Method for producing long-chain dicarboxylic acid by continuous fermentation
A technology of long-chain dibasic acid and dibasic acid, which is applied in the field of continuous fermentation to produce long-chain dibasic acid, which can solve the problems of increasing the risk of bacterial contamination and increasing costs, and achieves increased yield, guaranteed continuous production, and increased yield Effect
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Embodiment 1
[0053] Seed medium: 3.0% sucrose, 0.60% corn steep liquor, 1.0% yeast extract, KH 2 PO 4 1.2%, urea 0.30%.
[0054] Fermentation medium: glucose 3.0%, corn steep liquor 0.60%, yeast extract 0.30%, potassium nitrate 1.1%, potassium dihydrogen phosphate 0.80%, urea 0.20%, ammonium sulfate 0.15% and sodium chloride 0.10%.
[0055] There are two kinds of feed medium: one is carbasundecane; the other is a solution containing nutrient salt (glucose 0.2%, yeast extract 0.1%, corn steep liquor 0.15%, potassium dihydrogen phosphate 0.012%, urea 0.015%, The remainder is water; said percentage is mass-to-volume ratio, g / 100mL).
[0056] The shake flask seed cultivation process is to inoculate the glycerol tube strain of Candida tropicalis into a 500mL Erlenmeyer flask containing the seed medium (filling volume 50mL), the initial pH is 6.3, and culture at 29°C and 220rpm shaker for 1.5 days .
[0057] The seed tank culture process is to get the shake flask seeds and insert them in the...
Embodiment 2
[0070] Seed medium: 1% sucrose, 0.30% corn steep liquor, 0.6% yeast extract, KH 2 PO 4 0.8%, urea 0.25%.
[0071]Fermentation medium: 2.0% glucose, 0.20% corn steep liquor, 0.25% yeast extract, 0.05% potassium nitrate, 0.07% potassium dihydrogen phosphate, 0.15% urea, 0.20% ammonium sulfate and 0.12% sodium chloride.
[0072] There are two kinds of feeding medium: one is carbon dodecane; the other is a solution containing nutrient salt (yeast extract 0.1%, ammonium sulfate 0.05%, corn steep liquor 0.05%, potassium dihydrogen phosphate 0.008%, urea 0.01% , and the rest are water; said percentages are mass-to-volume ratios, g / 100mL).
[0073] The shaking flask seed cultivation process is to inoculate the glycerol tube strain of Candida tropicalis into a 500mL Erlenmeyer flask containing the seed medium (filling volume 60mL), the initial pH is 6.5, and culture at 32°C and 250rpm shaker for 1 day .
[0074] The seed tank culture process is to get the shake flask seeds and inse...
Embodiment 3
[0087] Seed medium: 3% sucrose, 1% corn steep liquor, 0.2% yeast extract, KH 2 PO 4 0.9%, urea 0.5%.
[0088] Fermentation medium: glucose 5%, corn steep liquor 0.7%, yeast extract 0.3%, potassium nitrate 0.55%, potassium dihydrogen phosphate 0.60%, urea 0.20%, ammonium sulfate 0.20% and sodium chloride 0.15%.
[0089] There are two kinds of feeding medium: one is carbon tridecane; the other is a solution containing nutrient salt (0.5% glucose, 0.15% ammonium sulfate, 0.10% corn steep liquor, 0.008% potassium dihydrogen phosphate, 0.007% urea, The remainder is water; said percentage is mass-to-volume ratio, g / 100mL).
[0090] The shake flask seed cultivation process is to inoculate the glycerol tube strain of Candida tropicalis into a 500mL Erlenmeyer flask containing the seed medium (filling volume 100mL), the initial pH is 6.0, and culture at 200rpm shaker at 30°C for 2 days .
[0091] The seed tank cultivation process is to get the shake flask seeds and insert them in t...
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