by stirring in an ice bath, and the nanofibrous membrane loaded with bFGF is prepared by electrospinning (bFGF / DGNs-PLLA). Among them, the electrospinning process parameter conditions are: voltage 20kV, flow rate 0.04mL / min, and the distance between the...

Embodiment 2

[0069] 0.1gbFGF was added into 100mL 5% dextran aqueous solution to dissolve evenly, then mixed with 1000mL 5% PEG solution using a vortex mixer, pre-frozen at -80°C for 24h and then freeze-dried. The lyophilized powder was dispersed in dichloromethane, vortexed to obtain a uniform suspension, the supernatant was removed by centrifugation, and dichloromethane (DCM) was added again, and the above operation was repeated 3 times. The bFGF-loaded dextran vitreous nanoparticles (bFGF / DGNs) were obtained after removing residual dichloromethane.

[0070] 120 mg of bFGF / DGNs obtained was dispersed in 2 g of DCM by ultrasonic treatment in an ice bath, 0.5 g of poly-L-lactic acid (PLLA) and 1 g of DMF were added to bFGF / DGNs by stirring in an ice bath, and bFGF-loaded nanoparticles were prepared by blending electrospinning technology. Fibrous membrane (bFGF / DGNs-PLLA). Among them, the electrospinning process parameter conditions are: voltage 20kV, flow rate 0.04m L / min, and the distanc...

Embodiment 3

[0071] 0.1gbFGF was added into 100mL 5% dextran aqueous solution to dissolve evenly, then mixed with 1000mL 5% PEG solution using a vortex mixer, pre-frozen at -80°C for 24h and then freeze-dried. The lyophilized powder was dispersed in dichloromethane, vortexed to obtain a uniform suspension, the supernatant was removed by centrifugation, and dichloromethane (DCM) was added again, and the above operation was repeated 3 times. The bFGF-loaded dextran vitreous nanoparticles (bFGF / DGNs) were obtained after removing residual dichloromethane.

[0072] 110 mg of bFGF / DGNs obtained was dispersed in 2 g of DCM by ultrasonic treatment in an ice bath, 0.5 g of poly-L-lactic acid (PLLA) and 1 g of DMF were added to bFGF / DGNs by stirring in an ice bath, and bFGF-loaded nanoparticles were prepared by blending electrospinning technology. Fibrous membrane (bFGF / DGNs-PLLA). Among them, the electrospinning process parameter conditions are: voltage 20kV, flow rate 0.04mL / min, and the distance...

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Abstract

The invention provides a cell factor loaded nanofiber membrane which comprises loaded cell factors, glucan and a degradable high molecular material, wherein the cell factors are loaded in glucan vitreous body nano particles, and glucan vitreous body nano particles loaded with the cell factors are packaged in fibers of the degradable high molecular material. The cell factor loaded nanofiber membrane can effectively control slow release of the cell factors, and protect long-term activity of the cell factors during preparation and release, the biodegradable high molecular material can be degraded automatically, and the nanofiber membrane is nontoxic, has no immunogenicity, and good biocompatibility, improves a curative effect, reduces side effects, and has an important significance clinically.

Description

technical field [0001] The invention relates to a nanofiber membrane, in particular to a nanofiber membrane loaded with cytokines and a preparation method thereof. Background technique [0002] Tendon adhesion is a common clinical disease. Due to the lack of effective adhesion prevention and treatment measures, the treatment often falls into a vicious cycle of "adhesion-release-re-adhesion", which seriously affects the limb function of patients. Placing anti-adhesion membranes between the tendon and surrounding tissue has some effect in reducing tendon adhesions, but they do not have the function of promoting tendon healing and sliding. In addition, due to material or structural reasons, the wrapped tendon can only function as a physical barrier, and possible rejection and interference with tendon healing will seriously affect the recovery of tendon function. Therefore, it is of great significance to find a suitable anti-adhesion film material and structure, load anti-adhes...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L31/04A61L31/14A61L31/16A61L31/06
Inventor 范存义刘珅崔文国金拓吴飞
Owner SHANGHAI SIXTH PEOPLES HOSPITAL